Production of a recombinant protein by E. coli is proportional to cell growth. Ammonia is used as a source of N and glucose as a source of C, under aerobic conditions. The recombinant protein has a general formula CH1.55N0.31O0.25 and the cell CH1.77N0.4900.24. The yield of biomass from glucose is 0.48 gcel/gglic, and the yield of recombinant protein from glucose is about 20% of that for biomass. a) How much ammonia is needed to produce 50 g of cells producing the recombinant protein? b) What is the oxygen demand in this process? c) For the cultivation of a wild E. coli not producing the recombinant protein, how different would the ammonia and oxygen demand be if the biomass yield remained at 0.48 gcel/gglyc ?
Production of a recombinant protein by E. coli is proportional to cell growth. Ammonia is used as a source of N and glucose as a source of C, under aerobic conditions. The recombinant protein has a general formula CH1.55N0.31O0.25 and the cell CH1.77N0.4900.24. The yield of biomass from glucose is 0.48 gcel/gglic, and the yield of recombinant protein from glucose is about 20% of that for biomass. a) How much ammonia is needed to produce 50 g of cells producing the recombinant protein? b) What is the oxygen demand in this process? c) For the cultivation of a wild E. coli not producing the recombinant protein, how different would the ammonia and oxygen demand be if the biomass yield remained at 0.48 gcel/gglyc ?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Production of a recombinant protein by E. coli is proportional to cell growth. Ammonia is used as a source of N and glucose as a source of C, under aerobic conditions. The recombinant protein has a general formula CH1.55N0.31O0.25 and the cell CH1.77N0.4900.24. The yield of biomass from glucose is 0.48 gcel/gglic, and the yield of recombinant protein from glucose is about 20% of that for biomass.
a) How much ammonia is needed to produce 50 g of cells producing the recombinant protein?
b) What is the oxygen demand in this process?
c) For the cultivation of a wild E. coli not producing the recombinant protein, how different would the ammonia and oxygen demand be if the biomass yield remained at 0.48 gcel/gglyc ?
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