What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?
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What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?
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- DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?Apple puree was analyzed for petulin by HPLC-MS-MS after SPE clean-up. The procedure was 10.0g of puree + 10μl of a 10μg/ml solution of isotopically labeled petulin as internal standard were treated with 10.0ml of pectinase and acetic acid, centrifuged, and filtered. Four ml of the filtrate was passed through a SPE cartridge. The petulin was eluted with 2.0ml of ethyl acetate. The sample was evaporated to dryness and the residue dissolved in 1.0ml of the mobile phase. The analyte signal was 127 and the internal standard 197. Calculate the concentration of petulin in the sample in μg/g (RRF=1).Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…
- What does the phrase "pipetting up and down" mean and why is this technique used? The phrase "pipetting up & down" means to triturate. Th technique is used to mix (or homogenize) a solutic 7 On what part of a microcentrifuge tube should you write a label? Describe the order in which you filled the tubes in Step 2 of Procedure 4. Did this order result in maximum efficiency? If not, what order would be most efficient? 9 What does the phrase "spinning down" mean and why is this technique used? The word 'spinning down" means, To diminish in energy i to slow down o out; to be gradually ended or concelled. It is used to reduce its spin speed from that required for reading and writing. 10 Why was the comb placed in the middle rather than at one end of the gel for this electrophoresis experime- The comb is in the center of the gel Since the dyes used have positive or negative charges and can therefore migrate in differenConfluence cells added onto 100-wells plate, 2 ml trypsin added onto cells and 6 ml media added to dilute trypsin, 1 ml was used for counting, and average number of Cells counted was 24(DF 2) find out the the number of cells/ml and total number of cells in 7 ml? How many cells you need for 100 wells, assuming Number of cells/well is 10 to the power 4 cells?a. What is the total dilution of Tube #4? Express the answer in exponential format. b. You plated 1 mL of the Tube #4, After incubating, you counted 500 colonies on the plate. What is the concentration of Tube #0? include units. c. How could you change the experiment in part B to get a plate in the countable range? Be specific about any dilution factors and/or plated volumes you would change.
- Observe the following Plate counts and then determine the correct number of CFU/ml Plate 1 = 564 colonies at 10^-5 dilution Plate 2 = 422 colonies at 10^-6 dilution Plate 3 = 317 colonies at 10^-7 dilution Plate 4 = 93 colonies at 10^-8 dilution 93 x 10^10 CFU/ml 9.3 x 10^-9 CFU/ml 93 x 10^9 CFU/ml 93x 10^8 CFÜ/ml 93x 10^-8 CFU/ml asap pleasePlease answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurredSample Station 5 This is an MRVP broth. The reagents A and B for the VP test have been added. A. What observations can you make about this test? B. What interpretation can you make about this organism's ability to ferment glucose?
- Serial dilution = previous dilution x dilution 11 mL [2] 3 mL Colony count: 100 0.1 mL sample [1] 9.9 mL 5 mL 0.1 ml. Cokony count Dilution: 10-1 10-3 [3] 150 Dilution factor (DF): [4] [5] [6] Compute for the CFU/mL of the sample. Show your solution and express your final answer to 2 significant figures.Answer the questions. Cite your reference. You received three swabs from a wound discharge, and you placed them on enrichment media. You noticed growth was distributed throughout all the media; there was abundant growth toward the surface of the medium in one tube, whereas other tubes showed an equal distribution of growth throughout the tubes. Explain why this happened.Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.