Z-germicSide is an antimicrobial agent. Based on the given data set below, describe how effective Z-germicSide is as a disinfectant. Name of disinfectant Z-germicSide Phenol KEY: No growth, += Growth Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 Growth after 5 mins + + + + + + Growth after 10 minutes + + + Growth after 15 mins + +
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- With the aid of diagrams, illustrate the biochemical basis of the following: Allopurinol used in the management of gout Hydroxyurea as an antineoplastic agent 6-mercaptopurine, an anticancer agent Trimethoprim, an antibacterial agentThe type of antimicrobial drug that would be most likely to have toxic effects in humans based on the premise of selective toxicity when treating microbial infection would be a drug that O 1) inhibits the synthesis of the cell wall O 2) inhibits metabolic pathways O 3) disrupts the cytoplasmic membrane 4) inhibits protein synthesis 5) All of the above have equal potential to be toxicessential oils have been studied as potential antimicrobial agents. These naturally occuring compounds are linked to having the following type of activity in clinical trials: virucidal, septicidal, bactricidal, fungicidal
- Choose one specific way from each of the two methods of inhibiting the growth of microbes below. Describe the method, explain the mechanism of action of killing the microorganisms and give practical applications of your chosen ways. 1. Physical Methods - Heat, cold, dessication, radiation, ultrasonic waves, filtration, gaseous atmosphere 2. Chemical Methods- disinfectants, antisepticsBriefly explain this statement "Antineoplastic natural products- Taxol".please explain at your own words.Some bacteria produce the enzyme penicillinase, which breaks the beta-lactam ring in the penicillin molecule and converts it into penicilloic acid, which is harmless to bacteria. This is an example of which of the following mechanisms of antibiotic resistance? which option below is the answer? Destruction or inactivation of the antibiotic by the microbe Preventing penetration to the target site within the microbe Alteration of the drug’s target site(s) within the microbe None of the other four answers are correct Rapid ejection (pumping) of the drug out of the microbial cell
- If you have 4 compounds and you test them for antimicrobial activity using a disk diffusion assay and one is very active, one is moderately active, one is slightly active and one is not active at all, draw the disk, the zone of inhibition that you would see and a pretend numerical value to indicate the size of the zone in mm, give each one a number 1 – 4 and rank them from most effective to least effective as an antimicrobial.According to USP Chapter <797>, allergenic extract preparation can be made in a doctor’s office facility by trained personnel only if the allergenic extract preparation: a)Is compounded under a laminar air flow hood Is compounded by a pharmacist b)Contains an antimicrobial preservative c)Is used immediately after preparation d)Is prepared only from plant origin materialChoose the false statement below. O Effective antimicrobials may not be used commercially because of their mode of delivery or the time it takes them to leave the body. O Decontamination of an object is not necessary if disinfection is done properly. O In general, acid fast bacteria are less sensitive to antimicrobials than are Gram-negative bacteria or fungi. Question 8 Choose the false statement below: O Using three different classes of antimicrobials in combination greatly decreases the chance of developing resistance because the drug resistance strategy for one is the same for others. O Drug resistance to antimicrobials has proven to be extensive against chemotherapeutics as well as other chemical antimicrobials. O Collateral damage is a term that refers to the killing of normal body flora as well as pathogenic bacteria when taking antimicrobials or antibiotics.
- An organism produced the following growth pattern when grown at different pH's. The best description for this organism is: Key: 0 = no growth + = light growth ++ = medium growth +++ = heavy growth pH 1 3 5 7 9 11 Growth 0 0 + + ++ +++ Group of answer choices Alkaline-tolerant neutrophile Obligate alkalinophile Acid-tolerant neutrophile Neutrophile Acid-tolerant alkalinophileIdentify two factors that make biofilms more tolerant to antimicrobials and make sure to include their mode of action.N. benthamiana will be infiltrated with a solution containing OD600=0.3 of each experimental Agro containing construct and OD600=0.1 of p19. Calculate the volume of cultures (V construct; V p19) needed according to the formulas: V construct = V final × 0.3/OD600; V p19 = V final × 0.1/OD600. One mL of infiltrate is often enough to complete a small experiment. How to plan your final volume accordingly?