Give at least two common loading control used in Western Blotting?
Q: 1. Which of the pairs of amino acids gives a positive result to Xanthoproteic test?* Tyrosine and…
A: Biological Macromolecules are constituted of nucleic acids, proteins, lipids and carbohydrates.
Q: Determine the cause-effect relationship of the following variables given. Choose the best answer…
A: Gout is a Metabolic disorder that is associated with increased serum concentration of uric acid that…
Q: Carbonic anhydrase has an acidic zinc canter compared to carboxypeptidase. Mention the statement is…
A: Carboxypeptidase A is a hydrolase that hydrolyzes the peptide bond at a polypeptide or protein's…
Q: Differentiate between collagen, a-keratin and B-keratin and further tabulate in differences using a…
A: Alpha Keratin, Beta keratin and Collagen are fibrous proteins. Their polypeptide chains are arranged…
Q: Which of the following is the tri peptide GUG- CGA- CCC codons synthesized and decoded in the…
A: A codon table can be used to translate a genetic code into a sequence of amino acids. The standard…
Q: TRUE OR FALSE = "The initiation codon for both prokaryotes and eukaryotes is AUG coding for the same…
A: The triplet codon AUG always codes for methionine which is also called the start codon for…
Q: Hoechst 33342 is a membrane-permeant dye thatfluoresces when it binds to DNA. When a population…
A: The most well-known and most used dye for the visualization of DNA is ethidium bromide. It can…
Q: Macromolecule Proteins Amino +H H H Nucleic Acids phe Carboxyl 0 Monomer(s)/ Subunit(s) and Name of…
A: There are 4 types of Biomacromolecules. They are; Proteins Nucleic Acids Carbohydrates and Lipids…
Q: Carbohydrates are biomolecules are complex molecules whose building block is the ___________.…
A: Carbohydrates, lipids, proteins, and nucleic acids are the four major biomolecules. All of these…
Q: Explain what is meant by "The Central Dogma"? In your explanation, describe the structure and…
A: DNA acts as the carrier of genetic information from one generation to the other. Hence it is…
Q: How is ATP produced in Glycolysis? Kreb Cycle? Electron transport system?
A: Glycolysis is the metabolic pathway in which glucose is oxidized into pyruvate. The citric acid…
Q: Apart from p53, mention and describe the function of three tumor suppressor genes you know
A: The cancer related genes can be divided into two broad categories like the proto-oncogenes and tumor…
Q: Based on intermolecular attractions, why is honey, which is made up of carbohydrates, 2,000 times…
A: A fluid's viscosity is a measurement of its resistance to deformation at a certain rate. As a…
Q: 2) When a certain freon used in refrigeration was expanded adiabatically from an initial pressure of…
A: Joule Thomson coefficient is also called as Kelvin-Joule effect. It is the change in temperature…
Q: Please don't copy Chemistry All of the following tend to favor a helical conformation of a single…
A: Introduction: A longer nucleic acid is called a polynucleotide. It is a biopolymer composed of…
Q: BIOMOLECULES - Please answer the questions properly. - Multiple choice 1. One of the following…
A: Introduction: All proteins are made up of basic secondary structure units, which can be either…
Q: A cell sample suspended in a buffer solution was centrifuge at 12,000 rpm. Which of the following…
A: Centrifugation is a frequently used techniques in the cell and molecular biology laboratory. It is a…
Q: ein isolation an Technique Isolation techniques Centrifugation Salting out Isoelectric precipitation…
A: The purification of a protein is important for determining its structure and characterization of its…
Q: 22. The blood group by the MN system depends on the combi- nation of the codominant genes CM and CN.…
A: For the blood grouping by MN system, According to hardy Weinberg equation, a gene that exists as two…
Q: 4. Use the cartoon protein below to answer the following questions. Assume the rounder subunits are…
A: Proteins are polymers of amino acids linked by peptide/amide bond (Covalent). Primary Structure of…
Q: Which of the following is the tri peptide GUG- CGA- CCC decoded in the ribosome?…
A: A codon table can be used to translate a genetic code into a sequence of amino acids. The standard…
Q: Identify and briefly describe three different PEGylation strategies you can use to PEGylate this…
A: The process of PEGylation improves drug solubility and decreases immunogenicity of a molecule that…
Q: why steroids do not show luminescence on thin layer chromatographic plates?
A: Infrared absorption spectroscopy also stated as IR spectroscopy is one of the most valuable…
Q: Statement 1 - Trans fat may actually be worse for heart health than saturated fats Statement 2- FDA…
A: The purposes of metabolism is the conversion of the energy in food to run cellular processes, the…
Q: Why is DNA so important
A: DNA is an abbreviation for deoxyribonucleic acid. It includes nucleotides, which are biological…
Q: 1. Which of the following statements is TRUE in the catabolism of cytidine triphosphate (CTP)? A.…
A: There are two MCQs so we will solve both the MCQs.
Q: Explain the four stages of external respiration and identify the gradients (driving force) and…
A: The process of breathing is known as respiration. It entails taking in oxygen and exhaling carbon…
Q: Loss of muscle tone is associated with which deficiency? O All deficiencies mentioned O None of the…
A: Loss of muscle tone means decreased muscle activity or less muscle activity. Loss of muscle tone is…
Q: These family of viruses contain DNA as their genetic material, EXCEPT: O All of these contain DNA as…
A: A virus is an infectious microorganism made up of a nucleic acid segment covered by a protein coat.…
Q: Enzyme elastase cleaves polypeptides after ______________ aa. small Positively charged…
A: Enzymes such as elastases have a tendency to leave after aliphatic amino acids. These amino acids…
Q: Which of the following amino acids undergoes phosphorylation? Histidine Glutamic Acid…
A: Post-translational modification of proteins refers to the covalent enzymatic modification of…
Q: Determine the cause-effect relationship of the following variables given. Choose the best answer…
A: Denovo biosynthesis of purines proceed through synthesis of IMP, Inosine mono phosphate. IMP is…
Q: What is the net electric charge of a peptide that has the sequence and provide the one letter code…
A: The amino acids are composed of an amino group, a carboxyl group, a hydrogen atom, and a variable…
Q: Peptide (amide) bonds are foundational to biochemistry. Use the instructions for drawing peptides to…
A: chain of amino acids are linked by peptide bonds and physiological pH, the C-termini and N-termini…
Q: electron transport chain,
A: The ETC stand for electron transport chain system. It helps to transfer the electron from various…
Q: Name all the present Functional Groups in Monosodium Glutamate (MSG) and describe how this…
A: The sodium salt of glutamic acid is monosodium glutamate. This glutamic acid form can be found…
Q: epinephrine/ glucagon
A: The bioactive molecules which helps to catalyze the product in faster rate is called enzyme. The…
Q: Draw 3 isomers of C7H12O2 then state where you wil disposal should only be repeated once).
A: Heptanedial is represented by C7H12O2. The molar mass of the given compound is found to 128.2 g/mol.…
Q: [ PHYSICAL EDUCATION ] 1. Why is it important to train our anaerobic lactic energy system? Cite at…
A: The lactic acid system is defined as an anaerobic energy system. Here, there is a high-energy…
Q: Lipogenolysis is the breakdown of lipids to acetyl CoA true or false
A: Triacylglycerol is an important storage form of energy in the body.
Q: The Km of liver glucokinase is _____ than typical blood glucose concentrations. At typical blood…
A: Glucokinase, gluco is glucose and kinase is an enzyme which is known to phosphorylate a substrate.…
Q: raw a schematic diagram on glycogenolysis by showing the structural formula of its substrate. Show…
A: Glycogenolysis is the process in which stored glycogen is broken down into glucose.
Q: The mannose 6-phosphate (M6P) receptors are crucial for delivering lysosomal proteins to lysosomes,…
A: KDEL is a target peptide sequence in mammals and plants located on the C-terminal end of the amino…
Q: escribe the catalytic reaction mechanism of the enzyme acetylcholinesterase. Discuss the functional…
A: Acetylcholinesterase(AChE) is an enzyme found in the synapses between nerve cells and muscle cells.…
Q: BIOMOLECULES - MULTIPLE CHOICE - Please answer properly QUESTION : Which of the following processes…
A: De novo pathway and salvage pathways are two pathways used for nucleotide biosynthesis. In…
Q: QUESTION : Which of the following best describes the transition state of a catalyzed reaction? A.…
A: Enzymes are mostly proteins that hasten the chemical reactions in our bodies.
Q: Show the complete reaction mechanism (including arrow pushing) for this reaction.
A: Deamination is the process of removal of the amino group from the amino acids as NH3. The liberated…
Q: Which of the following form Right-handed alpha helices? ds DNA, ds RNA, and proteins…
A: Right handed alpha helixes consist of the different amino acids in the proteins or the different…
Q: The bond formed between glycerol and fatty acid chain is a/an _____ bond. Ether…
A: Glycerol is an organic molecule that consists of three hydroxyl groups while the fatty acids contain…
Q: As soon as lysis occurs, proteolysis, dephosphorylation and denaturation begin. These events can be…
A: Cell lysis is a process in which cells membrane are ruptured and all constituents are released. It…
Give at least two common loading control used in Western Blotting?
Step by step
Solved in 2 steps
- For the hybridization step in the Southern blotting, the hybridization was done at 85 degrees Celsius instead of 58 degree Celsius. Explain how this would affect my results.1) Enhanced GFP protein has: An excitation peak at 488nm and emits maximally at 507nm. An excitation peak at 488nm and emits maximally at 587nm An excitation peak at 488nm and emits maximally at 567nm. An excitation peak at 458nm and emits maximally at 507nm. 2) A typical sequencing read will yield _____ nucleotides of unambiguous sequence. 1800-2000 3800-4000 2800-3000 800-1000 3) Sequencing primers should be designed to bind where? ~ 25 nucleotides upstream of the beginning region to be sequenced. ~ 25 nucleotides downstream of the beginning region to be sequenced ~ 60 nucleotides upstream of the beginning region to be sequenced. ~ 60 nucleotides downstream of the beginning region to be sequencedFor the CPT code assignment 44970, which of the following coding references can be accessed from the coding summary screen? Select all that apply.
- What do you mean by Southern Blotting technique?Why are there multiple results returned when you input a sequence into BLAT or BLAST? How do you know which is most relevant? Does that make the others irrelevant?Discuss which barcodes to use for bacteria, animals, plants and fungi and why with references (1000 words)