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12/26/23, 3:26 PM 12.1 PCR Review Questions: 23F-LIFESCI-7A-LEC-1 Cell and Molecular Biology (cross-listed) https://bruinlearn.ucla.edu/courses/170946/quizzes/983732?module_item_id=6211396 1/4 12.1 PCR Review Questions Due Nov 30 at 2pm Points 5 Questions 5 Time Limit None Allowed Attempts 2 Attempt History Attempt Time Score KEPT Attempt 2 2 minutes 5 out of 5 LATEST Attempt 2 2 minutes 5 out of 5 Attempt 1 2 minutes 4 out of 5 Score for this attempt: 5 out of 5 Submitted Nov 30 at 1:47am This attempt took 2 minutes. 1 / 1 pts Question 1 Select the correct order of steps during RCR. denaturation, annealing, extension Correct! Correct! annealing, initiation, termination denaturation, extension, termination initiation, extension, termination initiation, denaturation, extension 1 / 1 pts Question 2
12/26/23, 3:26 PM 12.1 PCR Review Questions: 23F-LIFESCI-7A-LEC-1 Cell and Molecular Biology (cross-listed) https://bruinlearn.ucla.edu/courses/170946/quizzes/983732?module_item_id=6211396 2/4 What is the benefit of using Taq polymerase in PCR? Because it is taken from bacteria that live in high temperatures, it stays active during denaturation steps of the reaction. Correct! Correct! Because it is taken from bacteria, this enzyme works much more efficiently than other types of DNA polymerase. Because it is taken from bacteria that live in high temperatures, it does not have a proofreading function. None of the other answer options is correct. Because it is taken from bacteria, it makes fewer mistakes. 1 / 1 pts Question 3 The polymerase chain reaction (PCR) is used to generate: a single copy of a targeted region of DNA. multiple copies of a targeted region of DNA. Correct! Correct! single copies of whole chromosomes. multiple copies of whole chromosomes and a targeted region of DNA. multiple copies of whole chromosomes.
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