9. You add 1.5 mL of 200 µM NADPH to 1.5 mL of a solution with an unknown concentration of oxidized glutathione, GSSG, and a catalytic amount of glutathione reductase. The final absorbance of the solution was 0.25 at 340 nm in a 1 cm cuvette. What was the concentration of GSSG in the original 1.5 mL? The reaction catalyzed by glutathione reductase is: GSSG + NADPH + H+ → 2 GSH + NADP+ The relevant extinction coefficients are: NADPH, €340 = 6220 M-¹ cm-¹ NADP+, €340 = 0

9. You add 1.5 mL of 200 µM NADPH to 1.5 mL of a solution with an unknown concentration of oxidized glutathione, GSSG, and a catalytic amount of glutathione reductase. The final absorbance of the solution was 0.25 at 340 nm in a 1 cm cuvette. What was the concentration of GSSG in the original 1.5 mL? The reaction catalyzed by glutathione reductase is: GSSG + NADPH + H+ → 2 GSH + NADP+ The relevant extinction coefficients are: NADPH, €340 = 6220 M-¹ cm-¹ NADP+, €340 = 0

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter9: Atomic Absorption And Atomic Fluorescence Spectrometry

Section: Chapter Questions

Problem 9.6QAP

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