. For parts (a) and (b) of this problem use the following standard reduction potentials, free energies, and nonequilibrium concentra- tions of reactants and products: АТР - 3.10 mм P; = 5.90 mM ADP = 220 µM glucose = 5.10 mM pyruvate = 62.0 uM NAD+ = 350 µM NADH = 15.0 μΜ CO, = 15.0 torr half reaction E (V) NAD+ + H+ + 2e→ NADH -0.315 2Pyruvate + 6H* + 4e → glucose -0.590 pyruvate + NADH + 2H+ AG" = -64.4 k)/mol → ethanol + NAD+ + CO, ATP + H,0→ ADP + P; + H* AG" - 30.5 kl/mol (a) Consider the last two steps in the alcoholic fermentation of glu- cose by brewer's yeast: pyruvate + NADH + 2H* » ethanol + NAD* + CO, Calculate the nonequilibrium concentration of ethanol in yeast cells, if AG = -38.3 kJ/mol for this reaction at pH=7.4 and 37 °C when the reactants and products are at the concentrations given above. (b) Consider the degradation of glucose to pyruvate by the glycolytic pathway: glucose + 2ADP + 2P; + 2NAD+ →2 pyruvate + 2ATP + 2H,0 + 2NADH + 2H* Calculate AG for this reaction at pH =7.4 and 37 °C.

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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. For parts (a) and (b) of this problem use the following standard
reduction potentials, free energies, and nonequilibrium concentra-
tions of reactants and products:
АТР - 3.10 mм
P; = 5.90 mM
ADP = 220 µM
glucose = 5.10 mM
pyruvate = 62.0 uM
NAD+ = 350 µM
NADH =
15.0 μΜ
CO, = 15.0 torr
half reaction
E (V)
NAD+ + H+ + 2e→ NADH
-0.315
2Pyruvate + 6H* + 4e →
glucose
-0.590
pyruvate + NADH + 2H+
AG" = -64.4 k)/mol
→ ethanol + NAD+ + CO,
ATP + H,0→ ADP + P; + H*
AG"
- 30.5 kl/mol
(a) Consider the last two steps in the alcoholic fermentation of glu-
cose by brewer's yeast:
pyruvate + NADH + 2H* » ethanol + NAD* + CO,
Calculate the nonequilibrium concentration of ethanol in yeast cells,
if AG = -38.3 kJ/mol for this reaction at pH=7.4 and 37 °C when
the reactants and products are at the concentrations given above.
(b) Consider the degradation of glucose to pyruvate by the glycolytic
pathway:
glucose + 2ADP + 2P; + 2NAD+ →2 pyruvate + 2ATP
+ 2H,0 + 2NADH + 2H*
Calculate AG for this reaction at pH =7.4 and 37 °C.
Transcribed Image Text:. For parts (a) and (b) of this problem use the following standard reduction potentials, free energies, and nonequilibrium concentra- tions of reactants and products: АТР - 3.10 mм P; = 5.90 mM ADP = 220 µM glucose = 5.10 mM pyruvate = 62.0 uM NAD+ = 350 µM NADH = 15.0 μΜ CO, = 15.0 torr half reaction E (V) NAD+ + H+ + 2e→ NADH -0.315 2Pyruvate + 6H* + 4e → glucose -0.590 pyruvate + NADH + 2H+ AG" = -64.4 k)/mol → ethanol + NAD+ + CO, ATP + H,0→ ADP + P; + H* AG" - 30.5 kl/mol (a) Consider the last two steps in the alcoholic fermentation of glu- cose by brewer's yeast: pyruvate + NADH + 2H* » ethanol + NAD* + CO, Calculate the nonequilibrium concentration of ethanol in yeast cells, if AG = -38.3 kJ/mol for this reaction at pH=7.4 and 37 °C when the reactants and products are at the concentrations given above. (b) Consider the degradation of glucose to pyruvate by the glycolytic pathway: glucose + 2ADP + 2P; + 2NAD+ →2 pyruvate + 2ATP + 2H,0 + 2NADH + 2H* Calculate AG for this reaction at pH =7.4 and 37 °C.
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