Lab Report #2 UPDATED (other one became blank)

18YW9TJVEYLM/edit of Enzyme Inquiry Questions mat Tools Add-ons Help Last edit was 3 days ago Normal text Calibri 13.5 BIUA GD 1. 3 . 4 E E E | Enzymes Narme: Directions: Each series of diagrams is designed to provide you with information about enzymes and chemical reactions. Please highlight, color, and/or BOLD your responses to make them easier to read! (6.5 pts/ question) Reactants Diagrams 1-4. Chemical reactions involve changes in chemical bonds. A CHEMICAL REACTION Product (left side of the arrow) (right side of the arrow) reactants - products NH, + HCI > NH,CI chemical reaction starts with one set 4. of molecules and turns them into Producte- he Reactante.the starting molecules 2H, + O, - 2H,0 final molecules something new by forming or breaking 6CO, + 6H,O - C,HO, + 60, chemical bonds. Reactants (Substrates) Products Chemical change 1. Explain what the "reactants" are in a chemical reaction. 2. What does the "arrow" of a chemical reaction represent? 3. What are the molecules…

m BIO101 Short EXAM First session X G Google om/moodle/mod/quiz/attempt.php?attempt%3D1313727&cmid%3D298536 e on the bookmarks bar. Import bookmarks now... AL BIOLOGY I/ ill courses GENERAL BIOLOGY I BIO101 Short EXAM First sess Time left 0:21:42 If an enzyme in solution is saturated with its substrate, the most effective way to obtain a higher reaction rate is to: O a. add more substrate O b. heat the solution to 95°C O c. add more of the enzyme O d. All of the options are correct O e. add a noncompetitive inhibitor Next page vity Jump to.. ents 51°F Light rair

Topic: Co-Enzyme Q10   Question: What does it mean when asked to explain the evidence behind medicinal chemistry of the CAM agent, and if the chemical structures relate to it's effects.

I. OBJECTIVES Solve problems involving reaction mechanism in chemical kinetics Cite and differentiate types of catalysts II. GENERAL INSTRUCTIONS Answer the following items. 1. Give an example of a catalyst and its importance. Examples in the material (slideshow and video) will not be accepted. 2. Identify the enzymes in the Tricarboxylic Acid Cycle below. Acetyl-CoA COASH -co0 HO-C-CDO HE-coo Oxalcacetate NAH Citrate 000- 000- co0 -coo -coo L-Malate coo cis-Aconitate Tricarboxylic Acid Cycle coo Fumarate Isocitrate NADH Succinate COASH a-Ketoglutarate SCal Succinyl-CoA

d Quiz: Apartic X + .com/courses/42082/assignments/271401 Guidelines h the lecture video below and take notes. Complete the embedded quiz by Due Date.. Technical Support a help using Kaltura Quizzes? If so, please review the following guide: altura Video Quizzes - How to Take a Quize Atanzavir inhibits HIV-protease by mimicking the transition state of the hydrolysis reaction true false Next evious MacBook Pro Q Search Secure Search ( ) * & 2$ %3D 5 6 7 9

OCPS Das X ← → CO PS Login OCPS + Orders - X Performance Matters Question 12 of 16 Rate of reaction Previous Performa X olaocps.performancematters.com/ola/ola.jsp?clientcode=flocps# Join a Game - Quizizz K! Play Kahoot! - Enter... V C Educator X 19 Exam: 01 × Plans for X Submit Test Figure 1. Enzyme Rate of Reaction X = point of saturation The diagram below shows the relationship between substrate concentration and reaction rate. Dashboard ↑ Increasing concentration does not affect reaction rate AP EL U.S. Citize X F https://sex Performance Matte... S FLVS Login Welcome, Asheley! 02.Cl.Biology.CRM3.2_2023 4JypvK8X X + OT QMy Quizzes - Quizizz Athletic Clearance Substrate concentration Based on the graph, which of the following describes why continually increasing substrate does not lead to a continuous increase in reaction rate?

Review method used to increase the solubility of a drug under the following headings co solvents PH control solubilization complexation can you provide detail answers please

Task No. 2: Cytochrome Completion Directions: Supply the missing information. Amino Acid Sequences of in Cytochrome-c ANIMAL AMINO ACID SEQUENCE 1. HORSE 2. CHICKEN 3. FROG 4. HUMAN 5. SHARK 6. RAT 7. PIG 8. DOG 9. SNAKE 10. FLY

O DOS Lab_Audio_Script x A Concept Check-large bi x Untitled document - Go x * VHL Central | Dashboar x Quiz: Concept Check-la x b My Questions | bartleby x + A canvas.northseattle.edu/courses/2086259/quizzes/5876542/take Account Dashboard Courses Groups The reaction at the top, which is occurring where the blue circles are, is a [ Select ] reaction. Calendar The molecule on the left of the upper image is [ Select ] and the Inbox molecule on the right is [ Select ] History The molecule at the bottom is a(n) [ Select ] and Help [ Select ] dissolve in water. O v i 9:22

instructions: add the drawings to these given answers please. its urgent answers are given as follows A. The tetrahedral intermediate attacks the carbonyl carbon of the peptide bond, cleaving it and forming a new peptide bond with the nitrogen of aspartate. This process is repeated for each peptide bond in the substrate, resulting in the formation of the product and free enzyme. In this example, the product is a dipeptide and free enzyme. In addition to forming a tetrahedral intermediate, the catalytic mechanism of papain involves general acid-base catalysis through His57 in Asn152. The amino group in Asn152 deprotonates the hydroxyl side chain in Phe153, which is stabilized by hydrogen bonding. This deprotonation facilitates nucleophilic attack at the carbonyl carbon of the peptide bond in the substrate, resulting in a tetrahedral intermediate. His57 also protonates Asp102, stabilizing it as a general base to facilitate formation of the tetrahedral intermediate. The nucleophilic…

Answered uses and applications 1 Which material is a significant source of carbohydrates? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. 寒 XIX plant stems b sunflower oil hair and fingernails fat-soluble vitamins Ar

O School District of Palm E Cindy Mejias - [Template x Performance Matters | C x E Cindy Mejias - The Six K x K Cindy Mejias-[Termplate x * Course Hero A olapalmbeach.performancematters.com/ola/ola.jsp?clientCode=flPalmBeach# D SDPBC Bookmarks Performance Matters Welcome, Cindy Mejias! Connection Status: All Question 3 of 18 - Bio_U08_USA_FY21 Question: 1-3 A certain plant tissue, typically found in the stem and in the hard outer covering of seeds, contains very thick, rigid cells. Which of the following correctly identifies this tissue and its function? meristem tissue; gamete formation O vascular tissue; production of sugar ground tissue; transportation of materials O dermal tissue; protection against damage Previous Pause O Sign on

Module 5 Week 1 Assignment: Enzymes. This is a group assignment. You must work with a partner. Please be sure to have joined a group in Canvas and then upload one file for the group. The following kinetic data were obtained for an enzyme in the absence of inhibitor (1) or in the presence of two different inhibitors (2 and 3). The Enzyme concentration was the same in all of the experiments. Graph these data as a Lineweaver-Burke plot. Enzyme mM Inhibitor 2 [S] 1 Inhibitor 3 2 4 8 12 Enzyme (1) 12 20 a. Paste a photo of your Linewaver-Burke plot here. Plot all three lines on one graph. b. Using the date, fill in the following table; 29 35 40 v (umol/ml *sec) Inhibitor (2) 4.3 8 14 21 26 Vmax Km Apparent Vmax Apparent Km Type of inhibition Apparent Vmax Apparent Km Type of inhibition Inhibitor (3) 5.5 9 13 16 18 Click or tap here to enter text. Click or tap here to enter text. Click or tap here to enter text. Click or tap here to enter text. Click or tap here to enter text. Click or tap…

Questions 1. What are the effects of pH and temperature to catalase? What is the optimum pH and optimum temperature for catalase? 2. Explain why the rate of reaction initially increases with increase in temperature then gradually declines as the temperature is further increased. 3. Is the rate of enzymatic reaction always directly dependent on enzyme concentration? Explain. 4. Explain the effect of substrate concentration on enzyme activity. 5. What is the effect of CuSO, on the enzymatic activity of catalase? 6. Is CuSO4 an activator or inhibitor? If it is an inhibitor, what kind of inhibitor is it?

Activity 1: Practice Problems 1. In humans, phenylketonuria (PKU) is a disease caused by an enzyme deficiency at step A in the following simplified reaction sequence, and alkaptonuria (AKU) is due to an enzyme deficiency in one of the steps summarized as step B: A B phenylalanine tyrosine CO₂ + H₂O A person with PKU marries a person with AKU. What phenotypes do you expect for their children? wildtype O PKU O AKU O Both PKU and AKU

Separation of Amino Acids by Thin Layer Chromatography Lab Questions 1. Describe in detail Thin layer chromatographic experiment. Example: the theory behind it, how youwould prepare the materials to spot on the plate with different mobile amino acids and unknown andhow TLC Plate is developed and the reasoning behind which solvent/ solvent mixture should be used,along how to correctly identify of the unknown. 2. Calculate the Rf value if a solute travelled 5 cm from the base spot and the solvent front is 10 cmfrom the origin? 3. In a TLC experiment using a 70:30 mixture of Petroleum ether and ethyl acetate, a student noted thedevelopment of spots in the origin, what can you suggest about this observation?

2 W 1 Z MacBook Air PRE-LAB ASSIGNMENT Enzyme - Functional Analysis Instructions: Read the Introduction section of the lab and watch the following video: https://www.youtube.com/watch?v=V6wHXtO9kIA. Then complete the questions below. 1. Define Enzymes. What type of macromolecules are enzymes? 2. What are two advantages of having enzyme-catalyzed chemical reactions in living cells? reaction? 3. What is a substrate? Where on an enzyme does the substrate specifically bind to during a chemical in each of following: 4. When an enzymatic reaction is in progress, do you expect to see an increase, decrease or no change (i) substrate (ii) product (iii) enzyme 5. How can you measure enzyme activity? 77

Case Study: Case Study: Catalase Activity Catalase H,O, 0 + O2 (2) Catalase is an enzyme that converts hydrogen peroxide (H,O) to oxygen and water. An experiment investigated the effect of temperature on the rate of the catalase reaction Small (10 cm) test tubes were used for the reactions, each containing 0.5 cm of enzyme and 4 cm of hydrogen peroxide. Reaction rates were assessed at four temperatures (10°C, 20°C, 30'C. and 60 C). For each temperature, there were two reaction tubes (e.g. tubes 1 and 2 were both kept at 10°C). The height of oxygen bubbles present after one minute of 10°C 20°C 30°C 60°C Height of охудen bubbles 4 cm H,02+ 0.5 cm erzyme reaction was used as a measure of the reaction rate: a laster reaction rate produced more bubbles. The entire experiment, involving eight tubes, was repeated on two separate days. Tubes Tubes Tubes 1 & 2 Tubes 3 & 4 5 & 6 7 & 8 1. What is the purpose of this experiment? inrestrgatee effect of demperalure on the rate ot he 2. Write a…

Question: what is the action of acid and alkali on the enzyme in saliva? Acid: Alkali:   (Procedure and data are already given)

Answers should be specific and mention pathway details from the book. PLEASE ANSWER ALL. im very confused, will upvote. Briefly describe the difference between a reversible and irreversible reaction in a metabolic pathway.

Summaries of two clinical case studies follow. For each case determine which enzyme isdefective and designate the appropriate treatment, from the lists provided at the end of theproblem. Justify your choices. Answer the questions contained in each case study. 15 marksCase A The patient complains of painful muscle cramps when performing strenuous physicalexercise but has no other symptoms. A muscle biopsy indicates a muscle glycogen concentrationmuch higher than normal. Why does glycogen accumulate?Case B The patient is lethargic, her liver is enlarged, and a biopsy of the liver shows largeamounts of excess glycogen. She also has a lower than normal blood glucose level. What is thereason for the low blood glucose in this patient?

Question: Asystole For this disease pathology, please provide the following information: What causes this disorder (pathology)? Are there any threats to life risks associated with this disease that you should watch out for, and why? (Not all terms will have life-threatening conditions associated with them) What would you expect to see in the lab or diagnostic results? What are the most common treatments?

Kindly answer all questions please, reealy need help. Pick only the correct letter, no explanations needed. Letter only. 1. The human body consists of several molecules that aid in various bodily processes. What biological polymer functions as a catalyst, speeding up the reaction without being consumed? A. Enzyme B. Phospholipid C. Carbohydrate D. Protein 2. The enzyme can be inactive which is called an apoenzyme, or active which is called a holoenzyme. Determine what makes an apoenzyme turn into a holoenzyme A. Cofactor B. Coenzyme C. Substrate D. Active Site 3. Concentration Effect : Substrate Concentration :: Inhibitor’s Effect : _____________________ A. Competitive Inhibition B. Noncompetitive Inhibition C. Enzyme Concentration D. Water Effects 4. There are different factors affecting enzyme activity. Determine what factor means that the enzymes require hydration to produce activity? A. Water Effect B. Activator's Effect C. Concentration Effect D. Inhibitor's Effect

Recommendations to improve on the efficacy of the enzymatic colorimetric method of determining the concentration of lipids in the blood.

What are the corresponding answers, respond briefly, site sources Enzyme; Triacylglycerol Lipase Enzyme Official Name (write N/A if not applicable)   Enzyme Official Number (4 digits)   Simple or Conjugated Enzyme (specify cofactor if applicable)   Type of Reaction Catalyzed     Substrate   Optimum pH   Optimum Temperature   Function/s   Disease (give 1 and describe briefly)         Enzyme: Urease Enzyme Official Name (write N/A if not applicable)   Enzyme Official Number (4 digits)   Simple or Conjugated Enzyme (specify cofactor if applicable)   Type of Reaction Catalyzed   Substrate   Optimum pH   Optimum Temperature   Function/s   Disease (give 1 and describe briefly)

Materials - 2021FA-CHM-1O X Bb 4427577 d-fleet02-xythos.content.blackboardcdn.com/6086c260d7e8f/4427577?X-Blackboard-Expiration=1633143600000&X-Blackboard-Sig 6 / 9 100% Exercise 1: Standard Curve for Protein Measurements: A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples and spectrophotometer: following measurements were obtained using a Protein Concentration (mg/ml) Absorbance (A) 0.26 0.098 0.56 0.213 0.383 0.84 1.12 0.473 1.40 0.527 TASKS: 1. Enter the data into Excel - the…

Active Raw Actual Yield Formulation Dosage Form Packaging Ingredient Materials 2 kgs calcium oxide and Calcium 1.75kgs 16omg per Oral 100mL per carbonate calcium 10mL Suspension bottle 1.75 kg H,O carbonate suspension Please fill the cells in the second column of the table below with correct information. Thank you! IA. RAW MATERIAL SOURCING Source (natural/synthetic) of RAW MATERIALS Method of separation/chemical reaction (with brief process) IB. ACTIVE INGREDIENT INFORMATION Active Ingredient Chemical Name/Other Name Active Ingredient Classification (ex. Analgesic)

pic: 3.1 Discussion Forum с Student Po... X New Tab X ftccollege.instructure.com/courses/19318/discussion_topics/259958 Log In to Canvas Cengage eTextbook + Email | Microsoft 365 3.1 Discussion Forum What is Scatter Radiation and How is it Controlled? Type here to search Instructions 1. After reading chapter 9 from your textbook, you will be able to explain scatter radiation and how it is produced as a result of the attenuation of the x-ray beam by matter. You will also recognize the factors that influence its formation and how to minimize the fog that this radiation causes on radiographs. NCCT Note: Please read the instructions provided in the following links before submitting your entry: . • Participation Rules . Online Etiquette Rules B LM 2. For your participation in the forum, answer the following questions: a. Fog on a radiograph becomes objectionable when one body part size is larger than another. Based on what you read, explain which body part will be objectionable? b. How is…

Submit a 3-page Word document on enzyme-catalyzed reactions as described by the Michaelis-Menten kinetics. Practice proper citation of your references.

Case study: A child was admitted to the hospital with vomiting, partial paralysis and impaired brain function. The blood test indicated unusually high levels of pyruvate and a- ketogluterate in the blood. The urine of this patient smells of maple syrup. Genetic analysis indicated no defect in any enzymes. What would you suspect about the diagnosis of this patient? Explain the basis of your diagnosis.

A biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure Total protein (mg) 20,000 5,000 4,000 Activity (units) 4,000,000 3,000,000 1,000,000 800,000 750,000 675,000 1. Crude extract 2. Precipitation (salt) 3. Precipitation (pH) 4. Ion-exchange chromatography 5. Affinity chromatography 6. Size-exclusion chromatography From the information given in the table, calculate the specific activity of the enzyme after each purification procedure. b. Which of the purification procedures used for this enzyme is the most effective (I.e. gives the greatest relative increase in purity)? Which of the purification procedures is least effective? d. Is there any indication based on the results calculated in part A that the enzyme after step 6 is now pure? What else could be done to estimate the purity of the enzyme preparation? 200 50 45 a. C.

Question: What's wrong with this patient? What therapy should be suggested for this disease? Prognosis in an Elderly Female, by V. Dimov, M.D., Reviewer: S. Randhawa, M.D.A 96-year-old African American female (AAF) was admitted from a nursing home with complaints of abdominal pain, nausea and vomiting (N/V), dizziness, confusion and double vision for 5 days. She was discharged from the hospital just 4 days ago. Digoxin was started during that previous hospitalization for control of tachycardia in atrial fibrillation. One day prior to discharge, digoxin level was 1.8 mg/mL and digoxin dose was decreased to 125 mcg PO Q 48 hr.Past medical history (PMH): Hypertension, atrial fibrillation, coronary artery disease, stroke, congestive heart failure.Medications: Metoprolol, digoxin, ASA (aspirin), lisinopril, furosemide (Lasix), Coumadin (warfarin), esomeprazole (Nexium).Physical examination: In pain, combative and confused.VSS.Chest: Occasional bibasilar crackles.Cardiovascular System: Clear…

student question 1. How do both Hemoglobin and S-adenosylmethionine synthetase form hydrophobic pockets? explain in detail. 2. how does the structure of S-adenosylmethionine synthetase make it resistant to heat denaturation and why, explain in detail