Animals Female C57Bl/6 mice (Charles River, Wilmington, MA), born and housed in the Research Support Facility at the University of Kansas Medical Center, were used for all experiments. Mice received water and food ad libitum and were housed on a 12-hour light cycle (600 to 1800 hours). All research was approved by the University of Kansas Medical Center Institutional Animal Care and Use Committee (protocol numbers 2013-2150 and 2016-2344) in compliance with the National Institute of Health Guide for the Care and Use of Laboratory Animals. Experimental groups and ages Three separate cohorts of naïve and NMS mice were used to avoid confounding effects of additional stressors. In the first group, mice underwent urinary bladder distension …show more content…
Estrous cycle stage was estimated by determining the ratios of nucleated epithelial cells, cornified epithelial cells, and leukocytes. Estrous cycle estimations were made following a single observation and verification of estrus was made following at least 4 consecutive days of observation. No correlations between the estimated stage of estrous cycle and the results from behavioral assays or histological and molecular analysis were observed (data not shown). Urinary bladder distension Urinary bladder distension (UBD) was performed as previously described8. Briefly, under inhaled isoflurane (4% induction, 2% maintenance), wire electrodes (0.003” diameter; Grass Technologies, West Warwick, RI) were implanted into the abdominal musculature and the bladder was catheterized intravesically via the urethra. Anesthesia was lowered until hind limb reflexes, but not escape behaviors, were present (~0.9% isoflurane). Body temperature was maintained at approximately 37C. Gas flow from a compressed nitrogen tank was manually adjusted and monitored using a dual-stage low delivery pressure regulator (Matheson-Linweld, Kansas City, MO) and delivered via a custom-made distension control device (The University of Iowa Medical Instruments, Iowa City, IA). Following verification of stable responses to 60mmHg, each pressure (15,
The novel Of Mice and Men takes place in a western ranch full of hard working, burly, and vicious men, which is of similarity to the rural farm setting of the poem “To A Mouse”. Similarity between the two works is also shown in the motif of the mouse in Of Mice and Men in which both the mouse and Lennie are being controlled by a greater force. More similarities between the two works include conflicts, characters, and theme.
The last half of the book continued to follow Cece working on making friends and dealing with how she feels about her hearing. She also moves and makes a new friend, Martha. She also makes friends with her neighborhood children. Martha is a grade below her and they get along great. Cece assumes that Martha doesn’t know that she is deaf, because it is summer and they aren’t in the same class together in school and hasn’t seen her wearing the Phonic Ear. However, she does know and simply doesn’t care. The neighborhood kids are also kind to her and there is a neighbor boy Mike Miller that she has a crush on. All is well until Martha hurts Cece’s eye and panics. Her eye heals but Martha is still too afraid to be around
What truly is the “American Dream”? Furthermore, during the Great Depression, even the concept of the “American Dream” was not readily available and was no more than a fallacy at the time. For the 2 protagonists in Of Mice And Men, their dream, like many others, was to “live off the fatta the land” and become independent. However, this was not such an easy task at the time, not just because of the rough economic times, but because people of that era still had World War 2 still very fresh in their minds, with the harbored hatred and untrust that came along with it. Nevertheless, for the millions who died in the course of the war, this “American Dream” was not only something worth living for it was something dying for too. However, in the novella Of Mice and Men by John Steinbeck, the author emphasizes that the dreams and hopes were delusions and has a grim outlook on living life in search of a dream.
“Listen to me, you crazy bastard,” “Don 't you even take a look at the bitch.In the book “Mice and Men” People in the story uses mean phases and words to use against people that is not the same as them or that does not look like them.In the quote it shines light on how people treat each other during the book and on how they act when they are near each other.In John Steinbeck 's Novel Of Mice and Men, the author illustrate that people discriminate against one another because they want to feel better of themselves and to gain self pride.
Discrimination is a problem that plagues those whose qualities are vulnerable. There are many examples of discrimination in the novel, Of Mice and Men. The characters face discrimination in many different ways including racial, age, gender, and disability. Crooks, the black stable buck, is the victim of racial discrimination. Candy, the old swamper, is a victim of the age discrimination. The victim of gender discrimination is Curley's wife because she is a woman. Life of the victims is hard because of the things they have to go through. Lastly, Lennie is mentally handicap so he discriminated against because of that.
“You have to fight to reach your dream. You have to sacrifice and work hard for it”You have to fight to reach your dream. You have to sacrifice .... The American dream a common theme that appears throughout John Steinbeck's book “Of Mice and Men” as well as the poem”The American Dream” by Amy H. from Wichita, Kansas. The book “Of Mice and Men”a novel that is about two friends in search for their dream, working their hardest in order to achieve their objective. “The American Dream” poem is a poem that illustrates a lifestyle that would be dreamt about. Often Things that might not be meant to have a similar theme do indeed have the same theme, in this case the American dream is a theme that both Of Mice and Men and The American Dream have in common.
Three. Everything in life comes in a group of three. Three musketeers. Three blind mice. Even the three wise men. My life is based on three things: playing, marching, and performing. All these tie together to make my life heartening. When I play my instrument in band, not even the band director, is helping me move my fingers to help me play a sweet melody. When I march, I feel alive. Marching develops the independent person inside of me because not everyone sticks around to help me look superior. As for performing, it’s my decision if I want to look mature and capable of doing my talent in front of a crowd. All these things help me build the person I am, independent.
Both Steinbeck and Kelly explore the idea that evil acts are sometimes driven by society and its treatment of us, but also that evil acts are not necessarily committed by evil people. Even though some characters in the play and the novella are complicit in and take part in terrible acts, we still feel some sympathy towards them and this due to the pressure forced upon them by society, the people they consider to be important and desire to conform. In this essay, I will be examining how the different contextual background of both Of Mice and Men and DNA affect the way both Curley’s Wife (OMAM) and Leah (DNA) characters are presented. I will also consider how the pressure of society compelled them into doing immoral things but we however sympathise with them.
Lennie and George, polar opposite bestfriends in terms of build, strength, and intelligence open the mesmerizing production of Of Mice and Men, directed by Mark Clements at the Milwaukee Rep. On the 18th of February, the cast and crew put on a successful show, stage adapted from the book Of Mice and Men by author and playwright John Steinbeck. The play, co-produced by the Milwaukee Repertory Theater and Arizona Theater Company, takes place in the era of the Great Depression, depicting the life of two unique, itinerant ranch workers, Lennie and George. Though dealing with a few heavy topics such as racism and sexism, the two and a half hour long show managed to sweep the audience in and keep them on their toes through their exceptional use of
As previously discussed, one of the major components that should guide the decision of bladder management devices is how it will affect the patient’s quality of life. A person’s perception of self, as well as their ability to bond with loved ones is a large factor in this. As mentioned previously Liu, Attar, Gall, Shah, and Craggs (2010) discuss that benefits of intermittent catheterization, when it is a realistic option, include an improved sense of independence. This higher level of functioning allows the patient to rely less on family or caregivers, while also giving the patient more of an ability to travel outside the home unassisted. Another benefit of the use of intermittent catheterization is that patients feel they are able to have closer interpersonal relationships than those with suprapubic or transurethral catheters in place (Sugimara, Arnold, English, and Moore, 2008). Since other bladder drainage methods require continuous drainage with an attached system, they may become cumbersome, and make it more difficult for patients to be physically closer to loved ones.
The play Of Mice and Men, written by John Steinbeck and directed by Mark Clements, produced by the Arizona Theatre Production Company and The Milwaukee Repertory and performed at The Milwaukee Repertory on February 18 was an overall success in terms of set and line delivery although a few forgotten lines and technical difficulties with the set took away some of the impact.
work and are applicable to humans. Other animals, such as rabbits, monkeys, and pigs, are also good animal models because of their similar physiological and anatomical features allow them to have a good construct validity and face validity (Crawley, 2012). Mice are similar to humans, small, and the least expensive to use for research. These three reasons are why mice are chosen as a model organism for Autism. Ultimately, the mice tend to score high and fit the validity, physiological, and anatomical features better for studying autism. Mice are becoming transgenic. The genetic material from a disease or phenomena can transfer into a mice’s DNA, and this is how mice are transgenic (Crawley, 2012). This property of mice allows there to be a
When you realized you had a major mouse infestation, you immediately laid out a whole slew of traps to get rid of them. And at first, it worked: you were catching mice almost daily. But now you go weeks without catching one. What happened?
Rodents can be found everywhere on earth. Among the several different types of rodents, the ones that mostly prefer to live near humans, being the most numerous, are the mice and rats. They live in colonies which comprise of complex hierarchies, wherein they form deep bonds, often putting their own lives at risk to save family and friends. Their best habitats are cavities, holes inside walls, in sewer pipes, in stores where foodstuffs are kept, in the fields and in garbage dumps. As a matter of fact, they love to live anywhere food is available and is convenient for them to put up a nest. In addition, they can easily adjust to any kind of climate and even wipe out original mammals occupying the same niche.
A review of the estrous cycle is necessary to understanding the protocols for estrus synchronization. The estrous cycle of the mare is the period from one ovulation to a subsequent ovulation. Horses are a seasonally polyestrous animal meaning that non-pregnant mares experience recurring estrous cycles. Further, the equine breeding season is regulated by photoperiod, light exposure. The mares’ estrous cycle is 21 to 22 days in length and involves hormones produced by the pineal gland, hypothalamus, pituitary gland, ovaries and endometrium.