What is the best way to reduce “bleedthrough” when fluorescein and rhodamine are used simultaneously? A.Using a stronger excitation for rhodamine. B.Using an optimal combination of fluorescence filters. C.Using less rhodamine than fluorescein. D.By imaging each fluorescent molecule separately and waiting several seconds between

Principles Of Radiographic Imaging: An Art And A Science
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ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Chapter11: Filtration
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What is the best way to reduce “bleedthrough” when fluorescein and rhodamine are used simultaneously?

  • A.Using a stronger excitation for rhodamine.
  • B.Using an optimal combination of fluorescence filters.
  • C.Using less rhodamine than fluorescein.
  • D.By imaging each fluorescent molecule separately and waiting several seconds between each image, to ensure that all fluorescence has been emitted.
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