Table A: Preparing 320 μL Glucose Standards Tube Label Approx. [Glucose] Standard (mg/mL) Std-1 0.000 Std-2 0.250 Std-3 0.500 Std-4 0.750 Std-5 1.000 Std-6 1.250 Volume of stock glucose if 2.00 mg/mL 0.000 με Volume of Milli-Q Water 320 με Actual Standard [Glucose] (mg/mL)
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Fill in the volumes required to make the standard glucose solutions of various conentrations provided. provide calculations as well.
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- Data for standard glucose concentrations Glucose concentration (mm) 0 0.5 1 1.5 -~~ 2 2.5 A Data for plasma samples B Patient Timepoint (hours) C D E 0 0.5 1 2 0 0.5 1 2 0 0.5 1 2 1. Draw a standard curve of absorbance at 420nm against glucose concentration in mmoles/L. We recommend you use Excel or a similar programme to insert the graph Alternatively, you can draw by hand on graph paper and insert a photo of the graph. OON 0 0.5 1 Absorbance 1 0.000 0.477 0.947 1.234 1.733 2.160 2 0 0.5 1 Absorbance Absorbance 2 0.000 0.482 0.941 1.236 1.721 2.276 Absorbance Glucose concentration Glucose concentration Abs 1 Abs 2 Average from graph (mM) in plasma (mm) 0.635 0.632 0.629 1.368 1.370 1.369 1.382 1.388 1.385 1.151 1.147 1.149 0.566 0.844 0.869 0.772 0.395 0.397 0.399 0.850 0.858 0.854 0.738 0.744 0.741 0.539 0.531 0.535 0.776 0.780 0.778 1.475 1.481 1.478 1.446 1.444 1.445 1.302 1.296 1.299 0.560 0.563 0.848 0.846 0.871 0.870 0.774 0.773 Average 0.000 0.480 0.944 1.235 1.727 2.218 0.440…Composition of Cell Lysis Buffer Required Concentration of Reagent Molecular Weight Volume or Weight of Reagents Needed 40 mM Tris HCl 157.59 20 mM Acetic acid 60.05 1 mM EDTA 292.24 10 mM MgCl2 from 25mM stock 95.21 5% SDS 288.37 ddH2O 18.02Materials - 2021FA-CHM-1O X Bb 4427577 d-fleet02-xythos.content.blackboardcdn.com/6086c260d7e8f/4427577?X-Blackboard-Expiration=1633143600000&X-Blackboard-Sig 6 / 9 100% Exercise 1: Standard Curve for Protein Measurements: A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples and spectrophotometer: following measurements were obtained using a Protein Concentration (mg/ml) Absorbance (A) 0.26 0.098 0.56 0.213 0.383 0.84 1.12 0.473 1.40 0.527 TASKS: 1. Enter the data into Excel - the…
- Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL XUsing G-25 Sephadex beads (Fractionation range 1000-5000) KD, a sample of Vitamin B12 (MW ~1500 KD) will be: Partially included Fully excluded Fully included
- What is the approximate rate of change of A340 measured? i.e. ΔA340 / min = __________ What rate of change of A340 would you predict if 30 ml of the ADH solution was tested in the same way (i.e. half the amount of protein) ? i.e. ΔA340 / min = __________ What rate of change of A340 would you predict if 60 ml of a 0.5 μM ADH solution was tested in the same way? i.e. ΔA340 / min = __________ As well as writing your answers, explain your reasoning.Insulin Lispro subcutaneously for a patient weight 80 kg for 1 month of treatment. Daily dose of insulin is 1 Unit/kg and 60% in short acting, given 4 times per day; ampoules in form of personal injectors (300 Units/3ml). (I would like to figure out the number of personal injector needed for 1 month.) My answer: Daily dose (DD) for a 80 kg patient = 80 x 1 Unit = 80 U 60% is short acting, so DD = 60% of 80 U = (0.6 × 80) U = 48 U Given 4 times per day, so for a single dose = 48 U/4 = 12 U For 1 month (30 days), 30 x 48U = 1440 U Rp. Insulin lispro in ampoules personal injectors 300 Units/3 ml No. ??? (my teacher write is 10 but I don't understand why) Sig. Inject subcutaneously 12 Unit (0.12 ml) before each meal, 4 times a day for 1 month.1&1/2 tsp po BID x 14 days #QS How many milliliters should the pharmacy dispense?
- calculate the drop rate of an IV if 1000mL of a 5% glucose solution is to be administered over a period of a 8h and the drop factor is 20 gtt/mLMatch the number in the image with the correct name for the lab supply 1 2 ♡ AGEN QIAGEN QIAGEN QIAC RIVCEM БИЗӘДІС RNeasy Mini Kit (50) Cat. No. 74104 Store at room temperature (15-25°C) WEZAIO QIAGEN 2 GDIVERM QIAGL K (20) QIAGL умени DAIO QIAG GAGEN GIVCEN QIAGEN QIAGEN ИЗОМО QIAGEN NEA QIAGEN QIAGEN N QIAGEN QIAGE Buffer W AGEN Lysis buffer 45 m Buffer RPE Wash buffer 11 ml concentrate to ustan 5 m Butter R U 3 clear collection tube centrifuge tube pink column (with filter) PhaseLock tube pink column (with filter) inside a clear collection tube <