What would you expect to occur if you streaked 1/2 of an agar plate with S. epidermidis and the other half with Penicillium and then incubated for one week? Relate this prediction to your results in Exercise 1.
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Microbial Physiology
Microbial physiology is the branch of microbiology that is associated with studying the physiology of fungi, bacteria, and viruses. It is an important field of science concerning functional genomics and metabolic engineering.
Chemotaxis
The organism’s movement in a particular direction as a response to certain chemicals that are available in the environment is defined as chemotaxis. In brief, it can be defined as the occurrence of an organism’s movement in response to a certain chemical stimulus.
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- After incubating for 24-48 hours, you retrieve your 4 agar plates for the osmotic pressure exercise from the 37°C incubator. You observe the following results: • Sa = Staphylococcus aureus • Pv = Proteus vulgaris Sa For each organism, you should indicate in the text box below: 1. At what salt concentrations (osmotic pressures) you saw growth. 2. The classification term you would use in order to classify the organism based on osmotic pressure. Choose your term based upon where you observed growth; not optimal growth. Each organism has only one classification term associated in describing its growth pattern(s). You will need to use the osmotic pressure classification descriptions and terms outlined in your lab manual.Using the data provided below and the information from the Activity 3's Experiment section, answer the following questions: Questions 12-15. Light tan represents the spots where the inoculum was plated, but showed no growth. Dark Tan represents the spots where inoculum was plated, but did show growth. UMM1. If you expose E.coli to UV light at 30 seconds, 1 minute, 3 minutes and 5 minutes, what are your expected results? 2. If you expose. B. cereus to UV light at 30 seconds, 1 minute, 3 minutes and 5 minutes, what are your expected results? 3. If you do not remove the cover of the plate before exposure to UV light at 5 minutes for E.coli and B. cereus, what are your expected results? Help with 1,2 & 3 please
- Observing the actual growth on your incubated plates, complete the table below. Describe the growth as a heavy lawn, isolated colonies, or absent. Indicate the presence or absence of GFP by entering yes or no.D Question 37 Based on the data below, how much more UV resistant was B. licheniformis relative to S. aureus? (All controls had growth.) - covered with lid Organisms Exposure 20 sec Staphylococcus aureus 5 sec + 20 sec Bacillus licheniformis + 10 sec 80 sec times to UV light 40 sec 2.5 min 160sec 5 min 10 min 5 min 20 min 10 min 40 min 10 min + 40 minDescribe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. (Don't have to write the incubation period) ~ Only describe how would it look like on the Agar: Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient Agar
- explain the Kirby Bauer method of antimicrobic sensitivity testing in your own wordsHow can I explain the results of my findings in lamen terms? 1 - Name of Test Type of Medium 2 - Color Before Incubation 3-Color After Incubation? 4 – Results Positive/ Negative? 5 - Reagents Added Lactose Fermentation test Phenol Red Lactose (PRL) Red Color: Yellow Gas Bubble: Yes Positive Phenol Red to detect color change (added) Glucose Fermentation Test Phenol Red Glucose (PRG) Red Color: Yellow Gas Bubble: Yes Positive Phenol Red to detect color change (added) Citrate Utilization Simmons Citrate Green Color: Blue Positive Bromthymol Blue (added) Indole Test Media = SIM Clear Indole: Color: Clear Negative For Indole, we add Kovac’s Hydrogen Sulfide Test Media = SIM clear Hydrogen Sulfide: Color: Clear Negative Iron already added Methyl Red Test (MR test) Media = MRVP Broth Clear Color: Yellow Negative We add Methyl Red Reagent Voges-Proskauer Test (VP test) Media = MRVP Broth Clear…A culture is incubated for 10 hours. 1 hours after inoculation it reached the exponential growth phase. At this time point the cell density was 1x10^4 cells/ml. 5 hours after inoculation (still during the exponential growth phase) the cell density was 1x10^7 cells. Calculate K and g(t). The growth constant (k) is minute. (round to 3 decimal points) The generation time (gt) is minutes. (round to whole number)
- Can a P1000 micropipette be used to accurately pipet a glycerol solution? Why or why not? What about a serological pipette?In this exercise, the action of bactericidins will be evaluated by __________. In this exercise, the action of bactericidins will be evaluated by __________. observing color change counting the number of colonies on agar viewing lysozyme activity measuring turbidity measuring microbial metabolic activityAnswer each of the questions below using the plate images provided. Nutrient Agar 1 EMB Blood Agar 1. How many colonies are on NA plate 3?. 2. How many bacteria were transferred to NA plate 3?. 3. How many colonies are on NA plate 2? 4. How many bacteria were transferred to NA plate 2?. 5. How would you describe the growth on NA plate 1?. 6. How many colonies grew together on NA plate 1? 7. How many bacteria were transferred to NA plate 1? Nutrient Agar 2 8. How many bacteria collected from the source? (Hint: What fraction of tube 1 was spread on NA plate 1?) 9. Were any of the bacteria from the source Gram-negative? How can you tell?. 10. Could any of the bacteria from the source ferment lactose? How can you tell? Nutrient Agar 3 11. Were any of the bacteria from the source hemolytic? How can you tell?. 00