Absorbance A calibration curve for the absorbance of an unknown is shown below. What would be the concentration of a sample with absorbance of 0.45. Give the answer with the correct unit. 1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 Absorbance vs. Concentration 0 1 2 3 4 5 6 7 8 9 Concentration (mM) 10 10 11
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A calibration curve for the absorbance of an unknown is shown below. What would be the concentration of a sample with absorbance of 0.45. Give the answer with the correct unit.
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- I do not understand the results of this experiment, explain what the results of this experiment are and the purpose Table 2 Absorbance vs CoCl2 concentration Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138 7 (Unknown) 0.088explain what the results of this experiment are and the purpose Table 2 Absorbance vs CoCl2 concentration Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138 7 (Unknown) 0.088Compute for the sodium concentration of the patient. Use the same formula in the computation of the concentration using absorbance and concentration of the standard. Given: Standard sample Concentration : 150mmol/L Absorbance: 0.112 Patient's sample Absorbance : 0.226 Concentration : ??
- You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equationCalculate the amount of BSA you will add to the tube to get the concentrations listed on the chart. Your stock tube is 2mg/mL. The amount of water will be the amount to bring your total volume up to 100uL. Standard Concentration BSA H2O Total volume A 200 ug/mL 100uL B 150 ug/mL 100uL C 100 ug/mL 100uL D 50 ug/mL 100uL E 20 ug/mL 100uL F 10 ug/mL 100uL G 5 ug/mL 100uL H 0 ug/mL 0 uL 100uL 100uLSolution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…
- A nurse administered 4.80 mLmL of a radioisotope solution that has an activity of 195 μCi/mLμCi/mL . What total dose of the radioisotope did the patient receive? Express your answer to three significant figures and include the appropriate units.Based on the following data, what is the MRSA concentration (CFU/ml) in the patient's blood sample? Write your answer in the following format: Value x 10^Exp. Do not include units. 100 μl 100 μl 100 μl 2.8X10^2 A Original 100 μl 100 μl Blood Sample 100 μl B 900 μl c 900 μl D 900 μl E 900 μl F 900 μl Saline Tubes Lawn of growth 000 100 μl 200080 100 μl 100 μl | 100 μ. | 100 με 280 22 2 0 colonies colonies colonies colonies LB + MethicillinBSA standard Bradford (uL) Distilled Average Tube water (uL) reagent (mL) Absorbance 1 500 0.00700 2 490 10 5. 0.15720 480 20 5. 0.35035 4 470 30 0.50745 460 40 5 0.71540 450 50 5 0.89290 Sample 300 0.24201 5. 3.
- Construct a standard curve (absorbance versus protein concentration in the standards) Conc. (mg/ml) 0 0.25 0.5 0.75 1.0 Absorbance 0.000 0.126 0.249 0.361 0.464The physician orders Cipro oral suspension 250 mg bid for 3 days for a uti. You have Cipro 5g/100 mL. How many mL will you administer per dose?A nurse wants to know the what volume of Water for Injection, BP to be added to a vial containing 500mg doxorubicin to produce an injection with a concentration of 2.5 mg/mL. The displacement volume of doxorubicin is 0.3 mL/10 mg.