What are the five main steps in DNA cut-and-paste transposition?

Q: What is linker scanning mutagenesis?

A: Answer: Introduction: Old-style approaches, containing linker-scanning mutagenesis includes…

Q: How can we monitor DNA denaturation?

A: Double-stranded DNA is the native secondary structure. The conversion of double-stranded DNA into…

Q: What is the name of the DNA duplication process? What is the main enzyme that participates in it?

A: Every living cell divides to form multiple cells. During this division process, there is another…

Q: What causes different linear DNA molecules to migrate at different speeds during electrophoresis?

A: In molecular biology many techniques are used for different purpose like PCR is used to generate…

Q: What is a poor transformation efficiency?

A: Transformation is a natural phenomenon mostly observe in many bacteria. It is dependent on the…

Q: What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that…

A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chain that is…

Q: What is the principle of next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: What is the mom method of pacaking DNA?

A: Answer: DNA : Deoxyribonuleic Acid acts as the genetic material which transfers the genetic…

Q: Why is it difficult in a single experiment to transfer a largenumber of genes to a recipient cell…

A: Gene transfer is the process of insertion of unrelated DNA into cells. There are three mechanisms of…

Q: What are recombinant proteins? How do bioreactors help in their production?

A: The study that involves the molecular level activities that occur in living beings is called…

Q: What are the basic steps involved in cloning foreign DNA into a vector?  How do you differentiate an…

A: Cloning Cloning refers to the making of identical copies. DNA cloning is a molecular biology…

Q: What is the difference between positive and negative supercoiling?

A: The DNA (Deoxyribonucleic acid) is the genetic material that is inherited by offspring over…

Q: What is the function of the homogenizing solution in DNA isolation? What are its components?

A: In the process of DNA isolation it is very important to breakdown or lyse the cell this allow the…

Q: What are the advantages of using a recombinant DNA product from a human source instead of a product…

A: GENETIC ENGINEERING:- It is a modification in genes that is done using biotechnology ( Recombinant…

Q: Why is ligase needed to make recombinant DNA? Whatwould be the immediate consequence in the cloning…

A: DNA replication is the process of copying the DNA of the cell during cell division. DNA ligase is an…

Q: How is recombinant DNA inserted into a genome using CRISPRediting?

A: The modern technology of genetics can be utilized to increase the genomes of various living…

Q: What is the principle of DNA isolation?

A: DNA isolation is a process through which the intact DNA of the organisms is extracted and used for…

Q: What are two factors that will determine the frequency at which a restriction endonuclease cleaves…

A: Restriction enzymes are the type of enzyme that is used to cleave the DNA during the molecular…

Q: what are similarities and differences between chain-termination and reversible terminator…

A: Gene sequencing  aids in the development of gene therapy, a form of treatment that replaces faulty…

Q: How to create  recombinant DNA molecules withplasmid vectors?

A: Recombinant DNA is the one which consist of autonomous replicating DNA segment along with inserted…

Q: What is restriction fragment length polymorphism and which method do we use to detect it?

A: Restrictions length polymorphism (RFLP) is a kind of polymorphism that outcomes from variety in the…

Q: What is the function of the chaotropic salt in DNA isolation?

A: These salts are strong protein denaturing agents.

Q: How does PCR minimize the problems associated with degraded DNA? What factors can cause DNA to…

A: PCR is Polymerase chain reaction.It is a method widely used to rapidly make millions to billions of…

Q: What are the three main steps in procedures of transformation?

A: Bacterial transformation is a horizontal gene transfer wherein certain bacteria called competent…

Q: What is the cause of an extra band in a DNA ladder in agarose gel electrophoresis?

A: The following can lead to the formation of an extra band in the DNA ladder lane - 1. Contamination -…

Q: In DNA cloning, why do we need to check colonies for insert after transformation?

A: Gene is the basic unit of heredity. All living organisms contain certain nucleic acids as their…

Q: what is   Inverse PCR ?

A: PCR which stands for polymerization chain reaction is an analytic technique which is used to amplify…

Q: How does the use of restriction endonucleases of different specificities aid in the sequencing of…

A: Restriction endonucleases of different specificities cleave DNA at specific sites.

Q: What are major principles of DNA cloning?

A: DNA cloning refers to the production of large number of identical DNA from a single DNA. DNA cloning…

Q: How is genomic DNA prepared for shotgun sequencing?

A: Genomic DNA prepared for shotgun sequencing as follows :  1. ligating random genomic DNA fragments…

Q: What is the difference between the first steps of DNA sequencing to PCR in terms of the number of…

A: PCR is expanded as a polymerase chain reaction. It is a technique that is used widely for the…

Q: What is a good transformation efficiency?

A: Transformation is a common phenomenon in microbes like bacteria and results in genetic alteration of…

Q: What is the difference between a map-based approach to sequencing a whole genome and a whole-genome…

A: Deoxyribonucleic acid (DNA) is a double stranded helical genetic material containing thousands of…

Q: What is the role of a primer in DNA replication? What would happen if you forgot to add a primer in…

A: DNA replication is the process in which a parent DNA is separated and two new strands are formed…

Q: What is a recombinant microorganism? Discuss examples.

A: Recombination – this is a process used to produce a different combination of alleles by breaking the…

Q: How many copies of a target DNA molecule are made after 30 cycles of PCR?

A: The polymerase chain reaction is a widely used technique for the amplification of DNA. Here, the…

Q: what are Several Limitations of positional cloning?

A: Positional cloning is one of the laboratory technique of gene identification. In this method, gene…

Q: Why is a primer needed at each end of the DNA segment beingamplified by PCR?

A: Primer - Primer is a kind of short nucleotide sequence which is used as a starting point in the…

Q: What is the transformation factor?

A: The process in which the genetic material formed of the naked DNA (deoxyribonucleic acid), which is…

Q: What is a plasmid? What are the minimum 3 components that a recombinant plasmid used for genetic…

A: Biotechnology is innovation that uses natural frameworks, living beings or parts of this to create…

Q: What is the role of detergent and salt in the DNA extraction process

A: The three basic steps of DNA extraction are 1) lysis, 2) precipitation, and 3) purification.

Q: what is the purpose of calcium chloride in the transformation process

A: Calcium chloride transformation is a laboratory technique in prokaryotic (bacterial) cell biology.…

Genetic Evolution

Sexual reproduction produces distinct population variations. Heredity determines the traits and qualities to be passed down to the progeny. Over time, this variation within-species is also affected by the process of human evolution. The study of how genetic variation leads to evolutionary changes is known as genetic evolution. If a trait helps an organism to survive and reproduce, it is more likely to be passed down the generations. It is rare that such traits result in a completely distinct set of traits in descendants than the ancestral population; this can result in the emergence of a new species.