Salicylic acid salts inhibit the catalytic action of a glutamate dehydrogenase. Determine the type of inhibition by graphical analysis of the Following data. Assume that the salicylate concentration remains constant and is 40 mM. Also calculate the KM for the substrate and the dissociation constant for the enzyme-inhibitor complex. [S] mM 1.5 2.0 3.0 4.0 8.0 16.0 Without salicylate Product (mmol/min) 0.21 0.25 0.28 0.33 0.44 0.4 With salicylate Product (mmol/min) 0.08 0.10 0.12 0.13 0.16 0.18

Chemistry & Chemical Reactivity
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ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
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Chapter14: Chemical Kinetics: The Rates Of Chemical Reactions
Section14.7: Reaction Mechanisms
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Salicylic acid salts inhibit the catalytic action of a glutamate dehydrogenase. Determine the type of inhibition by graphical analysis of the Following data. Assume that the salicylate concentration remains constant and is 40 mM. Also calculate the KM for the substrate and the dissociation constant for the enzyme-inhibitor complex.

[S] mM 1.5 2.0 3.0 4.0 8.0 16.0
Without salicylate Product (mmol/min) 0.21 0.25 0.28 0.33 0.44 0.4
With salicylate Product (mmol/min) 0.08 0.10 0.12 0.13 0.16 0.18

 

 

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