Application Of DNA fingerprinting 1. Look at the diagrams below and indicated if the child came from the father in question? Provide evidence for your reason. A) M C F 400000000000 an wwwwwwww M C www.wer 1/com F 250000000000 395 ASOR wwwwwww
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- I'm stuck on A1. Question what notation would you use to characterize patient a's karyotype? I put 45, xx/xy +13 but I'm not sureCan you please check my answer and make sure it is correct. Question: How can DNA evidence be used to convict or exonerate a defendant? Why is DNA evidence so powerful? Answer: DNA evidence can be used to perform DNA profiling to determine the genotype of the specific DNA sample. With just a small amount of DNA, PCR can produce billions of copies of that specific segment. The segments that are used are from non-coding regions that contain STR’s or short tandem repeats. These very short DNA sequences are repeated and are specific to individuals because we inherit them from our mother and father. Gel electrophoresis separates the PCR products based on their size and each band is compared to the allele ladder. This process helps to identify the alleles present in the original samples. DNA profiling is performed at many loci to be able to tell the genetic difference between different individuals with a lot of certainty. The DNA from the different suspects is compared to the allele…a. DNA fingerprinting is a method of visualizing similarities and differences in the DNA sequence of individuals. Arrange the following items so that they show the DNA fingerprinting steps in proper order: - Gel electrophoresis - Stain or probe for DNA - cut DNA into fragments - Amplify DNA sample using PCR b. On the gel shown below are four DNA samples. Samples A to C are taken from tissues of landslide victims that are being identified, while sample D came from a hair sample brought by a mother looking for the remains of her son. (see img) i. If similar band patterns in a gel are created using the same restriction enzyme, what does that tell you about the DNA sequence of the samples? ii. In sample C, only two fragments were created. How many restriction sites (regions where enzymes cut) are present in sample C?
- Examine the gel from a Rape investigation below. If you were the DNA analyst you would conclude that: DNA size markers blood sexual assault evidence samples victim |suspect A suspect B female fracțion male fraction 1 2 3 4 5 6 A. Suspect B is excluded as the source of the evidence, but Suspect A cannot be excluded. B. Both Suspects A and B are excluded as the source of the evidence. C. Suspect A is excluded as the source of the evidence, but Suspect B cannot be excluded. D. Suspect B cannot be excluded as a source of the evidence. The results with Suspect A are inconclusive. E. Neither Suspect A or B can be excluded as a source of the evidence.Answer the following questions. 1. Explain the steps associated with DNA profiling and state its advantages and disadvantages, 2. Describe how mutations are linked to DNA polymorphism. GUIDELINES: Each answer should be 250 words in length. Content should not be copied. References and bibliography should be provided for the content and images. please asapExplain whether the woman having genes for high risk for developing cancer and Alzheimer disease is morally or legally obligated to tell her children and close relatives about the genetic testing.
- 3 app.formative.com/formatives/63763915dfee3ba940357e25 4 5 6 7 8 9 10 11 Type here to search Sample 1 | | |||||||||||||||| || || | ||||||| ||| |||||||| Sample 2 19. An individual found with blood on her clothing was accused of a crime. DNA fingerprinting of the following samples was carried out: Sample 3 Sample 1: Blood of the accused individual Sample 2: Blood of the victim Sample 3: Blood sample found at the crime scene Sample 4: DNA evidence found on the accused individual's clothing Sample 5: DNA evidence found in the accused individual's car |||||||||||||||||| | || | Sample 4 12 13 14 Sample 5 O 15 16 17 18 19 20 21 * Is there DNA evidence to support the arrest of the accused individual for this crime? Q B faAnswer each of the following correctly. Designer Genes Work (This is all about Applications of Recombinant DNA) 1. How does DNA Replicate? 2. What is Genetically Modified Organism (GMO)? 3. Illustrate your own Designer genes based on the following: 1. Identify a special trait. 2. Identify a source organism. 3. Identify a target orgsnism 4. Identify the modified/added trait.Answer each of the following correctly. Designer Genes Work (This is all about Applications of Recombinant DNA) 1. How does DNA Replicate? 2. What is Genetically Modified Organism (GMO)? 3. Illustrate your own Designer genes based on the following: 1. Identify a special trait. 2. Identify a source organism. 3. Identify a target orgsnism 4. Identify the modified/added trait. Example: Hot Tomato > Chili > Tomato > Spicy Tomato (Look to the picture I provided for this)
- The key portion of the autoradiograph from a single locus probe analysis of various DNA samples in a rape investigation is shown in this figure. (Please refer to the attached Picture) Samples of DNA were loaded into the following lanes: (based on the picture) known blood sample of victim known blood sample from defendant DNA size markers female fraction from vaginal swab of victim male fraction from vaginal swab of If you are the DNA analyst, you should conclude that: A. The suspect is guilty B. The suspect might be guilty, but more probes should be used. Explain your answer and Provide Conclusion.State the five basic steps of DNA fingerprinting using the RFLP method. Why do you think the PCR method is of more use in crime scene investigations?questions below: Aura and Ryan are expecting a child. Aura has dark eyes and Ryan has light eyes. Aura's mother has light eyes. Draw a Punnett square and be sure to include the following information for full credit: 1. Place the parents alleles at the top and on the left side. 2. Write in the allele crosses inside the Punnett squares. 3. Write out the genotypes probabilities for an offspring. 4. Write out the phenotype probabilities for an offspring. 5. What is the probability that Aura and Ryan could have a child with light eyes, even if light is recessive to dark? Note: For this question, you will have to go further back into the family tree to get Aura's genotype.