You have a 12 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 8.14. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 6.55. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 12 mL sample. Acetycholine in original sample:_________ nmol

General Chemistry - Standalone book (MindTap Course List)
11th Edition
ISBN:9781305580343
Author:Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Publisher:Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Chapter16: Acid-base Equilibria
Section: Chapter Questions
Problem 16.12QP: The pH of 0.10 M CH3NH2 (methylamine) is 11.8. When the chloride salt of methylamine, CH3NH3Cl, is...
icon
Related questions
icon
Concept explainers
Question
You have a 12 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH
of 8.14. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine
to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the
incubation period, you measure the pH again and find that it has decreased to 6.55. Assuming there was
no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 12 mL
sample.
Acetycholine in original sample:________ nmol
Transcribed Image Text:You have a 12 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 8.14. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 6.55. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 12 mL sample. Acetycholine in original sample:________ nmol
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 5 steps with 3 images

Blurred answer
Knowledge Booster
Ionic Equilibrium
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
General Chemistry - Standalone book (MindTap Cour…
General Chemistry - Standalone book (MindTap Cour…
Chemistry
ISBN:
9781305580343
Author:
Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Publisher:
Cengage Learning
Chemistry: Principles and Practice
Chemistry: Principles and Practice
Chemistry
ISBN:
9780534420123
Author:
Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Publisher:
Cengage Learning
Fundamentals Of Analytical Chemistry
Fundamentals Of Analytical Chemistry
Chemistry
ISBN:
9781285640686
Author:
Skoog
Publisher:
Cengage
Introductory Chemistry For Today
Introductory Chemistry For Today
Chemistry
ISBN:
9781285644561
Author:
Seager
Publisher:
Cengage
Principles of Modern Chemistry
Principles of Modern Chemistry
Chemistry
ISBN:
9781305079113
Author:
David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:
Cengage Learning