You discover a microbe that you believe is the causative agent of a new disease. Using Koch's postulates as a guide, describe the experiments that you would perform to confirm that the microbe causes the disease.
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- The first step for directly linking a microbe to a specific disease according to Koch's postulates is to O isolate microbes from the blood of healthy animals. obtain a sample of blood or other body fluid from a diseased animal. O culture the blood or other body fluid from a discased animal using nutrient medium. O inject a sample of blood or other body fluid from a diseased animal into a healthy animal. O compare the blood of a sick animal to blood obtained from a healthy animal.List the main features of Koch’s postulates and explain why it is sodifficult to prove them for some diseases.The following are the limitations of Koch's postulates EXCEPT: A. some pathogens cannot grow on artificial media and therefore cannot be identified as the causative agent of the disease B. some diseases involve multiple pathogens which produce similar symptoms making it difficult to pinpoint the causative agent C. some diseases are host-specific and re-inoculation may pose ethical concerns D. some microorganisms are present in the body fluids of the infected animal which make them difficult to be cultured
- What is one situation where Koch’s postulates are impossible to complete? In other words, what factors about a microbe or factors about a disease make it impossible for Koch's postulate to be performed with that microbe or disease?Which of the following is not a condition of Koch’s postulates?a. isolate the causative agent of a diseaseb. cultivate the microbe in a labc. inoculate a test animal to observe the diseased. test the effects of a pathogen on humansWhy are Koch’s postulates not sufficient to establish the cause of all infectious diseases?
- Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?Attached below is the study about the effects of handwashing and the effectiveness of soap against bacteria. What conclusions can be generated from this result? A C before washing hands 10 second hand wash B D hot water only rinse 30 second hand wash Figure 8: Agar plates with bacteria A) before washing hands B) after rinsing hands in hot water C) After washing hands with soap for 10 seconds and D) after washing hands for 30 seconds. Images show that the number of bacteria on the plate are not greatly reduced after a hot water rinse or 10 second handwash but bacterial levels are reduced after a proper hand wash for 30 seconds.
- Put Koch’s postulates in order.(a) The disease organism must be isolated in pure culture.(b) The disease organism must be recovered from the in-oculated animal.(c) The specific causative agent must be found in every caseof the disease.(d) Inoculation of a sample of the culture into a healthy, sus-ceptible animal must produce the same disease.Explain Koch’s Postulates and the proof of pathogenicity.The first step for directly linking a microbe to a specific disease according to Koch's postulates is to A.isolate microbes from the blood of healthy animals. B. culture the blood or other body fluid from a diseased animal using nutrient medium. C. inject a sample of blood or other body fluid from a diseased animal into a healthy animal. D. obtain a sample of blood or other body fluid from a diseased animal.