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- What are two advantages of filtration in microbial control? Explain how the filtration process works.
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- Another method of microbial control is filtration. List down at least 5 kinds of filters which could be used for this method?What are the main types of immobilization techniques used in biosensors? (with a deep explanation)briefly explain the principle behind this technique; gel-filtration chromatography
- Explain this example of the following water quality serial dilution. Note the dilution factors and how you would calculate #’s from the incubated plates. ……A serial dilution yielded TNTC in the 1:1 plate and the 1:10 plate, 10 cfu’s in the 1:100 plate and 1 cfu’s in the 1:1000 plate .Explain why the following steps are necessary during subculturing: Cooling the inoculating loop before touching the inoculum/culture ?With the aid of a diagram, explain the principles of gel filtration chromatography.
- propose a hypothesis regarding the organisms (staphylococcus, escherichia, pseudomonas, and bacillus) that will be resistant to the most disinfectants, and why? if the hypothesis is supported, what specific experimental results will be observed? what would be the independent and dependent variable? zone size, disinfectant type, colony countA microbiologist has isolated a new strain of bacteria, and is trying to characterize aspects of its growth. Three flasks of the same media have different amounts of NaCl added. Other growth conditions are identical, and the flasks inoculated at the same time. At regular intervals over a 3 hour period, culture samples are taken and turbidity is measured. Here are the absorbance values over time in a table and graphed. Absorbance (OD600) Time (min) 20% NaCl 3% NaCl 0% NaCl 0 0.05 0.05 0.05 30 0.06 0.05 0.05 60 0.075 0.06 0.05 90 0.11 0.07 0.049 120 0.25 0.1 0.048 150 0.5 0.105 0.055 180 0.7 0.11 0.055 210 0.99 0.15 0.06 240 1.22 0.18 0.055 270 1.35 0.19 0.06 300 1.5 0.2 0.06 330 1.6 0.25 0.062 360 1.7 0.3 0.06 Based on these data, which of the following can be determined about this bacterium? It is psychrophilic. It…Which of the following statements regarding low temperature storage is FALSE? Question options: freezing inhibits microbes through the unavailability of water freezing causes microbial death largely through DNA denaturation all of these statements are TRUE refrigeration functions largely by reducing the metabolic rate of microbes psychrophiles are generally not effectively inhibited by refrigeration many bacteria can survive freezing for years at a time
- Heat-labile material to be introduced to culture medium was filtered using membrane filters with unknown pore size. Effect: Rationale:https://courses.lumenlearning.com/microbiology/chapter/controlling-microbial-growth/ Read this Article about Controlling Microbial Growth and answer the following questions: Which is most effective at removing microbes from a product: sanitization, degerming, or sterilization? Explain. What are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name at least two factors that can compromise the effectiveness of a disinfecting agent.A continuious, stirred-tank bioreactor with an actively growing culture of bacteria is operating at steady state. While holding all other operating parameters constant, the flowrate is suddenly decreased. A new steady state is achieved. When comparing the new steady state conditions to the original steady state conditions, what can be said ? (select the best answer from below). any of the answers given here could be true, we'd have to know the initial steady state Substrate concentration went up and productivity could have gone up or down Substrate concentration went down and productivity could have gone up or down Substrate concentration went up and productivity went down Substrate concentration went up and productivity went up Substrate concentration went down and productivity went up Substrate concentration went down and productivity went down