Why is the improved synthesis of nucleotide triphosphates more environmentally benign? Check all that apply. reducing the chance for reacting molecules to collide eliminates the need for polymerase chain reaction making use out of a waste product (glycerol) removal of a hazardous solvent to lower waste and cost
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- Why is agarose an ideal gel matrix for electrophoresis experiments? Because it forms a liquid at all temperatures. Because it is un-ionized and uncharged Because it is positively charged fs f6 f7 fg fg f1o $ [8 19 4 6 7 8. 4. 5 R Y F G J 2 K エNO Which nucleotide is shown in the picture above O deoxyguanosine triphosphate guanosine triphosphate savedModify methionine to show its zwitterion form. Select Draw с N 0 Templates S H More 1C || H0 C 1 с | CH₂ | CH₂ | S 1 CH3 HIN I N-H Erase Q 2 Q
- Among the affinity chromatography resins below, select the one which purifies proteins that have been engineered with a Histidine-Tag at either the N- or the C-terminus Group of answer choices Protein A Protein G Avidin NickelWhat is the role of his 12 in the RNase-catalyzed hydrolysis of RNA, as indicated in the Figure below? 5' His 119 RNA CH₂ H H 3 O Base 1' 12' 8-H His 12 -P-0-CH₂ a Base H H O -0-P=O H OH NH It acts as a general base, abstracting a proton from the 2' hydroxyl in order to increase its nucleophilicity. It forms an H-bond with his119 in order to stabilize the transition state. It donates a proton to improve the quality of the leaving group. It works through concerted general acid-base catalysis with his119 in order to favour the products of the reaction. Two of the above are true.NH2 но ÓH ÓH OH Он Which nucleotide is shown in the picture above? deoxyadenosine triphosphate adenosine triphosphate
- using cysteine, serine, and aspartic acid create a tripeptide by performing 2 dehydration synthesis reactionsRNA Ribonucleotide monophosphates (Pick one purine and one pyrimidine) PART B. Building Polymers- Label which is the purine and which is the pyrimidine. Circle the sugar on each.To make Cytidine 5'-triphosphate we must ALVWAYS first make which nucleotide? Adenylate Thymidylate Inosinate O Xanthylate O Uridylate Guanylate
- H. HD N. A N- H- H. 0=P-0- CH2 H. H. H. OH) H B. Where would this nucleotide hydrogen-bond to its complementary base pair? A エProflavine was used to treat wounded soldiers in the Far East during the second world war. Which of the following statements is false regarding proflavine! H₂N7 NH₂ Its target is located in the cell membrane. Its planar shape is important to intercalation. The primary amine groups are protonated and interact with phosphate groups of the DNA backbone. When inserted into DNA, the tricyclic system can form van der Waals interactions with base pairs above and below it.The nucleotides were chemically modified. Select the modification that will result in a nucleotide being unable to link to the new nucleotide during the nucleic acid synthesis. 1 Adding a hydroxyl group to the second carbon in a sugar 2 Removing a hydroxyl group from the third carbon in a sugar 3 Both modifications will make the nucleotide become functional in the synthesis of nucleic acids.