When a gene is duplicated resulting in two copies, there are three possible fates for the duplicate copy Sort each item to the appropriate bin. Potential to generate a new phenotype Most likely outcome for duplicated genes Pseudogene (Nonfunctionalization) 4 Both gene copies are required to carry out the functions of the original gene One gene copy is inactivated due to deleterious mutations Subfunctionalization Most interesting evolutionary outcome for duplicated genes Least likely outcome for duplicated genes Reset Help Neofunctionalization
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- A+ 70°F, a cell cultue is able to graw and divide farever, but after a new mutation is found in the čeus, and the cells are at 99°F, mey Stop dividing after a few generahons.what * 16 ikery disiupted by the mutation for the cells to stop dividing after a few gener ution when Compared to the 70°F when they diide and qrow indefinitely! grown they aure 95°F grown atResearchers have identified a gene in humans that (when mutant)causes severe dwarfism and mental impairment. This disorder isinherited in an autosomal recessive manner, and the mutant alleleis known to be a loss-of-function mutation. The same gene hasbeen found in mice, although a mutant version of the gene has notbeen discovered in mice. To develop drugs and an effective therapyto treat this disorder in humans, it would be experimentallyuseful to have a mouse model. In other words, it would be desirableto develop a strain of mice that carry the mutant allele in thehomozygous condition. Experimentally, how would you developsuch a strain?The cells from a person’s malignant tumor were subjected to insitu hybridization using a probe that recognizes a unique sequenceon chromosome 14. The probe was detected only once in each ofthe cells. Explain this result, and speculate on its significance withregard to the malignant characteristics of these cells.
- A research paper published in the summer of 2012presented a method to obtain the whole-genome sequence of a fetus without any invasive procedure suchas amniocentesis that could on rare occasions causemiscarriage. This new technique is based on the factthat some fetal cells leak into the mother’s bloodstream and then break down, releasing their DNA.Assume that exactly 10% of the DNA fragments inthe mother’s blood serum come from the fetus, whilethe remaining 90% of the DNA fragments in the serum come from the mother’s genome.The investigators collected cell-free DNA from apregnant woman’s bloodstream and subjected it to anadvanced high-throughput sequencing method. Thetable at the end of this problem looks at seven unlinked loci; the number of reads of particular alleles(identified by Greek letters) are shown. You shouldassume for the sake of simplicity that all numericaldifferences are statistically significant (even thoughactual data are never this clean).a. Determine whether each…When 1 million cells of a culture of haploid yeastcarrying a met− auxotrophic mutation were plated onpetri plates lacking methionine (Met), five coloniesgrew. You would expect cells in which the originalmet− mutation was reversed (by a base change back tothe original sequence) would grow on the media lackingmethionine, but some of these apparent reversions couldbe due to a mutation in a different gene that somehowsuppresses the original met− mutations. How wouldyou be able to determine if the mutations in your fivecolonies were due either to a precise reversion of theoriginal met− mutation or to the generation of a suppressor mutation in a gene on another chromosome?A rare dominant mutation expressed at birth was studiedin humans. Records showed that six cases were discoveredin 40,000 live births. Family histories revealed that in twocases, the mutation was already present in one of the parents.Calculate the spontaneous mutation rate for this mutation.What are some underlying assumptions that may affect ourconclusions?
- Roman 16px : 1.0pt : BIUS A A X2 x E tion of chromosomes did not occur berore cytoknESIS, 1/2 the # of chromsomes or 1 of each b. If the situation in part a occurred, would the new cells be viable? Explain. no, it wouldnt have the DNA OP 10. The S phase stands for synthesis, which means to make or build something more complex out of simpler parts. Scientists know that during the S phase DNA is being made in the nucleus of the cell. Why do you think the cell needs to make more DNA at this time in the cell cycle? 11. Refer to Model 1. The chromosomes that are shaped like "X" (made of two sister chromatids) have double the amount of DNA than the chromosomes that are shaped like "I." During what phase of the cell cycle do you think the chromosomes are replicated (copied)? POGIL Activities for High School Biology 2. hpLet’s suppose an X-linked gene in mice exists as two alleles, whichwe will call B and b. X-chromosome inactivation, a process inwhich one X chromosome is turned off, occurs in the somatic cellsof female mammals . Allele B encodes an mRNA that is 900 nucleotides long, whereas allele b contains a small deletion that shortens the mRNA to a length of 825 nucleotides.Draw the expected Northern blot that will be obtained usingmRNA isolated from somatic tissue of the followingmice:Lane 1. mRNA from an XbY male mouseLane 2. mRNA from an XbXb female mouseLane 3. mRNA from an XBXb female mouse.Note: The sample taken from the female mouse is not from aclone of cells. It is from a tissue sample, like the one shown.explain the statement Duplications Provide Extra Gene CopiesThat Can Acquire New Functions
- Researchers have successfully used gene therapy toameliorate some human genetic diseases by adding anormal gene copy to cells whose genomes originallyhad only nonfunctional mutant copies of that gene.For example, a form of blindness due to the lack of asingle protein called RPE65 has been reversed byintroduction of a normal RPE65 gene to cells of theretina of adults.a. The success of this gene therapy approach providesus with clues about the role of the RPE65 proteinin the retina. Do you think that RPE65 is neededfor the proper development of the human eye?b. Can you see a potential difficulty in applying this genetherapy approach for diseases like microcephaly?. You receive four strains of yeast in the mail, and theaccompanying instructions state that each strain contains a single copy of transgene A. You grow the fourstrains and determine that only three strains expressthe protein product of transgene A. Further analysisreveals that transgene A is located at a different position in the yeast genome in each of the four strains.Provide a hypothesis to explain this resultThe molecular mechanism of SCE is similar to homologousrecombination between homologs except that the two segmentsof DNA are sister chromatids instead of homologous chromatids.If branch migration occurs during SCE, will a heteroduplex beformed? Explain why or why not. Can gene conversion occurduring sister chromatid exchange?