2 2. You have the following polypeptides at pH 7.25 and are attempting to separate themusing an anion exchange column.1. GHYSDE2. СКОKRA3. PMDGVLChoose the most likely elution order below. Circle your answer.Aelution volumeelution volumeelution volumeelution volumeTproteinIprotein)

For an amino acid residue, If pKa > pH, the ionizable group remains in its protonated state.…

You have the following polypeptides at pH 7.25 and are attempting to separate them using an anion exchange column. 1. GHYSDE 2. CKQKRA 3. PMDGVL Choose the most likely elution order below. Circle your answer.

You have the following polypeptides at pH 7.25 and are attempting to separate them using an anion exchange column. 1. GHYSDE 2. CKQKRA 3. PMDGVL Choose the most likely elution order below. Circle your answer.

Chemistry: Principles and Reactions

8th Edition

ISBN:9781305079373

Author:William L. Masterton, Cecile N. Hurley

Publisher:William L. Masterton, Cecile N. Hurley

Chapter23: Organic Polymers, Natural And Synthetic

Section: Chapter Questions

Problem 44QAP: A 1.00-mg sample of a pure protein yielded on hydrolysis 0.0165 mg of leucine and 0.0248 mg of...

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Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

2. You have the following polypeptides at pH 7.25 and are attempting to separate them
using an anion exchange column.
1. GHYSDE
2. СКОKRA
3. PMDGVL
Choose the most likely elution order below. Circle your answer.
A
elution volume
elution volume
elution volume
elution volume
Tprotein
Iprotein)

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