y please answer all 6) The protein content of an unknown sample was determined using bovineserum albumin (BSA) as standard. The absorbance values of the standardsolutions and of the samples are as follows.BSA concentrationAbsorbance(ug/mL)0.0050.000.0630.200.1210.400.2760.600.3360.800.4061.000.4491.20 SamplesAbsorbanceundiluted0.7151:4 dilution ratio0.4471:9 dilution ratio0.321a. Calculate the slope, y-intercept, and linearity constant for the correctabsorbance vs, concentration linear plot.b. Determine the actual or original protein concentration of the sample inug/mL.

First plot the graph of absorbance vs concentration . Then find the equation for graph using Excel.

undiluted 0.715 1:4 dilution ratio 0.447 1:9 dilution ratio 0.321 a. Calculate the slope, y-intercept, and linearity constant for the correct absorbance vs. concentration linear plot. b. Determine the actual or original protein concentration of the sample in g/mL.

undiluted 0.715 1:4 dilution ratio 0.447 1:9 dilution ratio 0.321 a. Calculate the slope, y-intercept, and linearity constant for the correct absorbance vs. concentration linear plot. b. Determine the actual or original protein concentration of the sample in g/mL.

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter16: Applications Of Neutralization Titrations

Section: Chapter Questions

Problem 16.10QAP

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Concept explainers

Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.

Question

y please answer all

6) The protein content of an unknown sample was determined using bovine
serum albumin (BSA) as standard. The absorbance values of the standard
solutions and of the samples are as follows.
BSA concentration
Absorbance
(ug/mL)
0.005
0.00
0.063
0.20
0.121
0.40
0.276
0.60
0.336
0.80
0.406
1.00
0.449
1.20

Samples
Absorbance
undiluted
0.715
1:4 dilution ratio
0.447
1:9 dilution ratio
0.321
a. Calculate the slope, y-intercept, and linearity constant for the correct
absorbance vs, concentration linear plot.
b. Determine the actual or original protein concentration of the sample in
ug/mL.

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