Yeast cells has been cultured on glucose (Table 1). The growth data follows the Monod Equation Table 1: Growth data for yeast cells Glucose concentration (mg/L) Specific growth rate (h*¹) Observed yield, Y'x/s (g/g) 7 10 15 40 200 0.066 0.088 0.12 0.2 0.304 0.294 0.330 0.364 0.419 0.451 1000 0.340 0.458 a) Calculate the maximum specific growth rate (max) and the substrate constant (Ks)

Curren'S Math For Meds: Dosages & Sol
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ISBN:9781305143531
Author:CURREN
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Chapter10: Reconstitution Of Powdered Drugs
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Yeast cells has been cultured on glucose (Table 1). The growth data follows the Monod Equation
Table 1: Growth data for yeast cells
Glucose concentration (mg/L)
7
10
15 40
200
1000
Specific growth rate (h*¹)
0.066
0.088
0.12
0.294 0.330 0.364 0.419 0.451
0.2 0.304 0.340
0.458
Observed yield, Y'x/s (g/g)
a) Calculate the maximum specific growth rate (max) and the substrate constant (Ks)
b) Estimate the true yield Y'x/s and the maintainance coefficient ms
c) Calculate the doubling time at a concentration of 15 mg/L glucose
d) Calculate the substrate consumption rate (rs) assuming a substrate concentration of 40
mg/L and a biomass concentration of 100 mg/L
Transcribed Image Text:Yeast cells has been cultured on glucose (Table 1). The growth data follows the Monod Equation Table 1: Growth data for yeast cells Glucose concentration (mg/L) 7 10 15 40 200 1000 Specific growth rate (h*¹) 0.066 0.088 0.12 0.294 0.330 0.364 0.419 0.451 0.2 0.304 0.340 0.458 Observed yield, Y'x/s (g/g) a) Calculate the maximum specific growth rate (max) and the substrate constant (Ks) b) Estimate the true yield Y'x/s and the maintainance coefficient ms c) Calculate the doubling time at a concentration of 15 mg/L glucose d) Calculate the substrate consumption rate (rs) assuming a substrate concentration of 40 mg/L and a biomass concentration of 100 mg/L
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