Why do we need to amplify the DNA at all in order to visualize it in a gel? Why run PCR?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Please number the bullets 

 

.
Why do we need to amplify the DNA at all in order to visualize it in a gel? Why run
PCR?
.
• Why do we need to include a search for the piece of DNA that codes for Tubulin if we
are really only interested in finding, in this case, a commonly used GMO sequence?
When we run that gel, we also run a ladder...why? What information does that give
us? Why does it form a ladder shape on the gel (what is happening to cause things to
separate out into many bands)?
.
Transcribed Image Text:. Why do we need to amplify the DNA at all in order to visualize it in a gel? Why run PCR? . • Why do we need to include a search for the piece of DNA that codes for Tubulin if we are really only interested in finding, in this case, a commonly used GMO sequence? When we run that gel, we also run a ladder...why? What information does that give us? Why does it form a ladder shape on the gel (what is happening to cause things to separate out into many bands)? .
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