For an enzymatic assay, tumour cells are incubated at different concentrations in individual vials (Table 1). Please note that the final reaction volume differs between the three setups. Vial No. A (ml) В (ml) C (ml) H20 1 Tris/HCl buffer pH7 1 1 1 Tumour cells (5% (w/v)) in buffer 1 3 4 Glucose [25mM] 0.5 0.75 1 Table 1. a) Calculate the weight (in gram/ml) of tumour cells in each vial. Show your workings. b) Calculate the glucose concentration in each vial (in mol/ml). Show your workings.
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- 4. To perform a certain activity assay it is necessary to have 0.25 mL of an enzyme at a concentration of 0.2 mgml¹ in the presence of 0.4 mM substrate and 200 mM NaCl in a suitable buffer. The stock solutions of enzyme, substrate and NaCl are 4 mgmL-¹, 40 mM and 1 M all in the same type of buffer. What volumes of the stock solutions and buffer are required for each assay?1:02 4 Briefly describe how you would make 5op mls of a 1X electrophoresis running buffer from a 15X stock solution.h) i) j) Ph + НО OSI(CH3)3 Br PPH3 1) "BuLi /THF/rt / 1.5 h H CH3 homo /rt / 1 h 2) -Si(CH3)3 OCH3 TiCl4 CH₂Cl₂/-78°C / 15 min PPh3/1₂ (1 eq) imidazole / THE 0°C-it
- 1.Which of the following correlations about the analyses of iodine and saponificationnumbers is/are incorrect?a. Alcoholic KOH: dissolve KOH to lipid sampleb. Hanus reagent: react with saturated lipid bondc. Reflux set-up: hasten reactiond. Blank titration: determine total mol of KOH2. A triacylglycerol (Sample X) was subjected to saponification analysis. 1.1 grams of thesample was refluxed with 5% alcoholic KOH and the refluxed solution was titrated with 17.2 mLof 0.44 M HCl titrant. The blank solution was titrated with 25.6 mL of the titrant.i. What is the saponification number of the sample? ii. Which of the following could be the identity of the sample? Show solutions pls a. Tripalmitin (MW: 807.3)b. Trilaurin (MW: 639.8)c. Triolein (MW: 885:4)d. Tristearin (MW: 891.48)The O2-consumption curve of a dilute, well-buff ered suspension of mito chondria containing an excess of ADP and Pi takes the following form: Sketch the curves obtained when (a) amytal is added at time t = 1 and (b) amytal is added at t = 1 and succinate is added at t = 2.A protein of molecular weight = 55,000 g/mol and at 1 mg/mL concentration was titrated with Ellman’s reagent. What is the molar concentration of the protein in solution?
- Explain the reactions involved in separation separation of benzoic acid from mixture contain 3 components 1.Benzoic acid (acidic nature pKa=4.17) 2.Naphthalene (neutral compound) 3.paraNitroaniline(basic compound pKa=12.99. 100 ml boiled cooled and filtered water sample takes 9.6 ml of M/50 EDTA in titration. The Permanent hardness of the water sample in terms of ppm of CaCO3 equivalent is16) Which of the following(s) is/are correct? I- Selectivity factor is the amount of time a solute spends in the stationary phase relative to the time it spends in mobile phase II- The retention time for an analyte is the time interval between its injection onto a column and its appearance at the detector at the other end of the column. III- Distribution constant is the ratio of the total solute concentration in the stationary phase to that in the mobile phase. A) I, II and III B) Only II C) I and III D) II and III E) Only III for GC2
- A phlebotomist was acting as an instructor for a phlebotomy student from the local college and was anxious to help her learn all the special tests that had not been practiced in class. When a stat blood culture and electrolytes were ordered on a patient in the ICU, the instructor quickly grabbed the student and they headed for the floor. It was clear after looking at the patient that they would have to use a butterfly in a hand vein to collect the test specimen. The instructor was busy helping the student prepare the site and did not pay attention to the order the student layout all of the equipment/tubes/bottles for the draw. The student elated that she was able to access the difficult vein and that blood was flowing freely to the anaerobic bottles. After filling both it and the aerobic bottle to the proper level, she proceeded to draw a sst tube for the electrolytes. The blood flow was now very slow, with blood entering the tube one drop at a time. After patiently waiting for the…The Kjedahl procedure was used to analyze 256 µL of a solution containing 37.9 mg protein/mL. The liberated NH3 was collected in 5.00 mL of 0.033 6 M HCl, and the remaining acid required 6.34 mL of 0.010 M NaOH for complete titration. What is the weight percent of nitrogen in the protein? wt%(14) The Ksp of PbCl2 is 1.6 x10-5 at 25°C. What is the concentration of Pb2+ (in M) in an aqueous solution initially containing 0.13 M Na+ and 0.13 M Cl- and now saturated with PbCl2?