What kind of buffer would you make with a optimal activity at ph 4.30? Materials Available In the LabDI WaterAcetate pKa = 4.76o 1.5 M acetic açid solutionCH,COOHo Solid sodium acetate (molar mass 82 g/mol)NACH,CO0Tris (pKa = 8.10)o Solid tris chloride (molar mass 157.6 g/mol)(HOCH,),NH,Clo Solid tris base (molar mass 121.1 g/mol)(HOCH,),NH,Phosphate (pK1 = 2.14, pK2 = 6.86, pK3 = 12.4)1.0 M phosphoric acid solutionH3PO,1.5 M monosodium phosphate solutionNaH,PO,1.25 M disodium phosphate solutionNa,HPO,2.25 M trisodium phosphate solutionNa, PO,Questions1. You are interested in studying a protein with optimal activity at pH 4.30.a. What kind of buffer would you make? Justify your answer.b.What is the weak acid? What is the conjugate base?

A buffer is defined as an aqueous solution that has a mixture of a weak acid and its conjugate base…

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Biochemistry

What kind of buffer would you make with a optimal activity at ph 4.30?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

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What kind of buffer would you make with a optimal activity at ph 4.30?

Materials Available In the Lab
DI Water
Acetate pKa = 4.76
o 1.5 M acetic açid solution
CH,COOH
o Solid sodium acetate (molar mass 82 g/mol)
NACH,CO0
Tris (pKa = 8.10)
o Solid tris chloride (molar mass 157.6 g/mol)
(HOCH,),NH,Cl
o Solid tris base (molar mass 121.1 g/mol)
(HOCH,),NH,
Phosphate (pK1 = 2.14, pK2 = 6.86, pK3 = 12.4)
1.0 M phosphoric acid solution
H3PO,
1.5 M monosodium phosphate solution
NaH,PO,
1.25 M disodium phosphate solution
Na,HPO,
2.25 M trisodium phosphate solution
Na, PO,
Questions
1. You are interested in studying a protein with optimal activity at pH 4.30.
a. What kind of buffer would you make? Justify your answer.
b.
What is the weak acid? What is the conjugate base?

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