What level of RF in serum is clinically significant? Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the qualitative test? Describe how the RF concentration is computed?
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What level of RF in serum is clinically significant?
Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the qualitative test?
Describe how the RF concentration is computed?
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- What is the principlels of in-dose test?Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the qualitative test?What is the purpose of the RPR (RAPID PLASMA REAGIN ) Test? Why is a qualitative test performed before a semi-quantitative test? Why is it necessary to rotate the slide/card?
- 6) 1 mL of supernatant is required for a procedure. The final colored solution proves to be too high to read accurately on the spectrophotometer. 100 μL of supernatant and 900 μL of distilled water are substituted for the original supernatant and the procedure, run as before. The reading from the standard curve is 46 mg/dL. What is the actual amount of substance in the patient serum?In a tabulated form enumerate the anticoagulants for blood chemistry analysis, its action, the correct proportion to the amount of blood, and the different laboratory tests that could be done for each anticoagulant. What is the significance of the order of draw? Is it still applicable to date?Discuss the medical application of the Benedict’s test? What other test(s) are used in parallel to Benedict’s test?
- Please explain the theoretical background (positive color reactions and what is being detected) involved in the different qualitative test for proteins that has been isolated intact or hydrolyzed. Please directly answer the theoretical background (positive color reactions and what is being detected) of each test GENERAL: BIURET - NINHYDRIN SPECIFIC XANTHOPROTEIC TEST - MILLON'S TEST - HOPKINS-COLE TEST - SAKAGUCHI TEST - LEAD ACETATE TEST - NITROPRUSSIDE TEST - FOLLIN'S REACTION - SULLIVAN'S TEST - PAULY REACTION - LIEBERMAN'S TEST -The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of culture to the first tube and mix. You get distracted, and transfer 0.1 ml to the third tube instead of the second. You perform the rest of the series as described.What is the clinical significance of ESR determination? Differentiate Wintrobe from Westergreen regarding accuracy of the test in a tabulated order. NOTE: Kindly asnwer all the questions. Thank you!