Spectrophotometry Lab (2) (1)
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Dawson College *
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Subject
Chemistry
Date
May 17, 2024
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6
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Dawson College
Department of Chemistry & Chemical Technology
Laboratory Report
Chemistry of Solutions
Experiment # 3
Spectrophotometry
Written by:
Natalie Kasnakian
2338634
Partner: Natalya Babkine
Presented to: Ms. Wanying Zhang
Date of the Experiment: 08/03/2024
Date of the Lab Report Submission: 15/03/2024
I.
Data and Results
Concentration of the stock solution, M
0.020 M
Concentration of the primary solution, M
8.0 x 10^-4 M
Concentration of the solution 1, M
4.0 x 10^-4 M
Concentration of the solution 2, M
2.7 x 10^-4 M
Concentration of the solution 3, M
2.0 x 10^-4 M
Concentration of the solution 4, M
1.6 x 10^-4 M
Concentration of the solution 5, M
1.1 x 10^-4 M
Unknown number:
#5
Absorbance of the unknown:
0.171
Concentration of the unknown (from graph), M
1.9 x 10^-4 M
II.
Sample Calculations
Calculations of the Concentrations of the
Solutions:
??𝑛𝑂
4
1.
Concentration of the Primary
(Dilution equation)
?
1
𝑉
1
= ?
2
𝑉
2
10.00 mL = 0.01000 L
(0. 020 ?)(0. 01000 ?)
= (?
2
)(0. 2500 ?)
(?
2
) =
(0.020 ?)(0.01000 ?)
(0.2500 ?)
(?
2
) = 8. 0 × 10
−4 ?
2.
Concentration of Solution 1
(Dilution equation)
?
1
𝑉
1
= ?
2
𝑉
2
10.00 mL = 0.01000 L
(8. 0 × 10
−4 ?)(0. 01000 ?)
= (?
2
)(0. 02000 ?)
(?
2
) =
(8.0×10
−4 ?)(0.01000 ?)
(0.02000 ?)
(?
2
) = 4. 0 × 10
−4 ?
(The method of calculation for the molar concentration of solution 1 is the same for solutions 2,
3, 4 and 5)
III.
Graphs
1.
Absorbance vs. Molarity of Potassium permanganate
IV.
References
1.
Chemistry of Solutions Lab Manual
Department of Chemistry. (2024). Chemistry of Solutions: Laboratory experiments (Experiment
#3, Spectrophotometry).
Dawson College.
Montreal. p 19-23
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Related Questions
NAME:
LAB SECTION:
EXPERIMENT 21: Spectroscopy of Dyes
POSTLAB EXERCISE
1. AUV/V is a spectrometer used to measure the absorbance of a solution of hemoglobin bound to oxy-
gen at 590 nm. The molar absorptivity was determined to be 8.57 × 104 M-'cm-. The cuvette used
had a 1.00-cm sample pathlength. The measured absorbance was ), 140. What was the concentra-
tion of hemoglobin in the solution?
2. What would be the absorbance of the solution in question 1 if the pathlength were 2.00 cm?
nohntooote
196
arrow_forward
Question 19 of 27
>
Graphs are a common data analysis tool used in all branches of science. The given graph represents how light absorbance at
423 nm varies with concentration of an organic dye. Identify the parts of the graph.
Answer Bank
y = 0.210x – 0.032
line equation
Title
Abs423
Dye Conc. (nM)
best-fit line
0 i 2 3 4 5 6 7 8 9 10
arrow_forward
Timer
Course Contents » ... » Data, Statistics, and Graphing » Graphing Absorbance (Optical Density) versus Molar Concentration
The following table contains some data showing the effect of molar concentration of a solution on the absorbance (optical density) reading from a spectrophotometer.
No
↑ Does the solution in your graph follow Beer's Law?
Concentration (M)
Submit Answer Incorrect. Tries 37/99 Previous Tries
0.13
0.28
0.43
0.58
0.73
0.88
1.03
1.18
1.33
1.48
What is the concentration of a solution that has an Optical Density of 0.21? (Give units in your answer).
0.21M
You are correct.
Your receipt no. is 155-7520 ?
What is the concentration of a solution that has an Optical Density of 0.91? (Give units in your answer).
0.81 M
Previous Tries
Optical Density
To assist in graphing, right click on this excel spreadsheet file, save it to your computer, and open it in Excel. Enter the above data in the appropriate spaces in the spreadsheet. Select the graph that is created, and
print…
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Chemistry
Instrumental Analysis Class
The experiment is OES: Cleaning Validation of removal of pharmaceutical drugs and calibration standards by fluoresence spectroscopy
- Why the instrument use two detectors and monochromators? What type of monochromators are used in our instrument?
- Do you think that a similar experiment can be prepared for the analysis of naproxen and benzoic acid? Explain.
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In the interference experiment preformed, sodium ascorbate powder was added to water to prepare the stock concentration of the interfering substance. On the package label, the manufacturer states that every 1 gram (1,000mg) of sodium ascorbate powder contains 120mg of sodium and 880mg of ascorbate (vitamin C).
How much sodium ascorbate must be weighed out (in milligrams) and added to 100 mL of water in order to have a final stock concentration of pure ascorbate equal to 250 mg/dL?
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B. Quantitative Spectroscopy
Prepare two graphs, %T vs. Concentration and Absorbance vs. Concentration, using the data for the cobalt standards. The concentrations of Cobalt standards 2-4 can be calculated using simple dilution calculations. Concentration should be on the x-axis for each of these graphs. The first should clearly show some curvature while the second should be a linear curve.
Using the absorbance graph you can determine the concentration of the unknown. Take the absorbance value for the unknown and find that value on the y-axis. Draw a horizontal line from that point on the y-axis to the plotted line. From the point where the horizontal line intersects the plotted line, draw a vertical line straight down to the x-axis. Record the value from the point where you hit the x-axis as the unknown concentration.
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QUALITATIVE DETECTION OF ORGANIC COMPOUNDS
Would you say that all organic compounds give evidence of containing carbon when they are burned or heated?
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How does a CEM detector work?
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Which part of the instrument perform the given function
introduction of a micro amount of sample
converts sample components into gaseous ions
orts the ions according to m/z
converts m/z values to electrical signals
converts signal/results into a mass spectrum
A.
mass analyzer
B.
Vacuum system
C.
Ion source
D.
Processor
E.
inlet system
F.
detectors
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chem 132 Exam 1 2022 • Saved -
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23. Which of the following compounds contain a hemiacetal functional group?
OCH3
OH
но.
OCH3
A
OCH3
В
OCH3
C
a. A and B b. B and C
c. B only
d. C only
24. Which of the following compounds contains an acetal functional group?
OCH3
-OCH3
-OH
OCH3
A
B
a. A and B
c. B and C
b. A and C
d. C only
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PRE-LAB QUESTIONS
Experiment 121
Complete these questions prior to attending lab. Some of the results will be useful in conducting
the experiment, so you should record those results in the appropriate section of the lab as well.
1. In Part A, step 1, you are directed to set up the
spectrophotometer for measurement of the
FeSCN2+ product ion.
a. Based on the spectra given here, what
would be an appropriate wavelength for the
measurement? Review Appendix I if
necessary to again familiarize yourself with
the issues related to this decision.
b. Briefly explain your choice.
0.5
A 0.4
0.3
Absorbance A
Absorbance spectra for Fe³+ and FeSCN²+
0.2
0.1
0
400
0.02 M Fe³+ (dissolved in HNO3)
E121-11
0.000048 M FeSCN²+
500
600
wavelength (nm)
700
2. In Part A, step 4, you will experimentally determine the value of the molar absorptivity, &, for
FeSCN2+ at your selected wavelength. Using the Beer-Lambert law, calculate an approximate
value for & based on the spectrum of 0.000048 M FeSCN2+ provided…
arrow_forward
100
MASS SPECTRUM
80
60
40
20
0.0
10
20
30
40
50
60
m/z
NIST Chemistry WebBook (https://webbook.nist.gov/chemistry)
1. What is the M'?
2. What is the m/z of the base peak?
3. Does it contain chlorine? Explain your answer.
Rel. Intensity
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Unknown sample
Trial 1
Trial 2
Trial 3
Absorbance (AU)
0.388
0.389
0.388
What is the Corrected Absorbance of each trial? How do you get the corrected absorbance?
arrow_forward
To determine the amount of copper and the identity of the copper compound in the unknown sample, a 100-mL solution containing 120.8 mg of the sample was initially prepared. Then, 5.00 mL of this solution was pipetted and diluted with deionized water in another 100-mL volumetric flask. The absorbance of this diluted solution was determined three times at the analytical wavelength. The data is in the table below.
Unknown sample
Trial 1
Trial 2
Trial 3
Absorbance (AU)
0.375
0.374
0.376
What is the concentrartion of Cu in the analyzed solution?
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A sample solution containing quinine was analysed by fluorescence spectroscopy.
5 mL of the sample solution was diluted with 0.1 M HCl to a final volume of 100 mL. The fluorescence of the solution was measured and gave a value of 54. A reference solution containing 0.086 mg/mL of quinine gave a fluorescence reading of 38. A blank solution gave a fluorescence reading of 21.
Calculate the quinine content of the sample solution in mg/mL.
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Copy o
B S2 Perfo
Student
Indeper
B Quizzes X
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mochemistry Exam
Angel Licona Rivera: Attempt 1
A 71.0 gram gold bar was heated in an experiment and 3,000 J of energy was
transferred to the bar. What was the change in temperature for the bar in °C?
Specific Heat: (CGold =0.126 J/g°C)
Your Answer:
Answer
units
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To determine the amount of copper and the identity of the copper compound in the unknown sample, a 100-mL solution containing 120.8 mg of the sample was initially prepared. Then, 5.00 mL of this solution was pipetted and diluted with deionized water in another 100-mL volumetric flask. The absorbance of this diluted solution was determined three times at the analytical wavelength. The data is in the table below.
Unknown sample
Trial 1
Trial 2
Trial 3
Absorbance (AU)
0.388
0.389
0.388
What is the concentration of Cu in analyzed solution (mg/L) for each trial?
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In the HQS-Mg fluorescence part of Lab 6, a calibration curve is made of fluorescence intensity vs.
MgCl2 concentration (M). The standard solutions for the calibration curve were made by mixing a
small amount of 0.01 M MgCl2 with some HQS solution. Calculate the concentration of MgCl2 (M)
in each of the following standards. Report your concentrations to 3 sig figs.
Fluorescence
Volume of 0.01 M
Volume of 1 mM
Concentration of MgCl2 (M)
Intensity
MgCl2 (mL)
HQS (mL)
0.031250902
0.8
100
0.049490754
0.8
50
0.059803507
2
100
0.085834029
3.6
100
Hint: The total volume of each solution is volume of 0.01 M MgCl2 + volume of 1 mM HQS.
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A student measures the absorbance of a solution in lab a total 4 times and obtained the following results:0.219, 0.224, 0.206, 0.223a. Using the Grubbs test, decide whether any values should be rejected at the 95% confidence level.b. What should the student report as the 95% confidence interval for the absorbance of the solution?
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Document1 - Word
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Write the corresponding letters to the following descriptions. Each proposal can be associated with 0 or
1 match and each letter is not necessarily associated with a proposal.
a. [Ar] 4s?
b. 1s 2s2p63s23p3
c. 1s2s2p2
d. [Ne] 3s23ps
e. [Ar] 4s23d10
f. 1s22s!
g. None of those answers
1. I am a halogen
2. I am a transition metal
3. I am alkaline
4. I have exactly 2 single electrons
5. I am the smallest of the atoms presented here
6. I have exactly 3 valence electrons
7.I am the most paramagnetic of the atoms presented here
8. I am the copper
9.I am isoelectronic with Ti2+
IF
cessibility: Good to go
IA
16
SIS
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a. The determination of calcium in milk.
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A 10.00-g sample containing an analyte was
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diluted to volume. When a 10.00-mL aliquot of the
resulting solution was diluted to 25.00 mL it was
found to give a signal of o.235 (arbitrary units). A
second 10.00-mL aliquot was spiked with 10.00 mL
of a 1.00- ppm standard solution of the analyte and
diluted to 25.0o mL. The signal for the spiked
sample was found to be o.502. Calculate the weight
percent of analyte in the original sample.
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during periods of heavy traffic in large cities?
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contribute to the poor air quality experienced in many of India's major cities?
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1. a 50.00-mL sample containging an analyte gives a signal of 11.5(arbitrary units). A second 50-mL aliquot of teh sample, which is spiked with 1.00-mL of a 10.0-ppm standard solution of the analyte, gives a signal or 23.1. what is the concentration of analyte in the original sample?
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Related Questions
- NAME: LAB SECTION: EXPERIMENT 21: Spectroscopy of Dyes POSTLAB EXERCISE 1. AUV/V is a spectrometer used to measure the absorbance of a solution of hemoglobin bound to oxy- gen at 590 nm. The molar absorptivity was determined to be 8.57 × 104 M-'cm-. The cuvette used had a 1.00-cm sample pathlength. The measured absorbance was ), 140. What was the concentra- tion of hemoglobin in the solution? 2. What would be the absorbance of the solution in question 1 if the pathlength were 2.00 cm? nohntooote 196arrow_forwardQuestion 19 of 27 > Graphs are a common data analysis tool used in all branches of science. The given graph represents how light absorbance at 423 nm varies with concentration of an organic dye. Identify the parts of the graph. Answer Bank y = 0.210x – 0.032 line equation Title Abs423 Dye Conc. (nM) best-fit line 0 i 2 3 4 5 6 7 8 9 10arrow_forwardTimer Course Contents » ... » Data, Statistics, and Graphing » Graphing Absorbance (Optical Density) versus Molar Concentration The following table contains some data showing the effect of molar concentration of a solution on the absorbance (optical density) reading from a spectrophotometer. No ↑ Does the solution in your graph follow Beer's Law? Concentration (M) Submit Answer Incorrect. Tries 37/99 Previous Tries 0.13 0.28 0.43 0.58 0.73 0.88 1.03 1.18 1.33 1.48 What is the concentration of a solution that has an Optical Density of 0.21? (Give units in your answer). 0.21M You are correct. Your receipt no. is 155-7520 ? What is the concentration of a solution that has an Optical Density of 0.91? (Give units in your answer). 0.81 M Previous Tries Optical Density To assist in graphing, right click on this excel spreadsheet file, save it to your computer, and open it in Excel. Enter the above data in the appropriate spaces in the spreadsheet. Select the graph that is created, and print…arrow_forward
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