Protein vaccines include only the parts (i.e. a protein) of a virus that best stimulate your immune system. This type of COVID-19 vaccine contains harmless S proteins. Once your immune system recognizes the S proteins, it creates antibodies and defensive white blood cells. If you later become infected with the COVID-19 virus, the antibodies will fight the virus. Assume that you work in a project for development of such vaccine against COVID-19. The vaccine contains S Protein and your job includes isolation of pure S Protein from the virus determination of charge on the protein (or pI) determination of molecular mass revealing three dimensional (3-D) structure of the protein How would you isolate (purify) the protein; suggest different techniques and explain the techniques. How would you determine the pI of the protein? Suggest techniques for determination of molecular mass.
Protein vaccines include only the parts (i.e. a protein) of a virus that best stimulate your immune system. This type of COVID-19 vaccine contains harmless S proteins. Once your immune system recognizes the S proteins, it creates antibodies and defensive white blood cells. If you later become infected with the COVID-19 virus, the antibodies will fight the virus.
Assume that you work in a project for development of such vaccine against COVID-19. The vaccine contains S Protein and your job includes
- isolation of pure S Protein from the virus
- determination of charge on the protein (or pI)
- determination of molecular mass
- revealing three dimensional (3-D) structure of the protein
How would you isolate (purify) the protein; suggest different techniques and explain the techniques. How would you determine the pI of the protein? Suggest techniques for determination of molecular mass. How would you analyze the structure of the protein?
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