Your lab partner set up plate 4 by spreading a pure culture of E. coli onto the plate and addind antibiotic discs. Is this a reasonable result? Explain the result and give your reasoning.
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Your lab partner set up plate 4 by spreading a pure culture of E. coli onto the plate and addind antibiotic discs. Is this a reasonable result? Explain the result and give your reasoning.
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- Repeat the procedure to collect data on the plates containing E. coli (Figure 4). Record the results in table 2. 5 7 2 6 7 5 Figure 4 Two replicates of Escherichia coli treated with six antimicrobial agents plus a controlYou mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do not know if you grew staph epidermis or E. coli. You found that the organism growing on the mannitol salt agar remained red after incubation. It is most likely that the organism is E.coli. a) True b) FalseYou are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?
- Suppose your Professor handed you a test tube with 2.0mL of E.coli broth culture and told you to make a 10-1 dilution of the ENTIRE culture. Explain how you would do this.you are given a mix culture of S aureus, E. coli and P. aeruginosa. besides using the streak plate method what other method could you choose to separate the bacteria? the result you will be looking for and interprete the result.You are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method what other methods could you use to separate the bacteria? Please state what method(s) you would use, the result you would be looking for to help identify each bacterium and the interpretation of the result.
- Can you say that the microbial composition changes over time in each of the body locations? Give two pieces of evidence. Why do you think the scientists also tested the soil around the cadaver? The study was done with mice and their cadavers decomposed on soil graves in the University of Colorado Transgenic Facility. Do you think the graphs would have looked different if the decomposition events would have happened outside during the hot summer months? Explain.There are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2Samples from an ill patient were collected by a physician for testing. A bacterial infection is suspected. Using the unknown flowcharts, a microbiologist conducted various tests to determine the identity of the unknown bacterial isolate. The resulting data is included in this presentation. Identify each test.
- Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture into a 9 ml LB medium. After this, from 10-4 and 10-5 dilution tubes 100µl were plated onto LB agar plates. Upon overnight incubation at 37°C, 200 colonies were counted in 10-4 and 22 colonies were present on 10-5 plates. How many colony-forming units were present per ml of the original culture? If the formula for CFU/ml =no. Of colonies/dilution factor*volume of culture plateThe student advises using the same selection procedures and inoculating a 5 mL overnight culture with the transformed cells instead of searching for transformed coli on LB agar plates in order to save time. Why isn't this a wise decision?