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What is the limit of resolution for a bright-field microscope?
What defines this limit?
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- On a particular microscope, the numerical apertures of the condenser and the high dry power objective lenses are 1.25 and 0.65 respectively. You are supplied with a filter that selects a wavelength of 500 nm. A) What is the limit of resolution on this microscope when using the high dry power lens? (show your calculations) B) Will you be able to distinguish two points that are 260 nm apart as being separate or will they blur into one? (explain your answer)Define the minimal separation distance of a microscope and give its equation.What is the magnification power of transmission electron microscope?