You will be given the plaque assay plates to analyse from three different bacteriophages stocks You need to determine which bacteriophage should be used in subsequent transductions. You should give a reason why. PHYSIOLOGY AN GENETICS I Experimental set up: all culture plates were incubated at 37°C over night Bacteriophage A 200 μl of bacteriophage A was added to 800 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-4. 100 μl of the mixed culture was then added to 9.9 ml molten agar and poured into the petri dish. Bacteriophage B 100 μl of bacteriophage B was added to 900 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-5. 1000 ul of the mixed culture was then added to 9 ml molten agar and poured into the petri dish. Bacteriophage C 250 μl of bacteriophage C was added to 750 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-6. 1000 μl of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.
You will be given the plaque assay plates to analyse from three different bacteriophages stocks You need to determine which bacteriophage should be used in subsequent transductions. You should give a reason why. PHYSIOLOGY AN GENETICS I Experimental set up: all culture plates were incubated at 37°C over night Bacteriophage A 200 μl of bacteriophage A was added to 800 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-4. 100 μl of the mixed culture was then added to 9.9 ml molten agar and poured into the petri dish. Bacteriophage B 100 μl of bacteriophage B was added to 900 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-5. 1000 ul of the mixed culture was then added to 9 ml molten agar and poured into the petri dish. Bacteriophage C 250 μl of bacteriophage C was added to 750 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-6. 1000 μl of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter17: Bacterial And Viral Genetics
Section: Chapter Questions
Problem 1ITD
Related questions
Question
Bacteriophage A average = 136 plaques
Bacteriophage B average = 38 plaques
Bacteriophage C average = 27 plaques
Please help find the PFU/ml and answer the question on the best bacteriophage. Thanks
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