Calculate the approximate MW of a protein with 341 amino acid residues and express it ing/mol and in kD. Show complete solution.
Q: Applying: Which of the following commonly acts as the precursor for the synthesis of vitamin C (asco...
A: Carbohydrates are compounds formed of monosaccharides as building blocks, which range from simple su...
Q: Find a protein of your choice (rcsb.org), choose a part of it (containing at least 30 amino acid res...
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids conta...
Q: (Vmax.f [S] - (Vmax,b [P] KM,b [P] vo [S] 1+ KM.f KM.b ||
A: In this enzyme kinetics, I am going assume a general mechanism and then derive the rate equation. As...
Q: In SOAP Experiment, Do you think the type of fat/oil used will make a difference in the product? Why...
A: Soaps are sodium salts of fatty acids and are form by the saponification reaction.
Q: Which of the following pairs are correct? Ribulose:Aldotriose; Tagatose:Aldopentose Ribose:Ketohexos...
A: Monosaccharides are the simplest carbohydrate units. They contain elements such as carbon, hydrogen ...
Q: Which of the following is the odd one out? NADP FMN NAD
A: Introduction: A coenzyme is a cofactor that consists of small organic molecules, many of which are d...
Q: 9. Which of the following molecules cannot be found in cell membrane? A. Triglyceride B. Cholesterol...
A: The cell membrane, commonly known as the plasma membrane, protects the cell. It is found in all cell...
Q: Define the importance of 54-nucleotide element ?
A: Most living organisms and viruses include the molecule ribonucleic acid (RNA). Nucleotides are the b...
Q: Catalase is an enzyme that catalyzes hydrogen peroxide to become oxygen and water. Which type of spe...
A: Enzymes are biological catalysts that increases the rate of biochemical reaction without itself bei...
Q: Describe the skeletal system and the role it plays during cell division.
A: The cellular cytoskeleton is constituted of microtubules, microfilaments and intermediate filaments....
Q: how would you identify whether or not complete resolution of the three amino acids has been achieved
A: An amino group and an acid group-containing organic molecules are called Amino acids. when ...
Q: What is vaccine? Please be sure to include what molecules are present and the biological mechanism b...
A: Immunity is described as a complicated biological system that has the ability to recognise and accep...
Q: 5. You are imprisoned in a biochemistry lab by an ogre (one of Shrek's kids) who wants you to purify...
A: Proteins are polymers of amino acids linked by amide or peptide bonds. pI of the proteins are the pH...
Q: Give examples of drugs that are monoamine oxidase (MAO) inhibitors
A: Monoamine oxidases are certain class of enzymes that are involved in the removal of neurotransmitter...
Q: 8. "Salting-out" is a process A. to represent the certain molecules are ionized in a solution B. whe...
A: Salt is a molecule composed of positive and negative ions when dissolved in water. Salt molecules ar...
Q: Glycocongugates are, by definition: Question 26 options: Carbohydrates covalently linked to bi...
A: Carbohydrates are macronutrients that are used to produce energy in metabolic activities. Monosaccha...
Q: Compare and contrast direct fecal smears (DFS) and D' Antoni's Method for stool examination
A: A direct fecal smear is used as a quick screening test to check for any intestinal parasite. Protozo...
Q: ive examples of drugs that are monoamine oxidase inhibitors.
A: Enzymes are highly specific. All cellular reactions or processes are mediated by en...
Q: Understanding: Non-polar molecules are insoluble in water. This is because:
A: "Since you have asked multiple question, we will solve the first question for you. If you want any s...
Q: Which of the following differentiates amylose with cellulose? The two polysaccharide have different ...
A: Amylose : It is a storage polysaccharide Cellulose : It is a structural polysaccharide
Q: 2. A biochemical analysis of an RNA sample showed 40 % ofnitrogenous bases were cytosine (C). What p...
A: Nucleic acids are macromolecules. These are of two types - Deoxyribonucleic acid (DNA) and Ribonucle...
Q: Answer Questions about your results: 1. Which pH provided the highest pepsin activity? Is that what ...
A:
Q: The enzyme that removes the RNA primer from the Okazaki fragment is: DNA pol III DNA ligase ...
A: Introduction: DNA is the hereditary material that defines every cell. It is found within the nucleus...
Q: 1. The graph given represents a size-exclusion chromatogram after the refolding of the hemoglobin te...
A: Chromatography is a technique of protein purification in which protein can be separated based on its...
Q: .Linoleic acid and linoleic acid cannot be synthesized in human tissues, because of _____. * a.Hum...
A:
Q: Streptococcus pneumoniae cells of genotype str s mtl - are transformed by donor DNA of genotype strr...
A: It is the genetic alteration of a cell resulting from the uptake and expression of foreign genetic m...
Q: H. Draw a plot showing reaction velocity as a function of substrate concentration for Ks = 50 µM, Vm...
A: First we need to find the values of (V0) at various substrate concentrations ([S0]). For this we dra...
Q: Brick-red colour appeared in Benedict's test indicates that glucose is oxidized inthe solution. 4.
A: “Since you have asked multiple question, we will solve the first question for you. If youwant any sp...
Q: How do the standard amino acids differ from one another? O in whether or not the amino group is chir...
A: Peptides and proteins are made up of amino acids, which are molecules or building components. An ami...
Q: 1. Cite five diseases that could be diagnosed with the determination of enzyme concentration. 2. Giv...
A: Note : Hi ! Thank you for the question. We are authorized to answer one question at a time. Since yo...
Q: adjust blood pH on the order of hours to days. Secretion of outside the kidneys via ion exchanger to...
A: For appropriate physiological functioning, the concentrations of acids and bases in the blood must b...
Q: What is the effect of the NDM gene?
A: NDM (New Delhi Metallo-β-lactamase) gene, found in Enterobacteriaceae, is a genetic element encodin...
Q: Question:- 1. Transcribe and translate the given region of DNA, note that the promoter region is to...
A: The Central dogma of molecular biology involves the synthesis of RNA using DNA as template by the pr...
Q: n comparison to glycogen, fats can provide more energy for the body per carbon atom. Why? 1. Fat...
A: Fats belong to a group known as lipids. It is characterized by a greasy feel and they are insoluble ...
Q: 1. Based on your knowledge about gums and mucilages, how will you differentiate resins from them? (O...
A: Q. 1. Mucilages are generally normal products of metabolism (physiological products), formed within ...
Q: Why is replication called semi-conservative? each new molecule of DNA contains one template stran...
A: Introduction - To transfer the genetic information from a parent cell to a daughter cell during rep...
Q: 1. In a protein, why does when Ala is replaced with Ile, it loses its activity but when Lys is repla...
A: Note : Hi ! Thank you for the question. We are authorized to answer one question at a time. Since yo...
Q: Based on the given results, what do you think is the isoelectric pH of casein? Briefly discuss your ...
A: Casein is a key protein of milk and present in large amounts in cheese. Proteins are polymers of ami...
Q: what does the term excitation- coupling mean?
A: The term was first coined in the year of 1952 by Alexander Sandow, he was a researcher in the field ...
Q: Phytochemical test Physical description of extract: Color: Reddish brown Odor: Aromatic Transparency...
A: Phytochemical tests are the confirmatory tests for the presence of different components like alkaloi...
Q: 1.Fructose and galactose can be distinguished by which of the following reagents? * a.Fehling’s reag...
A: Both glucose and fructose are monosaccharides. Glucose is aldohexose and fructose is a keto hexose. ...
Q: Rhodium and other transition metals are generally used in heterogeneous/homogeneouslenzyme catalysis...
A: Enzymes are the biocatalyst which promotes the biochemical reactions in any living organism. The enz...
Q: True or false? Initial velocity studies are particularly useful for investigating the kinetics of an...
A: Enzymes are protein catalysts. Enzyme kinetics explores how experimental settings affect response ra...
Q: 6. Amino acid alanine containing NE isotope in a-amino group was administrated to mouse with food. I...
A: The use of radioactive labelling of particular atoms have often been used to determine metabolic pat...
Q: TRUE OR FALSE Coconut oil will give (positive) result to Molisch test.
A: Molisch test is used to detect presence of carbohydrate in a mixture or sample. This test is based o...
Q: Remembering: CO2 in our body is transported through the blood primarily as O co2 myoglobin complex O...
A: Introduction: The waste product of aerobic respiration is carbon dioxide. Very high or low amounts o...
Q: Answer Questions about your results: 1. Which pH provided the highest pepsin activity? Is that what ...
A: Pepsin is an endopeptidase enzyme that converts proteins into peptides. It is formed in the main cel...
Q: 1a-Consider a situation where the primer sequences used in a PCR reaction are synthesized to be comp...
A: A heritable genetic change in the genetic material of an organism that gives rise to alternate for...
Q: Please explain in detail a chemical reaction between rRNA and another biomolecule
A: rRNA are the structural components of ribosomes. Hence rRNA does not generally participate in chemic...
Q: 8. In patients after prolonged hepatitis, the ALT and AST activities were measured in the blood seru...
A: Hi. Thank you for the question. As per the honor code, we are allowed to answer three sub-parts at a...
1. Calculate the approximate MW of a protein with 341 amino acid residues and express it ing/mol and in kD. Show complete solution.
Step by step
Solved in 3 steps
- 3 i. Define Biological Value (BV) of Proteinsii. What are the advantages of this protein evaluation method when compared to Nbalance and chemical score?5iii. A bull consumes 7 kg DM of feed containing 60 g N/kg. The bull excretes 120 g N inthe faeces and 50 g N in the urine. The feacal and urinary N had 20 g metabolic feacalnitrogen (MFN) and 6 g endogenous urinary nitrogen (EUN) respectively.Calculate the BV of the protein in the diet.. A protein gives, under conditions of buffer composition, pH, and tem- perature that are close to physiological conditions, a molecular weight by sedimentation equilibrium measurements of 140,000 g/mol. When the same protein is studied by SDS gel electrophoresis in the absence or presence of the reducing agent B-mercaptoethanol (BME), the pat- terns seen, respectively, in lanes A and B are observed. Lane C contains standards of molecular weight indicated. From these data, describe the native protein, in terms of the kinds of subunits present, the stoi- chiometry of subunits, and the kinds of bonding (covalent, noncova- lent) existing between subunits. / A B - BME + BME STD Serum albumin, 67,000 Ovalbumin, 43,000 Carbonic anhydrase, 30,000 Trypsin inhibitor, 20,000 10 2. 4. Co cm migratedUsing the data in Table calculate the average amino acid residue weight in a protein of typical composition. This is a useful number to know for approximate calculations
- 16. A quantitative study of the interaction of a protein with its ligand yielded the following results: Ligand concentration 0.5 1 2 3 4 5 6 9. 12 (μM) v (moles of 0.14 0.31 0.48 0.61 0.68 0.72 0.77 0.79 0.81 ligand bound per mole of protein) Plot a graph of ligand concentration versus v. Determine KD, the dissociation constant for the interaction between the protein and its ligand, from the graph.A protein gives, under conditions of buffer composition, pH, and temperaturethat are close to physiological conditions, a molecular weight by size exclusion measurements of 140,000 g/mol. When the same protein is studiedby SDS gel electrophoresis in the absence or presence of the reducing agent β-mercaptoethanol (BME), the patterns seen, respectively, in lanes A and B are observed. Lane C contains standards of molecular weight indicated. From these data, describe the native protein, in terms of the kinds of subunits present, the stoichiometry of subunits, and the kinds of bonding(covalent, noncovalent) existing between subunits.A series of standard proteins and an unknown enzyme were studied by gel filtration on a Sephadex G200 (the 200 refers to the maximum pore size in kDa) column. The elution volume Vel for each protein is given in Table 1 below. (a) Plot the data in the form of log Mr versus elution volume. From the line of best fit through the points for the standards, determine the Mr of the unknown enzyme. Explain why ferritin and ovomucoid behave anomalously. Table 1 - The Vel versus Mr data Protein Mr Vel (mL) Blue dextran* Lysozyme Chymotrypsin Ovalbumin Serum albumin Aldolase Urease Ferritin# Ovomucoid# Unknown 1,000,000 14,000 25,000 45,000 68,500 150,000 500,000 700,000 28,000 ⎯ 85 200 190 170 150 125 90 92 160 139 *Blue dextran is not a protein but a high-Mr carbohydrate that has a covalently bound blue dye, and it elutes with the void volume of the column. # Do not…
- A tetrapeptide, glutamate-glycine-alanine-lysine, is prepared at at concentration of 1 mM (0.001 M) and is measured in the standard setup (pathlength of 1 cm). What is the approximate absorbance of this peptide at 280 nm? Hint: if the peptide contained a single tryptophan, the answer would be about 10. 10 280 1 01. Lactoferrin is an iron-binding glycoprotein that forms the antibiotic fragment lactoferricin during digestion. The protein was isolated from 1.5 liters of ewe skim milk by successive ammonium sulfate precipitation until a final salt concentration of 70% was obtained. Determine the amount (in g) of powdered ammonium sulfate needed in bringing the solution from (a) 0% to 40% saturation and (b) 40% to 70% saturation assuming that the volume remains constant. Initial concentration of (NH4)2SO4, % saturation 0 10 20 30 40 50 60 70 80 90 10 55 Final concentration of (NH4)2SO4, % saturation 30 40 50 60 70 80 Grams of solid (NH4)2SO4 to be added to 1 L of solution 176 242 314 390 472 561 657 117 405 491 584 59 421 351 438 20 113 57 182 121 61 251 325 63 188 126 195 130 65 260 337 270 202 281 135 67 210 140 70 90 761 685 511 609 533 365 292 219 146 HUGH LATLE 100 73 457 381 305 228 152 76A sample mixture consists of three proteins with the following properties: Protein A MW (kDa) 200 Amino acid composition 40% nonpolar, 60% polar B 45 20% nonpolar, 80% polar C 98 85% nonpolar, 15% polar IpH 9 3 5 1. If the mixture is subjected to ammonium sulfate precipitation, which protein will precipitate out first? [Select] 2. If the mixture is subjected to isoelectric focusing, which protein will stop moving nearest to the positive electrode? [Select] 3. If the mixture is subjected to cation-exchange chromatography using a buffer at pH 7, which protein will bind to the resin? [Select] 4. If the mixture is subjected to SDS-PAGE, which protein will be at the bottommost portion of the gel? [Select] * Previous Nex
- 4. a. Give the full names of the amino acids in this peptide. НзN — CH—С-N—CH— С-N—CH—С—О ČH3 H ČH,OH H. CH2 -ОН b. Calculate the isoelectric point (pl) of a peptide with sequence SNARE. Show your work in a table, giving the pKa values of the ionizable groups, pH, and charges. Step marks are counted. Don't need to write “protonated" or “deprotonated".Which of the peptide sequences below best matches the hydropathy plot shown? L 10 Residue Number 4 2 Hydropathy value -2 0 セー RAFLILFMTYFLILFLI ILYYAGSREDHSGYLIL OLHGDQNRERDGHSQERD EQSDTERNQHGALIYLI 01 5 15At which pH would the peptide with the amino acid sequence 'KEYHR' be found in an IPG (Immobilized pH gradient) strip after successful isoelectric focusing. Show calculation. lonizable groups Alpha amine group of K Side chain group of K Side chain group of E Side chain group of Y Side chain group of H Side chain group of R Alpha carboxylic acid group of R pka 8.95 10.53 4.25 10.07 6 12.48 2.17