What is the name of the method you used to isolate protein from bacterial sample
Q: How do you make liquid bacteria culture?
A: It is important for growing more numbers of bacteria to isolate enough plasmid DNA for experimental…
Q: How do you prepare 50 ml of 20 mg/ml proteinase K from 50 mg/ml stock solution
A: Proteinase K is a serine proteinase enzyme used to digest proteins. It is produced from a…
Q: Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?
A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…
Q: What is the purpose of the bacterial capsule? Answer in not more than 3 sentences.
A: Prokaryotic calls contain addition protective layer outside of the bacterial cell wall. It is…
Q: tate the apparatus used view small samples
A: The world is full of organisms. Organisms have developed from minute single celled structures to…
Q: Write introduction about Identification of bacteria &Bacterial Staining?
A: Bacteria are unicellular prokaryotic microorganisms (nucleus is not enclosed in a nuclear membrane)…
Q: Why is oil immersion necessary to see bacteria in the laboratory?
A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…
Q: What tools are used to transfer bacteria
A: Ans - An inoculation loop is used to transfer bacteria for microbiological culture. Petri dish.…
Q: What is another test you could use to determine the amount of protein?
A: The nutrients can be classified into three groups, water, micronutrients, and macronutrients. Water…
Q: Examples of bacteria observe in the microscope using Gram stain, Ziehl-Neelsen, and Kinyoun Method.
A: Ziehl–Neelsen staining is a type of acid-fast stain, first introduced by Paul Ehrlich. Ziehl–Neelsen…
Q: List 6 common bacterial shapes.
A: Bacteria is a type of microorganism and consists of a single, prokaryotic cell. They lack a…
Q: You are using bacterium E.coli to synthesize a protein from recombinant DNA. The protein remains in…
A: Escherichia coli is one among the organisms of selection for producing recombinant proteins. It's…
Q: What color is a GRAM NEGATIVE bacteria? purple pink
A: Microscopic, single celled organisms which are found in every environment in millions are known as…
Q: Discuss why you should incubate bacterial plate upside down with the lid at the bottom:
A: Management of the culture plates is important in the microbiology laboratory. It is studied under…
Q: Which one of the following is NOT considered a biohazard? old bacterial cultures slides…
A: Biohazard is defined as any biological substance that can pose or cause a threat and harm to biotic…
Q: Gram Stain Results: Staphylococcus aureus (a Gram-positive coccus) Escherichia coli (a Gram-negative…
A: Staining is the method of producing different colours that obtained by the reaction of reagents with…
Q: microbiology
A: For to obtain cultures and Sensitivity faster, depends on targeted bacterial strain. The media…
Q: When viewed under a microscope, bacteria appear as red rods after they have been stained. Explain a…
A: Introduction Bacteria Are Minute, Single-celled Organisms That Can Be Found In Large Numbers In All…
Q: what is the gram staining procedure for e-coli
A: Gram staining is the laboratory technique which is used to differentiate between the morphology of…
Q: Create a flowchart using the given reagents and equipment to perform bacterial DNA extraction. ●…
A: Introduction : DNA extraction is a very skilled process and it requires these four steps in…
Q: I missed a couple of my labs due to covid and am struggling to teach myself how to do serial…
A: The last two years have been extremely difficult because you can still learn and understand the…
Q: Explain how to perform a serial dilution
A: Serial dilution is stepwise dilution of a solution keeping the dilution factor constant in each…
Q: You and a partner conduct the acid-fast staining using the same two bacteria. You know that there…
A: Acid-fast staining is a differential staining technique that proves to be an important diagnostic…
Q: What is the order of staining in microbiology
A: Crystal Violet - Primary stain ( Stain the cell wall of the microorganisms) Gram's iodine - Acts…
Q: Describe three problems associated with using the standard plate count method for determining the…
A: Typically, an accurate count of bacterial colonies is required for biological processes. Microbial…
Q: Based on the photo, describe the bacteria observed from the different staining technique. -…
A: Microbiology was a blessing in the eradication of mass-destructive epidemics such as rabies,…
Q: lain how a wire loop and wire needle is used in microbiology laboratory.
A: A laboratory dedicated to the culturing, examination, and identification of microorganisms including…
Q: Why was knowledge of how to obtain a pure culture important for development of the science of…
A: A pure culture is one that contains only one species of an organism. The procedure of pure culture…
Q: What technology is available for faster bacterial culture?
A: BASIC INFORMATION BACTERIA It is one of the type of biological cell. They belong to prokaryotic…
Q: Does fructose can produce a positive result in Seliwanoff Test? Elaborate your answer
A: Seliwanoff’s test is a test for carbohydrates that can distinguish between aldose and ketose sugars.…
Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…
A: Serial Dilution -- Serial dilution are used to calculate the concentration of microorganism .It…
Q: trospray mass spectro
A: The amino acid of any of a group of organic molecules that consist of a basic amino group (―NH2), an…
Q: If you had a liquid culture containing three different species of bacteria, how could the use of a…
A: A microbiological culture, also known as a microbial culture, is a technique for multiplying…
Q: How do we reconcile the number of colonies in the plates to the actual number of bacteria in the…
A: Microbes may enter the body in a variety of ways and cause infection everywhere, but antigen and…
Q: What is gram staining Step by step procedure Explain its basis and how it differentiate bacteria use…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: If you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40…
A: In the question it is asked that they want to plate out 40 bacteria, and this count comes under the…
Q: How do you make bacteria in the laboratory?
A: Bacteria are microscopic single-celled organisms that thrive in diverse environmental conditions.…
Q: What are the advantages and limitations of studying bacteria by means of the Culture method?
A: Introduction Culture:- It is a method of growing microorganisms, tissue cells, or other living…
Q: What is the principle of Grams staining based on the cell wall composition of bacterial cell.
A: Bacteria are a kind of organic cell. They comprise an enormous area of prokaryotic microorganisms.…
Q: You perform the Methyl-Red (right) and Voges-Proskauer test (left). Interpret the results. Which…
A: Voges-Proskauer (VP) is a test used to determine the acetoin in broth culture of bacteria. VP broth…
Q: Explain why an isolated colony and a pure culture are not the samething.
A: The isolation techniques: It consists of separation of bacterial cells from other cells from mixed…
Q: What are the general rules in grams staining classification of bacteria?
A: In microbiology, Gram staining is the most widely utilised, vital, and widely used differential…
Q: staining method would you use to verify that the isolates are not Mycobacterium species? Describe…
A: Staining is a method of coloring biological tissues to increase the contrast. This technique is…
Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…
A:
Q: How to make a bacterial cell competent
A: Bacterial cells can be made competent either naturally or artificially. Competent cells are cells…
Q: how else could bacteria in soil be characterized and identified other than gram staining
A: Gram staining method is one of the important ways to identify bacteria. This principle is based on…
Q: Explain, in your own words, why it is important to perform serial dilution when trying to quantify…
A: Serial dilution is type of dilution used in field of microbiology to identify the cell count.
Q: Exaplain in your own words how to Accumulate data and characteristics of the unknown until a…
A: Microorganisms like bacteria are smaller in size such that they are not visible through the naked…
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- What is the purpose of staining bacteria? What is the purpose of collecting bacteria from different sources and staining them. What can you learn from this type of experiment?How do you make liquid bacteria culture?The process of collecting characteristics of your unknown bacterium can be helped by the use of a dichotomous key. A dichotomous key is a flowchart that can be used to identify an organism. a) in your own words explain how to create and use a dichotomous key to identify unknown bacteria
- Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE DILUTIONS in series? Consider the accuracy of the pipetting so that you don’t make a single dilution that is too large.How do we reconcile the number of colonies in the plates to the actual number of bacteria in the original sample? Count Plate MethodYou've been assigned with isolating a certain species of bacteria from a mixed culture in order to characterize and identify the strain. Which technique(s) is/are the BEST to utilize? How will the procedure be carried out? Explain.
- The images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?You are tasked to isolate a certain species of bacteria from a mixed culture to further characterize and identify the strain. Which technique/s is Best to perform? How shall the procedure be carried out? Explain.What are the advantages and limitations of studying bacteria by means of the Culture method?
- a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteriaWhy is bacterial culture useful to grow bacteria?You perform the Methyl-Red (right) and Voges-Proskauer test (left). Interpret the results. Which methods would you use to determine is the bacterial sample is E. coli or P. vulargis? What results would help you to decide?