Which of the following is most needed to determine the DNA quantity using the eyeball method? A DNA ladder B positive control C negative control D loading buffer
Q: You are using a spectrophotometer to determine the concentration of DNA in a PCR product, but your…
A: The concentration of DNA and RNA should be determined by measuring the absorbance at 260 nm (A260)…
Q: Which of the following enzymes are needed to introduce foreignDNA into a vector?a. DNA gyrase and…
A: The tightly packed genetic material located in the nucleus of a living organism which is made up of…
Q: The enzyme that removes the RNA primer from the Okazaki fragment is: A DNA ligase B DNA gyrase (c)…
A: The double standard DNA molecule is produced by the action of DNA volume range and the process…
Q: Which of the following ingredients does not belong in a sequencing reaction? (A ddNTPs B Primer C…
A:
Q: Which of the following technique is used for the amplification of DNA fragments?a) AFLPb) RFLPc)…
A: RFLP (restriction fragment length polymorphism) is a technique used to exploit the variation in the…
Q: Which of the following enzymes are needed to introduce foreign DNA into a vector? a. DNA gyrase and…
A: DNA (deoxyribonucleic acid) is the genetic material in most organisms except few viruses.…
Q: Determine the chemical reagents utilized in banana DNA extraction and their roles. What role does…
A: 3. Liquid dishwashing soap This helps split apart the cell membrane made of lipids and the cell…
Q: In the "Indexing DNA" method of n k-mer table, the maximum number of compares when the k-mers are…
A: Indexing of genome is done in order to index all the small sequenes within the large genome.The…
Q: Which set of DNA fragments below would require a polyacrylamide gel to effectively separate the…
A: Polyacrylamide gel electrophoresis is a technique used to separate proteins or nucleic acids on the…
Q: What allows the peaks to be different colors?
A: DNA sequencing refers to the determination of the nucleotide sequence in the DNA molecule. Automated…
Q: During gel electrophoresis, DNA fragments are separated such that the longest DNA samplas migrate…
A: It is false The true statement is- during gel electrophoresis DNA fragment are separated such that…
Q: If a sample of double-stranded DNA gave the following results, what is the concentration (ng/uL) of…
A: The absorption value of 1 at A260 corresponds to 50 ug/ml So, the value of 1.637 at A260 corresponds…
Q: Which of the following is NOT needed to perform a PCR reaction? A) DNA primers B) deoxyguanosine…
A: Components required for PCR include a DNA sample which acts as a template , DNA primers, free…
Q: Which of the following is most needed to determine the DNA quantity using the eyeball method? -DNA…
A: DNA ladder is the most needed thing to determine the DNA quantity using the eyeball method. As we…
Q: When you isolate DNA, what is the purpose of the hot water and the detergent in the activity?
A: The basic principle related to DNA isolation is to distrup the cell wall, cell membrane and nuclear…
Q: What should you do when loading the DNA sample into the gel well? A. Ensuring that the DNA samples…
A: Gel electrophoresis is a technique used in the biotechnology lab to separate the DNA fragments…
Q: ripe banana a better sample compared to an unripe banana for DNA extraction
A: Raw bananas are often preferred for DNA extraction because they have high number of cells per…
Q: Aside from the sample and assuming proper AGE protocol, which of the following is most needed to…
A: Agarose gel electrophoresis is an important technique used in molecular biology research. In case…
Q: Identify the CORRECT sequence of processes that takes place during DNA extraction. - ethanol…
A: Introduction : DNA: DNA Extraction Is A Method For Isolating DNA From A Biological Sample.…
Q: Which of the following will lead to DNA cutting more frequently? Choose all that apply.
A: The restriction enzymes are enzymes that cut the DNA at specific nucleotide sequences called the…
Q: What is the use of Liquid soap in DNA extraction?* A. binds cell membrane and nuclei B. dissolves…
A: DNA extraction is a process of extracting DNA from the cell for further experiments. It is a method…
Q: Which of the following best describes the process of DNA seqencing.
A: DNA is mainly made up of the nucleotide and also contains genetic information in the genes. Every…
Q: When running a gel during a Sanger sequencing, the following results were obtained: I I Find the DNA…
A: Sanger sequencing is a technique which is used to determine the order of the four nucleotide bases…
Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…
A: Biotechnology is the use of our understanding of biological processes to develop useful applications…
Q: Can you please interpret and discuss and mark, this gel electrophoresis results. DNA ladder sizes:…
A: A molecular-weight/ size marker, also referred to as a DNA ladder, is a set of standards that are…
Q: The production in a pcr reaction is: nucleotids double strand circular dna double stranf linear…
A: PCR stands for polymerase chain reaction.
Q: The ingredients used in PCR will contain all of the following except: Group of answer choices A.…
A: The PCR or Polymerase chain reaction is a widely preferred laboratory technique which is used to…
Q: how enzymes are involved with proofreading newly formed DNA molecules. 1. DNA polymerase does not…
A: In proofreading process, The polymerase checks whether the recently added base has matched…
Q: In typing DNA from a sample found at a crime scene, how can a DNA mismatch prove that a suspect is…
A: DNA typing is also known as DNA fingerprinting. It is a method used to distinguish between…
Q: The enzyme that removes the RNA primer from the Okazaki fragment is: DNA pol III DNA ligase…
A: Introduction: DNA is the hereditary material that defines every cell. It is found within the nucleus…
Q: hich of the following ARE part of a typical PCR reaction mixture? DNA ligase dNTPs (mix…
A: Ans- dNTPs(mix ofdeoxynucleoside tri-phosphates )
Q: Give an example of application of recombinant DNA in the following fields: crop production,…
A: Answer - Recombinant DNA (r DNA) is a technique by which we cut and paste DNA sequence of interest…
Q: After a piece of DNA is sequenced, what type of analyses can be performed?
A: After the sequence of the DNA have been found many analysis are performed on the DNA.
Q: hematic diagram for the procedure in doing DNA extraction, isolation and quantification
A: The deoxyribonucleic acid isolation method frees deoxyribonucleic acid from the cell and {so}…
Q: of the following DNA models is accurately labeled? a. b. A d. 3'
A: The molecule found inside cells that holds the genetic information necessary for an organism's…
Q: An individual’s unique set of_______ can be used in DNA profiling. a. DNA sequences c. SNPs b. short…
A: Introduction In the genome, there is usually two types of nucleic acid sequence present. One is…
Q: State the function of each chemical/components below in DNA extraction Salt: Detergent:…
A: Breaking cells open to release the DNA. Separating DNA from proteins and other cellular debris.…
Q: You have two DNA samples, A and B. A 1/10 dilution of sample A had an absorbance at 260nm of 0.12. A…
A: Nucleic acids strongly absorb UV light with wavelengths of 260 nm due to the resonance structure of…
Q: Which of the following statements about DNA probes are false? a. Probes can be labeled with a…
A: In the biotechnology special in recombinant DNA technology the probe is very much important and it…
Q: For DNA amplification using PCR to occur, which of the following are needed? A. DNA primers…
A: The term polymerization stands for the production of a large chain or network-like molecules, from a…
Q: For a DNA sample, the OD value at 260nm = 0.125, and the OD value at 280nm 0.065. %3D Purity of DNA…
A: Purity of a DNA sample is defined as the ratio of absorbance at 260 nm and 280 nm. If the ratio…
Q: Which of the following is not essential to carry out the polymerasechain reaction?a. primers b. DNA…
A: PCR is the polymerase chain reaction and is used to make various copies of a specific region of DNA…
Q: Which of the following is the correct order in extracting the DNA? I. Washing of DNA II. Tissue…
A: DNA act as genetic material in most of organism . It is two stranded , ladder like structure . DNA…
Q: DNA bands from each sample lane of the gel electropherogram below.
A: Key points: Gel electrophoresis is a technique used to separate DNA fragments according to their…
Q: Which of the following tools of DNA technology is incorrectlypaired with its use?(A)…
A: As in the recent times, the approach towards genetics has gone up a high level. Various techniques…
Q: In the "Indexing DNA" method of n k-mer table, the maximum number of compares when the k-mers are…
A: The process of indexing a genome is similar to that of indexing a book.
Q: The last steps of DNA extraction include washing it using ethyl alcohol and drying the DNA pellet.…
A: DNA is the genetic material present in the cells of living organisms.
Q: A. Changes and damages to the DNA is permanent and has no remedy. B. Damage to the DNA can be…
A: DNA or Deoxyribonucleic acid, is the hereditary material that composed of two polynucleotide chain…
Q: The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is dependent…
A: Agarose gel electrophoresis: It is used for the separation of DNA molecules that are larger than…
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- Which of the following is most needed to determine the DNA quantity using the eyeball method? -DNA ladder -loading buffer -positive control -negative controlWhy is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…When gel electrophoresis is undertaken, one well always contains DNA-marker molecules. The purpose of these markers is to Question 30 options: help visualize the DNA indicate when the DC current is turned on serve as a guide to the length of the fragments in the other wells indicate when the DNA has completely crossed the gel serve as a channel for ethidium bromide
- When gel electrophoresis is done correctly, which of these DNA molecules would move the least through the gel? Group of answer choices: 1000 bp molecules 6 Kbp molecules 3 Kbp molecules 2000 bp moleculesExamine the pGLO plasmid DNA solution with the UV lamp. Note your observations. Using a micropipettor, withdraw 10 ul of plasmid and mix it into the cell suspension of the +PGLO tube by pipetting up and down three times. Close the tube and return it to the rack on ice. Also close the -PGLO tube. Do not add plasmid DNA to the -pGLO tube. Why not?During a typical gel electrophoresis set up (negative pole at the top), which DNA fragment would be nearest the top after separating the DNA fragments? 10 kb 100 kb 2,500 bp 2 bp
- Which is the odd one out ? For the rest, explain the concept/process/technique they are involved with. DNA polymerase primers DNA RNA ddNTPsYou are provided with coiled DNA and plasmid DNA that you subject to gel electrophoresis. Draw this gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. Exact fragment sizes are not important.K What is an example of positive feedback? sweating when you are hot increased respiration during exercise decreased blood glucose levels when insulin is released maintenance of blood pH levels labour contractions during birth
- if you have the following sequence of DNA 5' ATTGCGGAGCCTCGAT 3' do the following:What is the relationship between fluorescence intensity of a spot and the amount of DNA in a sample for a Virochip DNA microassay? From the following which is the best choice Less DNA results in greater fluorescene since the laser passes through he sample eaiser More DNA equals to more intense fluorescence since the DNA makes a layer to refelct the laser light More DNA equals to more intense fluorescence since of more dye on the DNA is fluorescene More DNA results in to more fluorescence since there's more DNA to be excted by the laser None of the aboveYou digest 4 uL of plasmid DNA that is 50 ng/uL concetration in a total volume of 20 uL. You run 10 uL of the digest on teh gel. You then do a DNA purification protocol with a Zippy prep on the remaining digested DNA. You elute the DNA in a 25 uL. A 2 uL ssample has the concentration of 2 ng/uL. What is the DNA yield? YIELD = How much dna came out of the column/how much DNA was loaded onto the columnRemember to subtract amount of DNA run on gel from total digested DNA to get hw much DNA was loaded onto the columnAmount that came out of the column equals the volume of the eluted DNA times the concentration of the purified DNA