50 uL of an aqueous sample of double-stranded DNA is dissolved in 950 uL of water. This diluted solution has a maximal absorbance of 0.326 at 260 nm. What is the concentration of the original (more concentrated) DNA sample, expressed in ug/ul? I would like to know the process of this question. And I was wandering as well if the total diluted volume would be 950ul + 50ul = 2000ul
50 uL of an aqueous sample of double-stranded DNA is dissolved in 950 uL of water. This diluted solution has a maximal absorbance of 0.326 at 260 nm. What is the concentration of the original (more concentrated) DNA sample, expressed in ug/ul? I would like to know the process of this question. And I was wandering as well if the total diluted volume would be 950ul + 50ul = 2000ul
Macroscale and Microscale Organic Experiments
7th Edition
ISBN:9781305577190
Author:Kenneth L. Williamson, Katherine M. Masters
Publisher:Kenneth L. Williamson, Katherine M. Masters
Chapter14: Ultraviolet Spectroscopy, Refractive Indices, And Qualitative Instrumental Organic Analysis
Section: Chapter Questions
Problem 4Q
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50 uL of an aqueous sample of double-stranded DNA is dissolved in 950
uL of water. This diluted solution has a maximal absorbance of 0.326 at 260 nm. What is
the concentration of the original (more concentrated) DNA sample, expressed in ug/ul?
I would like to know the process of this question. And I was wandering as well if the total diluted volume would be 950ul + 50ul = 2000ul
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