PO mod 5' 787 18 32 806 789 bases P3 mod P5 3' 1542 1507 1487 721 bases FIG. 1. Diagram of locations of primers on Escherichia coli 16S rDNA, an open reading frame that is 1,542 bases long. The locations of the modified priming sites used for the PCR are shown. Pomod primes at site PO with an extension going to the right. P3mod primes at site P3 with the reaction going to the right; PC3mod primes at the same site with the reaction going to the left. PC5 primes at site P5 with the reaction going to the left. The designations 5' and 3' and base numbering refer to the sense strand.
PO mod 5' 787 18 32 806 789 bases P3 mod P5 3' 1542 1507 1487 721 bases FIG. 1. Diagram of locations of primers on Escherichia coli 16S rDNA, an open reading frame that is 1,542 bases long. The locations of the modified priming sites used for the PCR are shown. Pomod primes at site PO with an extension going to the right. P3mod primes at site P3 with the reaction going to the right; PC3mod primes at the same site with the reaction going to the left. PC5 primes at site P5 with the reaction going to the left. The designations 5' and 3' and base numbering refer to the sense strand.
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications
Section18.1: Key Dna Technologies For Making Genetically Altered Organisms
Problem 2SB
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Question
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Figure 1 from Wilson et al. (1990) shows the locations of the primers and the length of the PCR fragments for Escherichia coli. Redraw Figure 1 to show the primer locations and the PCR fragments lengths as it would be for Coxiella burnettii. This image is from the
PCR Article - Wilson et al (1990) article
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