You are planning an experiment to use zinc finger nucleases to generate a double-stranded DNA break in a targeted sequence of DNA Name the protein domain in a zinc finger nuclease that is responsible for generating the DNA break Fok1 Name two other genome-engineering proteins that could be used for generating the double-stranded DNA break   What are the advantages and disadvantages of each strategy?

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter19: Genomes And Proteomes
Section: Chapter Questions
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Problem 4: 

You are planning an experiment to use zinc finger nucleases to generate a double-stranded DNA break in a targeted sequence of DNA

  • Name the protein domain in a zinc finger nuclease that is responsible for generating the DNA break
    1. Fok1
  • Name two other genome-engineering proteins that could be used for generating the double-stranded DNA break
  •  
  • What are the advantages and disadvantages of each strategy?
Problem 4:
You are planning an experiment to use zinc finger nucleases to generate a double-stranded
DNA break in a targeted sequence of DNA
(a) Name the protein domain in a zinc finger nuclease that is responsible for generating the
DNA break
(b) Name two other genome-engineering proteins that could be used for generating the
double-stranded DNA break
(c) What are the advantages and disadvantages of each strategy?
Transcribed Image Text:Problem 4: You are planning an experiment to use zinc finger nucleases to generate a double-stranded DNA break in a targeted sequence of DNA (a) Name the protein domain in a zinc finger nuclease that is responsible for generating the DNA break (b) Name two other genome-engineering proteins that could be used for generating the double-stranded DNA break (c) What are the advantages and disadvantages of each strategy?
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