What are the characteristics of an effective indicator organism? Write down some examples. Suppose you filtered a sample and found the Fecal Coliform count to be 20 CFU/100ml but the Total Coliform count was 0 CFU/100ml, do you think this result is correct? If so, why/why not?
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What are the characteristics of an effective indicator organism? Write down some examples. Suppose you filtered a sample and found the Fecal Coliform count to be 20 CFU/100ml but the Total Coliform count was 0 CFU/100ml, do you think this result is correct? If so, why/why not?
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- Where do you expect to see the highest number of CFU on a plate: Select one: a. When pasteurized milk is inoculated at dilution of 10-8 b. When pasteurized milk is inoculated at a dilution of 10-2 c. When raw milk is inoculated at a dilution of 10- d. When raw milk is inoculated at a dilution of 10-8 e. When raw milk is inoculated directly at a dilution of 10-2Provide one sample of any feed ingredient for non-ruminants with an appropriate description of its nutritive analysis and its characteristics using the format given below. Table 10. Format in the submission of non-ruminant feed sample with corresponding nutritive values. Common Name: Scientific Name: Source: Nutritive Analysis DM (%): СР (%): CF (%): Са (%): Avail P (%): Total P (%): ME Swine Kcal/kg: ME Poultry Kcal/kg: TDN Ruminant (%): Lysine (%): Methionine (%): Meth + Cystine (%):The Michaelis-Menten constant can be determined using the Lineweaver-Burk plot and is equal to twice the Vmax. can be determined using the Lineweaver-Burk plot and is equal to the substrate concentration at Vmax/2. can be determined using the Lineweaver-Burk plot. O is equal to the substrate concentration at Vmax/2. O is equal to twice the Vmax
- If the inoculated lactose broth cube exhibits acid production but without gas formation, will you consider this as a negative or positive result? Explain Why is the MPN test qualitative rather than quantitative? Why is the MPN test qualitative rather than quantitative? Why is MPN test qualitative rather than quantitativeUsing a P-1000 micropipette, you transfer 800 microliter of ddH2O to a beaker and measure its weight. It turns out to be only 780 milligram; what is the percent error of this micropipette?Explain this example of the following water quality serial dilution. Note the dilution factors and how you would calculate #’s from the incubated plates. ……A serial dilution yielded TNTC in the 1:1 plate and the 1:10 plate, 50 cfu’s in the 1:100 plate and 3 cfu’s in the 1:1000 plate . What is the concentration (CFU)/ml of the raw sample? Is water acceptable for potable or Recreational use? Explain. When do you use filtration rather than serial dilution
- Explain this example of the following water quality serial dilution. Note the dilution factors and how you would calculate #’s from the incubated plates. ……A serial dilution yielded TNTC in the 1:1 plate and the 1:10 plate, 10 cfu’s in the 1:100 plate and 1 cfu’s in the 1:1000 plate . What is the concentration (CFU)/ml of the raw sample?The accompanying figure is from an electrophoresis experiment using SDS–PAGE. The left lane has the following standards: bovine serum albumin (MW 66,000), ovalbumin (MW45,000), glyceraldehyde 3-phosphate dehydrogenase (MW 36,000),carbonic anhydrase (MW 24,000), and trypsinogen (MW 20,000).The right lane is an unknown. Calculate the MW of the unknown.N. benthamiana will be infiltrated with a solution containing OD600=0.3 of each experimental Agro containing construct and OD600=0.1 of p19. Calculate the volume of cultures (V construct; V p19) needed according to the formulas: V construct = V final × 0.3/OD600; V p19 = V final × 0.1/OD600. One mL of infiltrate is often enough to complete a small experiment. How to plan your final volume accordingly?
- The non linear part of the Standard curve graph can be used for estimating the unknown sample protein concentration? True FalseCompute the cfu/g (lactic acid bacteria and coliforms) of these samples given the following values below. Show your solution. Media First Sampling First Sampling First Sampling Second Sampling Second Sampling Second Sampling 10-2 10-3 10-4 10-2 10-3 10-4 VRBA TNTC, TNTC 243, 232 24,22 23, 25 0, 0 0, 0 MRS 32, 40 0, 0 0, 0 TNTC, TNTC TNTC, TNTC 102, 120You were assigned to investigate a poor production problem in one of the aquaculture sites of the company that you are working for. The overall fish production is not making any profit. You made an investigation on every part of the operation and appears to be normal and standard protocols had been followed. You ordered a feed analysis and the data is presented below in terms of the essential amino acid profile of the diet (see attached file): a) Based on this dietary profile, describe the possible disease that could happen to the fish stock and elucidate the signs of this disease b) Also, discuss how this disease could cause losses in production operation c) Design and describe a solution to resolve this production problem.