QUESTION 20 Careful TEM studies suggested that histone octamers get removed during DNA replication. This raised a question regarding what happens to the histone octamers after they get removed. Do the histone octamers get disassembled after removal, or do they remain intact and get added back onto the DNA without disassembly. The figure below outlines an experiment that researchers performed to address this question. In a single sentence, describe the conclusion that can be drawn from this experiment. Experiment Question: What happens to histones in eukaryotic DNA replication? Methods 1 Grow cells for several generations in a medium that contains amino acids labeled with a heavy isotope. 2 Transfer the cells to a medium that contains amino acids labeled with a light isotope. Results Change medium Replication Isolate octamers 3 Isolate histone octamers before and after replication... Spin 4 ...and subject them to density-L gradient centrifugation. 5 Newly synthesized octamers are less dense and thus will be higher in the tube. 6 Old octamers are dense and will move toward the bottom of the tube. Isolate octamers Spin
QUESTION 20 Careful TEM studies suggested that histone octamers get removed during DNA replication. This raised a question regarding what happens to the histone octamers after they get removed. Do the histone octamers get disassembled after removal, or do they remain intact and get added back onto the DNA without disassembly. The figure below outlines an experiment that researchers performed to address this question. In a single sentence, describe the conclusion that can be drawn from this experiment. Experiment Question: What happens to histones in eukaryotic DNA replication? Methods 1 Grow cells for several generations in a medium that contains amino acids labeled with a heavy isotope. 2 Transfer the cells to a medium that contains amino acids labeled with a light isotope. Results Change medium Replication Isolate octamers 3 Isolate histone octamers before and after replication... Spin 4 ...and subject them to density-L gradient centrifugation. 5 Newly synthesized octamers are less dense and thus will be higher in the tube. 6 Old octamers are dense and will move toward the bottom of the tube. Isolate octamers Spin
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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