Which of the following is/are not required for DNAreplication to occur?a. DNA polymerase d. primersb. nucleotides e. helicasec. template DNA f. all are required
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Which of the following is/are not required for DNA
replication to occur?
a. DNA polymerase d. primers
b.
c. template DNA f. all are required
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- Which of the following best describes the process of DNA seqencing. a. DNA is seperated on a gel and the different bands are labled with flouroscent nucleotides and scanned with a laser. b. A laser is used to flurorescently label the nucleotides present with in the DNA , the DNA is run on a gel and then the DNA is droken into fragments c. Nucleotides are scanned with a laser and incrprorated into the DNA that has been seperated on a gel and then DNA is amplified with PCR. d. fragments of DNA are produced in a reaction that lables them with any of four different fluroscent dyes and the fragmented then are run on a gel and scanned with laser e. DNA is broken down into its constituents nucleotides and the nucleotides are then run on a gel and purified with a laserThe diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction? Group of answer choices a. The number of copies triples (or triplicates). b. The number of copies does not change. c. The number of copies quadruples (or quadruplicates). d. The number of copies doubles (or duplicates). e. The number of copies halves.Which of the following reagents is NOT correct reagents for the Sanger method of DNA sequencing? a. All four dNTPs b. a small amount of specific ddNTP c. DNA Polymerase d. DNA template to be sequenced e. Pair of primers
- Which of the following is not essential to carry out the polymerasechain reaction?a. primers b. DNA polymerase c. gel electrophoresis d. high temperatureIn E.coli, the following enzymes do NOT synthesize the RNA primer for Okazaki fragments? A.DnaA B. DnaC C.DnaG D.None of the aboveDefine the following terms: a. DNA typing b. short tandem repeats c. DNA profile d. nucleosome e. retrotransposon
- The polymerase chain reaction requires the following except A. primers complementary to the ends of the sequence to be amplified B. carefully controlled temperature conditions C. cycles of denaturation, annealing, and extension D. RNA polymerase to synthesize RNA primersDuring a PCR reaction, all the following events occur except: A. Hybridization B. Binding of a 3’OH group in the active site of DNA polymerase C. Addition of nucleotide monomers on the 3’ end of the template DNA D. DNA denaturation E. Semi conservative replicationWhich of the following chemicals or procedures is not involved in a typical Polymerase Chain Reaction (PCR). Select one: O a. ddNTPs b. O C. O d. Oe. O f. Repeated cycling (typically 32 cycles) for exponential target amplification DNA strand replication/extension Annealing of oligonucleotide primer or primers Thermostable (Taq) DNA polymerase Buffer with required cofactor: Mg 2+ Og. DNA template Oh. High temperature denaturation O i. dNTPs
- Which of the following statements is TRUE concerning the synthesis of the leading and lagging strands of DNA in prokaryotic cells? a. O b. The leading strand is synthesized by one polymerase III continuously, and the lagging strand is synthesized by several molecules of DNA polymerase III. d. The leading and lagging strands are synthesized at the same time by the one DNA polymerase I. O c. The leading and lagging strands are synthesized at the same time by the one DNA polymerase III. The leading strand is synthesized by one polymerase III, and the lagging strand is synthesized by DNA polymerase I.1. A.) Deletion mutation is a loss of a single base by damage. B.) Point mutation is when a nucleotide is paired with a wrong nucleotide during RNA replication. a. Statement A is correct b. Statement B is correct c. Both A and B are correct d. Both A and B are incorrect Answer: 2. This is a process that produces a change in the structure of the DNA by forming adenine rather than guanine. a. Reverse transcription of guanine b. Alkylation of the 06-Guanine c. Reverse translation of guanine d. Methylation of guanine bases Answer: 3. This type of DNA repair happens when you are removing a single damaged base of DNA strand. a. Nucleotide repair b. Base excision repair c. Strand break repair d. Base nucleotide replacement Answer:Which statement about Okazaki fragments is true? Select one: a. DNA polymerase doesn’t need a primer to build these fragments b. They act as a primer that initiates DNA replication. c. They correct errors made during earlier phases of DNA replication. d. They are necessary because DNA polymerase can only build DNA in the 5’ to 3’ direction, so for one of the strands at each fork, the DNA polymerase can only buildaway from the fork. e. They prevent the ends of chromosomes from shortening with every replication.