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- A culture of Staphylococcus is diluted as follows:(1) 20mL are added to 80mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-2 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?When bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times with the loop?I AM TRYING TO IDENTIFY THIS UNKNOWN. IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. I believe it is one of the following: 1) S. pyo. 2)S. agal . 3)S.pneu. 4)E. faecalis 5)S. aureus 6)S epi. 7)S. sapro. 8)M. luteus Please let me know which test will i need out of the table to justify your reason of picking up the unknown and also how that test justifies it? what characteristics of that test made you pick the unknown?
- Why is washing with Hank’s balanced salt solution with 0.5% antibiotic-antimycotic done for Splenocyte Preparation?You are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flaskI AM TRYING TO IDENTIFY THIS UNKNOWN. ***IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. ***IMAGE 2 HAS THREE TESTS CONDUCTED ON IT, OXIDASE, BACITRACIN AND NOVOBIOCIN. I believe it is one of the following: 1) S. pyo. 2)S. agal . 3)S.pneu. 4)E. faecalis 5)S. aureus 6)S epi. 7)S. sapro. 8)M. luteus Please let me know which one of the above is thge unknown. You have pictures of the unkown on Catalase and Blood Agar and three test conducted on Oxidase, Bacitracin and Novobiocin. Give reasoning as why you think it will be one of the above. Explaing the characteristics and what made you deicide it?
- You mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do not know if you grew staph epidermis or E. coli. You found that the organism growing on the mannitol salt agar remained red after incubation. It is most likely that the organism is E.coli. a) True b) FalseAbigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?Suppose you spilled two cultures of Salmonella typhimur-ium (each containing 100,000 cells) on your lab bench. You im-mediately applied the same disinfectant to both cultures at thesame time. One culture had been freshly grown for 36 hours andthe other culture is 2 weeks old. If the kill ing rate of the disin-fectant is 90% per minute, do you think that microbes in bothcultures will be completely killed after 6 minutes? Why or whynot?
- I AM TRYING TO IDENTIFY THIS UNKNOWN. ***IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. ***IMAGE 2 HAS TWO TESTS CONDUCTED ON IT, BACITRACIN AND PYR I believe it is one of the following: 1) S. pyo. 2)S. agal . 3)S.pneu. 4)E. faecalis 5)S. aureus 6)S epi. 7)S. sapro. 8)M. luteus Please let me know which one of the above is thge unknown. You have pictures of the unkown on Catalase and Blood Agar and three test conducted on Bacitracin and PYR. Give reasoning as why you think it will be one of the above. Explaing the characteristics and what made you deicide it?The student advises using the same selection procedures and inoculating a 5 mL overnight culture with the transformed cells instead of searching for transformed coli on LB agar plates in order to save time. Why isn't this a wise decision?Since there are still bacteria left in pasteurized milk, why is it not routinely sterilized instead so that everything is killed?