Egg Osmosis Using “Deshelled” Chicken Eggs
Martekka Crawford
India Williams
Desiree Counts
Mark Beckford
Biology 1611-Sec 348
Dr. M. Jones
September 17, 2015
Georgia Perimeter College- Decatur
Introduction
The osmolarity of a solution is the concentration measured of a solute. To determine the concentration of a solution, one must first understand what osmosis is and how it works. Osmosis is the spontaneous passage or diffusion of water or other solvents through a semipermeable membrane according to the Britannica Encyclopedia. The semipermeable membrane is one that blocks the passages of dissolved substances such as solutes. This process, in more recent times, provides a more accurate study of how water can diffuse across a cell membrane when water molecules have a high concentration to an area in which they have a low concentration. It was originally studied in 1877 by plant physiologist, Wilhelm, Pfeffer. (Britannica Encyclopedia 2015).
Osmosis is described in one of three ways when comparing more than one solution. The cell’s external and internal environment helps determine tonicity, which is defined as how the cell reacts to its environment. When the cell’s environment is equal in osmolarity to itself and there is no change, it is considered an isotonic solution. When the environment has a higher osmolarity, shrinkage occurs and it is considered a hypertonic solution. When the environment has a lower osmolarity, swellings occurs and it is considered hypotonic.
Cells and molecules in the environment are constantly moving and changing, for cells to function properly there is a need for equilibrium to be met. The size of the cell and the solution outside of the cell affects the rate of diffusion and osmosis in the cell. Cells are constantly trying to reach an equilibrium with the molecules and substances around it, which is why there are such terms as: hypertonic, hypotonic and isotonic. The procedures allowed testing of whether or not surface area or volume increased diffusion and how different substance control diffusion. Cells are constantly moving to reach equilibrium through diffusion and osmosis.
The purpose of this lab is to determine what is the concentration of the unknown solution along with what concentration would be more isotonic to the chicken egg?
As the lab introduction explains, osmosis is relatively permeable to water and will follow solutes. By instinct, the water will move from a more diluted solution to more of a concentrated solution. The products of the experiments concluded the physiological significance of osmosis by how cell membranes in the body are semipermeable meaning that only certain molecules can pass through it. When intracellular fluid and extracellular fluid are at equilibrium by non-penetrating and concentrated solutes, no net movement of water goes in and out of the cell. Furthermore, if the ECF changes in osmolality, then depending on the difference between the ECF and ICF will determine whether water moves in or out of the cell. This is important in the cell membrane as small differences in osmolarity correspond to large, rapid change in osmotic pressure, causing cells to gain or lose water. In sum, our body makes critical decisions in what molecules are allowed to penetrate the cell membrane and make sure that our red blood cells don’t cause any problems within the
Osmosis is a natural occurrence constantly happening within the cells of all living things. For osmosis to occur, water molecules must move across a semipermeable membrane from an area of low concentration to an are of high concentration. In order to understand osmosis, people must understand the different types of concentrations that can be present within solution. One of them is an Isotonic solution where the concentration of dissolved particles is equal to that of a cell’s. Another is a hypertonic solution where there is a higher concentration of dissolved particles then inside the cell. And lastly there is a hypotonic solution where there are less dissolved particles than inside the cell. As dissolved particles move to a region of lower concentration, water moves the opposite direction as a result of there being less water in the highly concentrated region. In this experiment, gummy bears were placed in salt water, sugar water, and tap water to find the measure of osmosis between the solution and gummy bear.
Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.
What will happen to the egg when a student tests osmosis with 3 different soultions in a cup.The student is trying to see or find the effects of osmosis on an egg.Osmosis is the movement of water molecules through a selecfively permeable membrane. Diffuision is when something spreads more widely and equilibrium means when somethings is at rest due to equal opposite forces. The student wants to find out if the egg will shrink, fill up, or change in any way.
The concept of osmotic pressure must be understood when studying osmosis. The movement of water from a hypotonic solution through the membrane into a hypertonic solution can be prevented by applying force or pressure on the hypertonic side. The force that must be applied to prevent osmotic movement of water from hypotonic to hypertonic, measured in atmospheres, is referred to as osmotic pressure. Solutions with greater concentrations of OAS have greater osmotic pressures because greater force is required to prevent water movement into them. Distilled water has an osmotic pressure of zero.
A very carefully regulated process is solute concentration. If there is a sudden increase in water which enters the extracellular fluid, sodium ions will then contribute less to the extracellular solute concentration as the ratio between water and solute has now changed. Osmolality is the amount of solute in a kilogram, hence the osmolality in the extracellular space has also decreased.
Osmosis is the passive movement of water from an area of low solute concentration to an area of high solute concentration, normally across a membrane which prevents the movement of solvent. This is a process by which materials may move into, out of, or within cells. Osmosis doesn’t depend on energy provided by living organisms but is affected by the properties of the cell membrane. The rate of osmosis is dependent on such factors as temperature, pressure, molecular properties such as size and mass, and the concentration gradient. In osmosis, the relationship between a solute’s concentration outside of cell and inside of a cell is described in terms of the tonicity of the solution outside of the cell. A cell is in a hypotonic solution when the solute is more concentrated inside the cell and therefore water moves into the cell. In this solution the cell swells as water enters, this may continue until it ruptures or hemolyzes. In the reverse condition, the cell is in a hypertonic solution
Osmosis is a process in which molecules in a solvent pass across a semipermeable membrane into a more concentrated solution from a less concentrated one, attempting to make both sides isotonic or equal to each other. Isotonic can also be described as an equilibrium, where there is no net movement of the molecules. Osmosis is relevant in everyday life whether the general population is aware of it or not. It could be as simple as sitting in the pool too long and getting pruney fingers or as complex as a cholera infection in the intestinal cells that does not allow the intestinal cells
Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Which of the following is true of osmosis? You correctly answered: c. It is a type of diffusion. 2. Which of the following occurs when a hypertonic solution is added to cells? You correctly answered: d. The cells shrink. 3. The variable that affects osmotic pressure is You correctly answered: a. the concentration of nondiffusing solutes. 4. The net movement of water would be into the cell in a You correctly answered: b. hypotonic solution.
Diffusion and Osmosis Experiment with a Shell-Less Egg After Three days of Testing Methods with Water and Corn Syrup
Four eggs that were previously soaked in vinegar were placed into four beakers with different levels of a glucose solution. The four beakers were filled with distilled water, 0.5M glucose, 1.5M glucose and 2.0M glucose. After the eggs were placed in the solutions, they were left for 60 minutes but weighed every 15 minutes to record whether there was an increase or a decrease in mass. The various masses were recorded as well as the percent change in mass. These were then recorded as line graphs. From this it was able to be determined if the egg had been in a hypotonic, hypertonic or isotonic solution, thus being able to determine if osmosis had occurred.
In osmosis, the flow of the water from or to a cell depends on whether the cell is immersed in a solution that is isotonic, hypotonic, or hypertonic to the solution. If the cell is isotonic to a solution, this means that the solute concentration of a cell and its environment is the same and therefore there will be no movement of water. If the solute concentration is lower than that of the cell, then water will flow into the cell, causing it to expand. If the solute concentration is lower than that of the cell then water will flow out of the cell, causing it to shrink.
This experiment was used to examine the hypothesis that: Osmosis is dependent on the concentrations of the substances involved.