Differentiate the advantages and disadvantages of messenger ribonucleic acid (mRNA) by RT-PCR and protein via ELISA in cytokine analysis
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Differentiate the advantages and disadvantages of messenger ribonucleic acid (mRNA) by RT-PCR and protein via ELISA in cytokine analysis
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- describe the blue-white colony selection method in the recombinant colony screening, and present a brief argument for its use in biotechnology labs.Explain: Describe an electrochemical sensor assay method for rapid bacterial detection and identification. What are the principles and mechanisms involved? functionalization of a sensor array with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase linked DNA oligonucleotide detector probe. Explain how bacteria are being detected using an electrochemical sensorPlease describe the ChlP assay. Why it is used? Please give examples Cells Lysis Cross-linking Antibody binding Immunoprecipitation Crosslink reversal Wash steps DNA and protein analysis DNA purification and quantitative PCR
- With detail, compare and contrast the following 5 real-time assays; Taqman, SYBR Green, Molecular Beacon, FRET, and Scorpion. Please write complete sentences in paragraph form. Identify how the assays are similar but more importantly, what are the distinguishing features of each of the technologies.**Application of Abs in diagnosis and research: What is to be measured - Ag or Ab? Is the assay appropriate? Is the assay sensitive enough? Reporter strategies? Primary Ab vs 2nd Ab?Nicotinamide Adenine Dinucleotide (NAD) Assay (SIGMA Kit MAK037) analysis of tissue samples initially requires: centrifugation at 13,000g for 10min to homogenise tissue. centrifugation of 20mg of tissue at 2,000rpm with Extraction Buffer. freezing and thawing for 2 cycles of 20min before addition of Extraction Buffer. homogenization of PBS washed tissue with Extraction Buffer.
- What is an advantage of using an ELISA instead of a protein microarray to study a proteome? What is a disadvantage?list the advantages and disadvantafes of latex agglutinaton(LA) and gelatin particle agglutination tests (GPAT) techniquesCompare and contrast the principle behind DNA migration in agarose gel electrophoresis from that of protein migration in SDS-PAGE.