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- An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography.The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.Why is it important to select immiscible solvents when performing liquid-liquid extraction? In general, how can you determine which layer (aqueous or organic) is on top, and which is on the bottom?
- Why Cyanide Is So Deadly?Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?What is the purpose of: A. The concentrated salt solution? B. adding isopropyl alcohol to the solution? Why does it need to be in low temperature?
- Is sodium propanoate (the sodium salt of propanoic acid) as effective a soap as sodium stearate (the sodium salt of stearic acid)? Explain.Part of the uniqueness of water as a solvent include exceptChlorophyll is the green pigment in the leaves of the plant, carotene imparts orange coloration while anthocyanin is known as the purple pigment. In one of their laboratory, a group was tasked to confirm the presence of these pigments on a certain leaf. After crushing several leaves, they soak it in isopropyl paper strip was dried and the group got the following measurements from the submerged tip of the paper strip: solvent font - 9.9cm, purple ink -8.6cm, yellowish orange ink - 6.4cm and green ink - 4.2 retention factor of each pigment. (d) Rank each pigment according to their solubility in the given solvent.