Wh are doing this procedures can you explain? (ex. heating or adding chemicals etc.) 1) Purification of Vitelline from Egg Yolk -Experimental Procedure Measure the volume of 3 egg yolks and mix by adding an equal volume of NaCl solution. Extract the mixture with 3-fold volumes of ether and separate the aqueous phase. Do the same procedure 3 times. Mix the sample with water and rinse. It is expected for the protein to collapse. Some more water is added in order to check whether the collapse occurs completely. The sample is centrifuged and the precipitate is dried. 2) Purification of Plasma Albumin and Fibrinogen -Experimental Procedure Add ammonium sulfate to 10 ml plasma up to 25% saturation. Separate the collapsed fibrinogen by centrifuge. Increase the saturation level to 33% by adding (NH4) 2SO4. Separate the globulin by centrifugation. Separate prodoglobulins by increasing the saturation level to 46%. Increase the saturation level up to 64% to precipitate albumin. Separate the albumin by centrifuge and store cold until use.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Wh are doing this procedures can you explain? (ex. heating or adding chemicals etc.)
1) Purification of Vitelline from Egg Yolk
-Experimental Procedure
Measure the volume of 3 egg yolks and mix by adding an equal volume of NaCl solution.
Extract the mixture with 3-fold volumes of ether and separate the aqueous phase.
Do the same procedure 3 times.
Mix the sample with water and rinse.
It is expected for the protein to collapse.
Some more water is added in order to check whether the collapse occurs completely.
The sample is centrifuged and the precipitate is dried.
2) Purification of Plasma Albumin and Fibrinogen
-Experimental Procedure
Add ammonium sulfate to 10 ml plasma up to 25% saturation.
Separate the collapsed fibrinogen by centrifuge.
Increase the saturation level to 33% by adding (NH4) 2SO4.
Separate the globulin by centrifugation.
Separate prodoglobulins by increasing the saturation level to 46%.
Increase the saturation level up to 64% to precipitate albumin.
Separate the albumin by centrifuge and store cold until use.
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