Which of the following statements is correct about column chromatography? a) Resolution improves as column length increases b) Mobile phase is a porous solid material kept in the column with appropriate chemical characteristics c) Stationary phase is a buffered solution that percolates through the mobile phase d) Large proteins exit the column faster than tiny proteins
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- In which type of chromatography lipids are carried up a silica gel coated pate by a rising solvent front, less polar travels farther than the more polar ones?a) Absorption chromatographyb) Thin layer chromatographyc) Gas-liquid chromatographyd) HPLCIn which type of chromatography, solvents of increasing polarity are passed through a column of silica gel?a) High performance liquid chromatographyb) Thin layer chromatographyc) Adsorption chromatographyd) Gas-liquid chromatographyA negative result with MPO stain would be seen with what cell type? Question 5 options: A) neutrophils B) lymphocytes C) eosinophils D) basophils
- When loading samples onto a gel, why should expelling all the liquid in the tip be avoided? a)All the sample could be forced out from the well in which it was loaded. b)The extra liquid would end up in the buffer and this would distort the migration of the sample. c)The gel could tear and distort the migration of the sample. d)An air bubble could result which would distort the migration of the sample .Ethanol has been carried over into your DNA sample (two answers possible here, select both of them) a) The column tip touched the column flow through b) The cells stayed in the lysis buffer for too long c) You didn't add enough wash buffer d) You did not centrifuge a final time after the final washWhich of the alternatives below indicates techniques used both to purify and to concentrate proteins, without losing the three-dimensional structure? a) diafiltration, affinity chromatography, centrifugation b) diafiltration, salting out, affinity chromatography c) precipitation with organic solvent, diafiltration, centrifugation d) centrifugation, precipitation with organic solvent, salting out
- 11. ● Possible methods for the quantitative determination of sodium bicarbonate for injection are: A) Acidimetry B) Alkalimetry C) Complexometry D) Iodometry E) Refractometry ● Calcium chloride is available in dosage forms: A) Powder for preparation of injections B) Solution for injection 10% in ampoules C) Tablets of 0.3 g D) Solution for injection 25% in ampoules E) Dry dosed powderWould a syringe filter with a pore size of 0.22 micrometers be able to completely remove all microorganisms including viruses? Explain why or why not?The sediment observed in stained blood smear caused by:a) Overstrainingb) More Haematoxylin in the mixturec) Smear was not fixedd) Using non- filtered staine) Using old stain
- The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m, end text. In an experiment, students submerged the cube in red dye for 121212 hours. The red dye permeated 1\, \text{mm}1mm1, start text, m, m, end text on each side, as indicated by the shading in the figure. Volume of a rectangular solid: V = lwhV=lwhV, equals, l, w, h Calculate the volume of the agar cube that remained unpenetrated by the red dye.In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4 a) Why was the pH of the coating buffer so different?b) What is “coating buffer” for an ELISA? Does the buffer molecule usedmake sense based on the desired pH?In an ELISA procedure the samples are incubated and the ELISA plate iscovered with parafilm and placed in a humidified chamber to preventevaporation of the small liquid volumes in the wells.c) How would your results change if you did not incubate in a humidifiedchamber?