29. Labels. Suppose that you had an in vitro fatty acid- synthesizing system that had all of the enzymes and cofactors required for fatty acid synthesis except for acetyl CoA. To this system, you added acetyl CoA that contained radioactive hydrogen (³H, tritium) and carbon 14 (¹4C) as shown here. 3H O 14 3H-C-C-SCOA 3H The ratio of ³H/¹4C is 3. What would the 3H/14C ratio be after the synthesis of palmitic acid (C₁6) with the use of the radioactive acetyl CoA?

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter23: Fatty Acid Catabolism
Section: Chapter Questions
Problem 4P: Examining the Labeling of Glucose from 14C.labeled Acetate Even though acetate units, such as those...
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29. Labels. Suppose that you had an in vitro fatty acid-
synthesizing system that had all of the enzymes and cofactors
required for fatty acid synthesis except for acetyl CoA. To
this system, you added acetyl CoA that contained radioactive
hydrogen (³H, tritium) and carbon 14 (¹4C) as shown here.
3H O
14
3H-C-C SCOA
3H
The ratio of ³H/¹4C is 3. What would the ³H/14C ratio be
after the synthesis of palmitic acid (C₁6) with the use of the
radioactive acetyl CoA?
Transcribed Image Text:29. Labels. Suppose that you had an in vitro fatty acid- synthesizing system that had all of the enzymes and cofactors required for fatty acid synthesis except for acetyl CoA. To this system, you added acetyl CoA that contained radioactive hydrogen (³H, tritium) and carbon 14 (¹4C) as shown here. 3H O 14 3H-C-C SCOA 3H The ratio of ³H/¹4C is 3. What would the ³H/14C ratio be after the synthesis of palmitic acid (C₁6) with the use of the radioactive acetyl CoA?
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