Q: How do you make liquid bacteria culture?
A: It is important for growing more numbers of bacteria to isolate enough plasmid DNA for experimental…
Q: If the iodine step in a Gram-staining procedure was omitted, what color would you expect…
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: Why does a streaking method used to inoculate plates result in isolated colonies?
A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…
Q: What is the correct shape of the unknown bacterium growing on your MacConkey agar?
A: Macconkey Agar This agar includes bile salts, which are found in the gut. Bile salts help in…
Q: How does the heaviness of a bacterial smear affect its microscopic analysis?
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: What kind of medium might you make to selectively grow a bacterium that lives in your gut?
A: MacConkey agar is used as a selective and differential media for differentiating gut bacteria which…
Q: Is it acceptable to give a formal name to a microbe that hasn't been isolated and cultivated? What…
A: There are two main microbiology methods used for isolating bacteria from a clinical sample in order…
Q: Why is oil immersion necessary to see bacteria in the laboratory?
A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…
Q: A Gram stain indicates purple cells that are round and attached in chains. Which of the following…
A: Gram staining is a simple technique, in which two categories of bacteria are identified based on…
Q: Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the…
A: The term sterilization refers to any process that eliminates, kills, removes, or deactivates all…
Q: In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT:…
A: Organisms can be provisionally identified by growing them on medium and looking at their shape and…
Q: Why do you suppose endospore-forming bacteria are commonly found in the soil? Suppose your unknown…
A: Endospores are dormant, tough, vegetative structure produced by the bacteria from the Firmicute…
Q: What Are The Conditions When Gram Positive Bacteria Can Appear Gram Negative?
A: Gram positive bacteria have thick cell wall and they give positive result in gram stain test , in…
Q: citrobacter freundii
A: Citrobacter freundii is a species of anaerobic gram-negative bacteria which belongs to the family…
Q: What is culture media? Which culture media is used for gram negative bacteria and which one for gram…
A: Microbiology is the branch of science which deals with the study of different types microorganisms…
Q: You perform a Gram stain. Your Gram positive control, your Gram negative control, and your sample…
A: Gram-staining is a technique used to classify bacterial species, either as gram-positive or…
Q: Describe the bacterial colonies providing information on shape, color, size, elevation and edge…
A: *morphology of Escherichia coli is identified as rough or a smooth form. *The trough forms colonies…
Q: The rapid identification methods used in this activity are designed to identify __________.…
A: Bacteria are microscopic, single-celled, prokaryotic organisms. They are much smaller than other…
Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?
A: Culture media, also called as growth media, are particular combinations of nutrients and other…
Q: what is the gram staining procedure for e-coli
A: Gram staining is the laboratory technique which is used to differentiate between the morphology of…
Q: what is the culture media for gram-positive bacteria?
A: The culture media for gram positive bacteria is Mannitol salt agar . The presence of bile salts, as…
Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…
A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…
Q: Why did we both stab and streak our blood agar plates when inoculating them with bacteria?
A: Blood agar is a nutrient medium which contain essential nutrients for bacterial growth and blood of…
Q: List 2 reasons why a bacterium that should be gram positive might turn out gram negative.
A: Gram-positive and Gram-negative bacteria are differentiated by Gram staining.
Q: Streaking One drop of bacterial solution contains millions of cells. To identify a species,…
A: The purpose of streaking for isolation is to produce isolated colonies of an organism on an agar…
Q: When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal…
A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…
Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?
A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…
Q: You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look…
A: The gram staining procedure that is used for staining and visualizing the bacteria. The bacteria are…
Q: erial slide agglutination technique important in diagnostic procedure?
A: Immunoassays are bioanalytical ways that use the specificity of an antigen-antibody reaction to…
Q: How should agar plates be incubated? Why?
A: Incubating the plates to stimulate the growth of microbes is a crucial step in any microbiology…
Q: What technology is available for faster bacterial culture?
A: BASIC INFORMATION BACTERIA It is one of the type of biological cell. They belong to prokaryotic…
Q: What color would the bacterial cells have when viewed microscopically after the following treatment?…
A:
Q: What is the shape of bacteria under a microscope, what color is there, and is there Gram staining is…
A: Introduction- Gram staining is basic staining to identify the bacteria of medical significance. Gram…
Q: How does the bacterial cell structure contribute to the mechanism of Gram staining?
A: Bacteria are prokaryote organisms, that lack well-defined cell organeles and nuclei. Bacterial cell…
Q: what is the function of each one of the Gram-stain reagents?
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: What is the definition of Gram Positive?
A: When the stain combines with bacteria in a sample and the bacteria will either stay purple or turn…
Q: What is gram staining Step by step procedure Explain its basis and how it differentiate bacteria use…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: What color would the bacterial cells have when viewed microscopically after the following…
A: Gram staining is a technique proposed by Christian Gram to distinguish Gram positive and negative…
Q: Why can't gram staining be used for mycobacterium?
A: Gram staining is one of the staining techniques. This is usually used for differentiating between…
Q: Examine the image below. The bacteria in this image have been treated with gram straining procedure.…
A: The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring…
Q: What color would the bacterial cells have when viewed microscopically after the following treatment?…
A: Gram staining is a technique to distinguish between two broad types of bacterias on the basis of…
Q: identifying the type of bacteria I have cultured. I know that it is gram-negative and cocci in…
A: There is only two type of bacterias which do not grow so well on EMB medium. 1. Shigella 2.…
Q: Which bacterial type ( Gram positive or Gram negative) is more sensitive to the antibiotic…
A: Penicillin is the group of antibiotics which were originally derived from the penicillium molds. It…
Q: Why does nigrosin not stain the bacteria?
A: Nigrosin is an acidic stain, used in the negative staining technique. Staining is a part of…
Q: What can happen to cause gram-positive bacteria to appear gram-negative?
A: There are numerous methods of characterizing bacteria. One strategy depends on the cell membrane. In…
Q: You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix.…
A: In serial dilutions, dilutions are multiplied at each step. We can calculate dilution by dividing…
Q: Are there any chemical differences between the cell walls of gram-positive and negative bacteria…
A: Bacteria are the prokaryotes which fall under Kingdom Monera. They can be found everywhere. They…
Q: A microbiologist is working with a pure strain of bacteria isolated from a mixed sample. Which of…
A: Introduction Helicobacter pylori, formerly known as Campylobacter pylori, is a spiral (helical)…
Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?
A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…
- Why is it important to follow sterile technique?
- What can happen to cause gram-positive bacteria to appear gram-negative?
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- How does the growth rate of a specific bacterium impact how easy or difficult it is to treat clinically?Why is oil immersion necessary to see bacteria in the laboratory?Is it acceptable to give a formal name to a microbe that hasn't been isolated and cultivated? What type of name should a microorganism have if it has been well identified but cannot yet be cultured in isolation?
- When do we make the determination that a bacterium is Gram positive? A) Safranin washes the crystal violet die out of the cell wall. B) Crystal violet die is not able to absorb into the cell wall. C) Alcohol does not wash the crystal violet die out of the cell wall. D) Crystal violet turns the bacterium a yellow color.Why should bacterial culture plates be incubated inverted (bottom up)?You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? a)red/pink b)purple c)colorless
- After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?N Please observe the following cells that have been Gram stained. "* 3 E ER $ JO 4 R % 5 6 F G & Y I U 8 00What can happen to cause gram-positive bacteria to appear gram-negative?